#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3425060	Binding	Displacement of [3H]nisoxetine from recombinant human norepinephrine transporter expressed in CHO cells at 1 uM after 120 mins by scintillation counting analysis relative to control	Homo sapiens	1	ALA3421625	cell-based format	Scientific Literature
2.	ALA3579131	Binding	Binding affinity to human NET at 1 uM	Homo sapiens	4	ALA3576859	single protein format	Scientific Literature
3.	ALA3582629	Binding	Displacement of [3H]nisoxetine from human recombinant NET expressed in HEK cell membranes by radioligand binding assay	Homo sapiens	1	ALA3580564	cell-based format	Scientific Literature
4.	ALA3587178	Binding	Inhibition of radioligand binding at human NET at 10 uM	Homo sapiens	1	ALA3585260	single protein format	Scientific Literature
5.	ALA3595873	Binding	Inhibition of human NET expressed in CHOK1 cells incubated for 45 mins by [3H]-norepinephrine uptake assay	Homo sapiens	17	ALA3593224	cell-based format	Scientific Literature
6.	ALA3602241	Binding	Inhibition of human NE transporter at 10 uM relative to control	Homo sapiens	1	ALA3600314	single protein format	Scientific Literature
7.	ALA3607556	Binding	Binding affinity to human NET expressed in HEK293 cells assessed as induction of [3H]MPP+ release by liquid scintillation counting method	Homo sapiens	1	ALA3603823	cell-based format	Scientific Literature
8.	ALA3610516	Binding	Binding affinity to NET (unknown origin) at 10 uM	Homo sapiens	1	ALA3608284	single protein format	Scientific Literature
9.	ALA3614495	Binding	Inhibition of human NET expressed in HEK293 cells assessed as reduction of serotonin reuptake using [3H]-NET at 1 uM after 15 mins by liquid scintillation counting relative to GBR-12909	Homo sapiens	10	ALA3611988	cell-based format	Scientific Literature
10.	ALA3614496	Binding	Inhibition of human NET expressed in HEK293 cells assessed as reduction of serotonin reuptake using [3H]-NET at 3 uM after 15 mins by liquid scintillation counting relative to venlafaxine HCl	Homo sapiens	14	ALA3611988	cell-based format	Scientific Literature
11.	ALA3614498	Binding	Inhibition of human NET expressed in HEK293 cells assessed as reduction of serotonin reuptake using [3H]-NET after 15 mins by liquid scintillation counting	Homo sapiens	11	ALA3611988	cell-based format	Scientific Literature
12.	ALA3614219	Binding	Binding affinity to human NE transporter assessed as inhibition of control specific binding at 10 uM	Homo sapiens	1	ALA3612012	single protein format	Scientific Literature
13.	ALA3616098	Binding	Displacement of [3H]-nisoxetine from norepinephrine transporter (unknown origin) at 0.1 mM relative to control	Homo sapiens	1	ALA3611968	single protein format	Scientific Literature
14.	ALA3616099	Binding	Displacement of [3H]-nisoxetine from norepinephrine transporter (unknown origin) at 1 mM relative to control	Homo sapiens	1	ALA3611968	single protein format	Scientific Literature
15.	ALA3616100	Binding	Displacement of [3H]-nisoxetine from norepinephrine transporter (unknown origin) at 10 mM relative to control	Homo sapiens	1	ALA3611968	single protein format	Scientific Literature
16.	ALA3616103	Binding	Displacement of [3H]-nisoxetine from norepinephrine transporter (unknown origin) at 1 to 10 mM	Homo sapiens	1	ALA3611968	single protein format	Scientific Literature
17.	ALA3624132	Binding	Inhibition of human NET at 10 uM relative to control	Homo sapiens	2	ALA3621160	single protein format	Scientific Literature
18.	ALA3637062	Binding	Binding affinity to norepinephrine transporter (unknown origin)	Homo sapiens	1	ALA3632475	single protein format	Scientific Literature
19.	ALA3636144	Binding	Displacement of [3H]-Nisoxetine from recombinant human NET at 10 uM after 1.5 hrs by Microbeta scintillation counting analysis relative to control	Homo sapiens	1	ALA3632459	single protein format	Scientific Literature
20.	ALA3705744	Binding	Radioligand Binding Assay: Compounds were dissolved in 100% DMSO at a concentration 100 times the desired highest assay concentration, serially diluted 1:3 in 100% DMSO, and 1.0 ul/well of each solution was dispensed to a Nunc polypropylene, round bottom, 384-well plate. 100% inhibition is defined with 1.0 ul/well of 10 mM desipramine dissolved in DMSO. 50 ul/well of a 2x membrane preparation (0.4 mg/ml in 50 mM Tris-Cl, pH 7.5, 120 mM NaCl, 5 mM KCl) and 50 ul/well of a 2x radioligand solution (4 nM [3H]nisoxetine in 50 mM Tris-Cl, pH 7.5, 120 mM NaCl, 5 mM KCl) were added to the well and the reaction incubated for 1 hour at room temperature. The contents of the assay plate were then transferred to a Millipore MultiscreenHTS GF/B filter plate which was pretreated with 0.5% PEI for at least one hour. The plate was vacuum filtered and washed with 7 washes of 100 ul/well 50 mM Tris-Cl, pH 7.5, 120 mM NaCl, 5 mM KCl chilled to 4 C.	Homo sapiens	55	ALA3639048	cell membrane format	BindingDB Database