PubChem BioAssay. qHTS for Agonist of gsp, the Etiologic Mutation Responsible for Fibrous Dysplasia/McCune-Albright Syndrome: qHTS. (Class of assay: confirmatory)
Inhibition of Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells using DHO as substrate assessed as enzyme activity at 25 or 400 uM preincubated for 10 mins followed by substrate addition measured after 30 mins by DAMO-TSC reagent based colorimetric assay relative to control
Binding affinity to Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells assessed as change in melting temperature at 400 uM incubated for 10 mins by SYPRO orange dye based fluorescence thermal shift assay
Binding affinity to Bacillus anthracis Sterne His-tagged DHOase expressed in Escherichia coli BL21(DE3) Gold cells in presence of N-carbamyl-L-aspartate by surface plasmon resonance analysis