#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA656649	B	In vitro inhibitory activity against Catechol-O-methyltransferase from porcine liver; Uncompetitive mode of inhibition	Sus scrofa	4		assay format	Scientific Literature
2.	ALA3705870	B	Fluorometric Plate Assay: To determine the efficacy of various synthesized PAI-1 inhibitor compounds, a fluorometric plate assay was carried out to measure the half maximal inhibitory concentration (IC50) of these compounds on recombinant active human PAI-1 in vitro. An IC50 is a measure of the effectiveness of a compound in inhibiting biological or biochemical function. Stated another way, IC50 represents the concentration of a drug that is required for 50% inhibition in vitro. The IC50 of various compounds was measured using a fluorometric plate assay as set out below, and the results are shown in Table 3.Recombinant active human PAI-1 (Molecular Innovations) (final 1 nM) was incubated for 15 min at 23 C. with increasing concentrations of each compound in 100 mM NaCl, 40 mM HEPES, 0.005% Tween-20, 10% DMSO, pH 7.8. Alternately, the assay has been carried out using low concentrations of DMSO (about 0.1% DMSO or less) in the buffer. In various aspects, the invention does not require a solvent, like DMSO.	Homo sapiens	37		single protein format	BindingDB Database
3.	ALA5256982	T	Selectivity index, ratio of IC50 for cytotoxicity against HUVEC cells to IC50 for antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7		18		cell-based format	Scientific Literature
4.	ALA5256983	T	Cytotoxicity against HUVEC cells assessed as inhibition of cell growth incubated for 48 hrs by fluorescence based assay	Homo sapiens	17		cell-based format	Scientific Literature
5.	ALA5256984	T	Hemolytic activity in RBC (unknown origin) assessed as hemolysis at 100 ug/ml incubated for 1 hrs by absorbance based microplate reader analysis relative to control	Not specified	24		cell-based format	Scientific Literature
6.	ALA5256985	F	Selectivity index, ratio of IC50 for antiplasmodial activity against chloroquine-resistant Plasmodium falciparum FcB1 to IC50 for antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7		1		assay format	Scientific Literature
7.	ALA5256986	F	Selectivity index, ratio of IC50 for antiplasmodial activity against chloroquine-resistant Plasmodium falciparum FcB1 to IC50 for antiplasmodial activity against multidrug-resistant Plasmodium falciparum W2		1		assay format	Scientific Literature
8.	ALA5256987	F	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 assessed as intraerythrocytic growth inhibition	Plasmodium falciparum	24		organism-based format	Scientific Literature
9.	ALA5256988	F	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum FcB1 assessed as intraerythrocytic growth inhibition	Plasmodium falciparum	17		organism-based format	Scientific Literature
10.	ALA5256989	F	Antiplasmodial activity against multidrug-resistant Plasmodium falciparum W2 assessed as intraerythrocytic growth inhibition	Plasmodium falciparum	17		organism-based format	Scientific Literature
11.	ALA5256990	P	Aqueous solubility of compound assessed as Cmax incubated for 3 days by UV spectrophotometer based shake-flask method		16		small-molecule physicochemical format	Scientific Literature
12.	ALA5256992	T	Toxicity in zebrafish embryos assessed as survival at highest concentration measured for 3 days relative to control	Danio rerio	6		assay format	Scientific Literature
13.	ALA5256993	T	Toxicity in zebrafish embryos assessed as reduction in brain size at 10 to 100 ug/ml measured for 3 days	Danio rerio	6		tissue-based format	Scientific Literature
14.	ALA5256994	T	Toxicity in zebrafish embryos assessed as brain misshape at 10 to 100 ug/ml measured for 3 days	Danio rerio	6		tissue-based format	Scientific Literature
15.	ALA5256995	T	Toxicity in zebrafish embryos assessed as modified heart rate at 10 to 100 ug/ml measured for 3 days	Danio rerio	6		assay format	Scientific Literature
16.	ALA5256996	T	Toxicity in zebrafish embryos assessed as reduction in egg hatching measured for 72 hrs	Danio rerio	2		tissue-based format	Scientific Literature
17.	ALA5256998	T	Toxicity in zebrafish embryo assessed as egg hatching at 100 ug/ml measured for 72 hrs relative to control	Danio rerio	1		tissue-based format	Scientific Literature
18.	ALA5256999	T	Toxicity in zebrafish embryos assessed as reduction in egg hatching at 10 to 100 ug/ml measured 5 days post fertilization	Danio rerio	2		tissue-based format	Scientific Literature
19.	ALA5257000	T	Toxicity in zebrafish embryo assessed as developmental defects at 10 to 100 ug/ml measured 24 hrs post fertilization	Danio rerio	6		assay format	Scientific Literature
20.	ALA5257001	T	Toxicity in zebrafish embryo assessed as survival at 10 to 100 ug/ml measured 24 hrs post fertilization relative to control	Danio rerio	6		assay format	Scientific Literature