PubChem BioAssay. qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT. (Class of assay: confirmatory)
Activation of TLR4 in human PBMC cells assessed as AUC of IL8 release at 10 uM after 18 hrs by ELISA relative to N-cyclohexyl-2-(4-oxo-3-phenyl-4,5-dihydro-3H-pyrimido[5,4-b]indol-2-ylthio)acetamide
Activation of mouse TLR4 transfected in HEK293 cells after 18 hrs by SEAP assay relative to N-cyclohexyl-2-(4-oxo-3-phenyl-4,5-dihydro-3H-pyrimido[5,4-b]indol-2-ylthio)acetamide
Activation of human TLR4 transfected in HEK293 cells after 18 hrs by SEAP assay relative to N-cyclohexyl-2-(4-oxo-3-phenyl-4,5-dihydro-3H-pyrimido[5,4-b]indol-2-ylthio)acetamide
Activation of TLR4 in C57BL/6 mouse BMDC cells assessed as AUC of IP10 release at 5 uM after 18 hrs by ELISA relative to N-cyclohexyl-2-(4-oxo-3-phenyl-4,5-dihydro-3H-pyrimido[5,4-b]indol-2-ylthio)acetamide
Activation of TLR4 in C57BL/6 mouse BMDC cells assessed as AUC of IL6 release after 18 hrs by ELISA relative to N-cyclohexyl-2-(4-oxo-3-phenyl-4,5-dihydro-3H-pyrimido[5,4-b]indol-2-ylthio)acetamide