#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA831921	B	Inhibitory concentration against 7-methyl-GTP binding to eukaryotic translation initiation factor 4E		29		single protein format	Scientific Literature
2.	ALA2051182	B	Competitive inhibition of [3H]m7-GTP binding to human FLAG-His6 tagged eIF4E expressed in Escherichia coli by scintillation proximity assay	Homo sapiens	26		assay format	Scientific Literature
3.	ALA2051183	B	Ratio of m7-GMP IC50 to compound IC50 for human eIF4E	Homo sapiens	1		single protein format	Scientific Literature
4.	ALA925866	B	Binding affinity to eukaryotic translation initiation factor 4E		1		single protein format	Scientific Literature
5.	ALA1953936	B	Binding affinity to recombinant eIF4E by fluorescence quenching titration method		14		single protein format	Scientific Literature
6.	ALA1226425	B	Inhibition of eIF4E interaction with mRNA cap structure in presence of 0.6 mM m7 GDP at 50 uM by chemical cross-linking assay		1		single protein format	Scientific Literature
7.	ALA1226441	B	Inhibition of eIF4E RNA binding activity in presence of 0.6 mM m7GDP at 50 uM		1		single protein format	Scientific Literature
8.	ALA1249118	B	Inhibition of eIF-4E expressed in human FaDu cells assessed as inhibition of translational activation at 1 uM by luciferase reporter gene assay relative to control		18		cell-based format	Scientific Literature
9.	ALA1639015	B	Binding affinity to purified elF4E		2		single protein format	Scientific Literature
10.	ALA5253737	B	Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) at 100 uM by fluorescence polarization assay relative to control	Homo sapiens	22		single protein format	Scientific Literature
11.	ALA5253738	B	Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay	Homo sapiens	23		single protein format	Scientific Literature
12.	ALA5253739	B	Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation at 100 uM preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis relative to control	Homo sapiens	22		single protein format	Scientific Literature
13.	ALA5253740	B	Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis	Homo sapiens	23		single protein format	Scientific Literature
14.	ALA5253748	B	Inhibition of m7 GDP fluorescent probe binding to recombinant eIF4E (unknown origin) at 10 uM by fluorescence polarization assay relative to control	Homo sapiens	1		single protein format	Scientific Literature
15.	ALA5253749	B	Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation at 10 uM preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis relative to control	Homo sapiens	1		single protein format	Scientific Literature
16.	ALA5253753	B	Potency index, ratio of m7GMP Kd to test compound Kd for inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay	Homo sapiens	1		single protein format	Scientific Literature
17.	ALA3295044	B	Displacement of N-alpha-fluorescein tagged eIF4G-derived peptide from GST-DN26-eIF4E (unknown origin) by fluorescence polarization assay	Homo sapiens	27		single protein format	Scientific Literature
18.	ALA3295047	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as displacement of eIF4G by measuring enhanced 4E-BP1 binding to eIF4E at 30 uM after 3 hrs by m7GTP pull-down assay based western blot analysis	Homo sapiens	2		cell-based format	Scientific Literature
19.	ALA3378733	B	Competitive binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) assessed as inhibition of eIF4E/eIF4G interaction by fluorescence polarization assay relative to parent compound	Homo sapiens	54		assay format	Scientific Literature
20.	ALA3378734	B	Binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) by intrinsic tryptophan fluorescence quenching method	Homo sapiens	3		assay format	Scientific Literature