# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA701630 | B | Selectivity towards Protein kinase, ILK1 | 1 | ALA1136209 | single protein format | Scientific Literature | ||
2. | ALA1244364 | B | Inhibition of ILK | 1 | ALA1240341 | single protein format | Scientific Literature | ||
3. | ALA1832059 | B | Inhibition of ILK in human PC3 cells assessed as decrease in GSK3-beta phosphorylation by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
4. | ALA1832060 | B | Inhibition of ILK in human MDA-MB-231 cells assessed as decrease in GSK3-beta phosphorylation by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
5. | ALA1832061 | B | Inhibition of ILK in human PC3 cells assessed as decrease in MLC phosphorylation by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
6. | ALA1832062 | B | Inhibition of ILK in human MDA-MB-231 cells assessed as decrease in MLC phosphorylation by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
7. | ALA1832067 | B | Inhibition of human ILK activity assessed myelin basic protein phosphorylation by radiometric kinase assay in the presence of [gamma 32P]ATP | Homo sapiens | 1 | ALA1828498 | single protein format | Scientific Literature | |
8. | ALA1832785 | B | Inhibition of ILK in human PC3 cells assessed as decrease in phosphorylated ERK1/2 concentration by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
9. | ALA1832786 | B | Inhibition of ILK in human PC3 cells assessed as decrease in phosphorylated p38 concentration by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
10. | ALA1832787 | B | Inhibition of ILK in human PC3 cells assessed as decrease in phosphorylated JNK concentration by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
11. | ALA1832788 | B | Inhibition of ILK in human PC3 cells expressing constitutively active ILK assessed as decrease in phosphorylated ERK1/2 concentration by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
12. | ALA1832789 | B | Inhibition of ILK in human PC3 cells expressing constitutively active ILK assessed as decrease in phosphorylated p38 concentration by Western blot analysis | Homo sapiens | 1 | ALA1828498 | cell-based format | Scientific Literature | |
13. | ALA1943604 | B | Inhibition of human ILK in HL-60 cells lysate assessed as reduction of labeling of acyl-phosphate ATP probe at 100 nM | Homo sapiens | 4 | ALA1938230 | cell-based format | Scientific Literature | |
14. | ALA2211509 | B | Inhibition of ILK | 1 | ALA2203257 | single protein format | Scientific Literature | ||
15. | ALA2422428 | B | Inhibition of ILK in human PC3 cell lysates at 10 uM using desthiobiotin-tagged ATP probe AX9989 followed by trypsinization by LC/MS analysis | Homo sapiens | 12 | ALA2417377 | single protein format | Scientific Literature | |
16. | ALA3419802 | B | Binding affinity to GST-tagged PH domain of ILK (unknown origin) by surface Plasmon resonance spectroscopy | Homo sapiens | 3 | ALA3414473 | single protein format | Scientific Literature | |
17. | ALA3705203 | B | In Vitro Ezyme Assay: 5 uL of each compound dilution were robotically pipetted to Costar serocluster plates maintaining the same plate layout. All assay mixtures consisted of the following volumes: 5 uL diluted compound, 10 uL target enzyme preparation, 1 uL substrate, 5 uL assay ATP. From each assay mixture, 10 uL of assay mixture was spotted onto Millipore Multiscreen-PH opaque plates and washed twice for 10 minutes in 1% phosphoric acid. The plates were dried at 40 C. for 30 minutes, then substrate-phosphate complexes were quantitated by scintillation counting. These Millipore plates are in a 96-well format with immobilized P81 phosphocellulose membranes in the wells. Both the phosphorylated and non-phosphorylated form of the substrate bind to the membrane while ATP (unincorporated phosphate) is removed by subsequent wash steps. | Homo sapiens | 151 | ALA3638706 | assay format | BindingDB Database | |
18. | ALA3734081 | B | Inhibition of full length recombinant GST-tagged ILK (unknown origin) expressed in baculovirus infected insect Sf9 cells using CKRRRLASLR as substrate incubated for 15 mins by scintillation counting analysis in presence of radiolabelled phosphate | Homo sapiens | 17 | ALA3727375 | cell-based format | Patent Bioactivity Data | |
19. | ALA3734121 | B | Inhibition of full-length recombinant human GST-tagged ILK1 expressed in baculovirus infected insect Hi5 cells using CKRRRLASLR-amide as substrate after 15 mins by scintillation counting analysis in presence of [gamma-32P]-ATP | Homo sapiens | 152 | ALA3733935 | cell-based format | Patent Bioactivity Data | |
20. | ALA3991737 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 217 | ALA3991601 | subcellular format | Kuster lab chemical proteomics drug profiling |