PUBCHEM_BIOASSAY: qHTS Assay for Identification of Novel General Anesthetics. In this assay, a GABAergic mimetic model system, apoferritin and a profluorescent 1-aminoanthracene ligand (1-AMA), was used to construct a competitive binding assay for identification of novel general anesthetics (Class of assay: confirmatory) [Related pubchem assays: 2385 (Probe Development Summary for Identification of Novel General Anesthetics), 2323 (Validation apoferritin assay run on SigmaAldrich LOPAC1280 collection)]
PubChem BioAssay. qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT. (Class of assay: confirmatory)
Binding affinity to human partial length TRIM24-phd-bromo (P790 to P977 residues) expressed in Escherichia coli BL21 at 100 uM after 1 hr by BROMOscan assay relative to control
Binding affinity to human partial length BRPF1 (E627 to G740 residues) expressed in Escherichia coli BL21 at 100 uM after 1 hr by BROMOscan assay relative to control
Inhibition of recombinant human N-terminal GST-tagged BRPF2 (556 to 688 residues) at 200 uM using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay
Inhibition of recombinant human N-terminal His-tagged BRPF3 (588 to 703 residues) at 200 uM using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay
Inhibition of recombinant human N-terminal GST-tagged ATAD2 (981 to 1146 residues) at 200 uM using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay
Inhibition of recombinant human N-terminal His-tagged BRD9 (135 to 248 residues) using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay
Inhibition of human GST-tagged BRD4 BD1 (44 to 170 residues) expressed in Escherichia coli BL21 using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay
Inhibition of recombinant human N-terminal GST-tagged CREBBP (1081 to 1197 residues) at 200 uM using biotinylated H4(1 to 21)K5/8/12/16Ac peptide as substrate after 30 mins by AlphaScreen assay