PUBCHEM_BIOASSAY: Mode of action assay-Automated electrophysiology assay of compounds that potentiate KCNQ2 potassium channel. (Class of assay: confirmatory) [Related pubchem assays: 2258 (Summary assay for the KCNQ2 potentiators.), 2558 (Automated patch clamp assay for KCNQ2 mutant W236L cell line with 936 test compounds, and 91 are found active.), 2239 (Primary HTS assay for KCNQ2 potentiators with 305606 compounds tested, and 1644 are found to be active.), 2287 (Confirmatory screen assay in duplicate for 1189 compounds identified through HTS, and 804 are found active.)]
PUBCHEM_BIOASSAY: Mode of action - deactivation constant concentration response for ztz240, a potentiator of KCNQ2 potassium channels. (Class of assay: confirmatory) [Related pubchem assays: 2258 (Summary assay for the KCNQ2 potentiators.), 2239 (Primary HTS assay for KCNQ2 potentiators with 305606 compounds tested, and 1644 are found to be active.)]
PUBCHEM_BIOASSAY: Mode of action assay - current amplitude concentration response for derivatives of ZTZ240, a potentiator of KCNQ2 potassium channels. (Class of assay: confirmatory) [Related pubchem assays: 2258 (Summary assay for the KCNQ2 potentiators.), 2239 (Primary HTS assay for KCNQ2 potentiators with 305606 compounds tested, and 1644 are found to be active.)]
PUBCHEM_BIOASSAY: Dose response assay for compounds that inhibit KCNQ2 potassium channels on automated electrophysiological assay II. (Class of assay: confirmatory)
PubChem BioAssay. SAR Analysis for the identification of inhibitors of the two-pore domain potassium channel TASK1 - Selectivity assay against KCNQ2: FluxOR Assay CRC. (Class of assay: confirmatory)
Activity at rat KCNQ2 expressed in CHOK1 cells assessed as ratio of amplitude of outward current in presence of test compound to amplitude of outward current in absence of test compound at 10 uM at -10 mV by whole cell patch clamp assay
Activation of rat homomeric KCNQ2 expressed in CHOK1 cells increase in current amplitude at 30 uM at -10 mV by whole cell patch clamp assay relative to control
Activity at rat KCNQ2 expressed in CHOK1 cells assessed as ratio of amplitude of outward current in presence of test compound to amplitude of outward current in absence of test compound at 10 uM at +60 mV by whole cell patch clamp assay