#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3423530	Binding	Inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate after 30 mins	Homo sapiens	5	ALA3421629	single protein format	Scientific Literature
2.	ALA3429273	Binding	Inhibition of monoglyceride lipase (unknown origin) at 100 uM incubated for 1 hr	Homo sapiens	1	ALA3425459	single protein format	Scientific Literature
3.	ALA3587056	Binding	Inhibition of human recombinant MAGL using 1,3-dihydroxypropan-1-yl 4-pyren-1-yl butanoate as substrate at 10 uM preincubated for 15 mins with substrate followed by protein addition measured after 45 mins by reverse phase HPLC with fluorescence detection method	Homo sapiens	5	ALA3585341	single protein format	Scientific Literature
4.	ALA3587057	Binding	Inhibition of human recombinant MAGL using 1,3-dihydroxypropan-1-yl 4-pyren-1-yl butanoate as substrate preincubated for 15 mins with substrate followed by protein addition measured after 45 mins by reverse phase HPLC with fluorescence detection method	Homo sapiens	3	ALA3585341	single protein format	Scientific Literature
5.	ALA3599583	Binding	Inhibition of MAGL (unknown origin) at 5 mM after 10 mins relative to control	Homo sapiens	5	ALA3596143	single protein format	Scientific Literature
6.	ALA3599584	Binding	Inhibition of MAGL (unknown origin) at 2 mM after 10 mins relative to control	Homo sapiens	1	ALA3596143	single protein format	Scientific Literature
7.	ALA3599585	Binding	Inhibition of MAGL (unknown origin) at 125 uM after 10 mins relative to control	Homo sapiens	2	ALA3596143	single protein format	Scientific Literature
8.	ALA3599586	Binding	Inhibition of MAGL (unknown origin) at 250 uM after 10 mins relative to control	Homo sapiens	2	ALA3596143	single protein format	Scientific Literature
9.	ALA3599587	Binding	Inhibition of MAGL (unknown origin) at 500 uM after 10 mins relative to control	Homo sapiens	2	ALA3596143	single protein format	Scientific Literature
10.	ALA3599588	Binding	Inhibition of MAGL (unknown origin) after 10 mins	Homo sapiens	1	ALA3596143	single protein format	Scientific Literature
11.	ALA3614384	Binding	Inhibition of human MAGL expressed in HEK293 cells at 10 uM pre-incubated for 10 mins before 2-AG substrate addition by HPLC method	Homo sapiens	29	ALA3612019	cell-based format	Scientific Literature
12.	ALA3614385	Binding	Inhibition of human MAGL expressed in HEK293 cells pre-incubated for 10 mins before 2-AG substrate addition by HPLC method	Homo sapiens	9	ALA3612019	cell-based format	Scientific Literature
13.	ALA3614386	Binding	Inhibition of human MAGL expressed in HEK293 cells at 20 uM pre-incubated for 10 mins before 2-AG substrate addition by HPLC method	Homo sapiens	1	ALA3612019	cell-based format	Scientific Literature
14.	ALA3705751	Binding	Enzyme Assay: All rate-based assays were performed in black 384-well polypropylene polymerase chain reaction (PCR) microplates (Abgene) in a total volume of 30 μL. Substrate 4-methylumbelliferyl butyrate (4MU-B; Sigma) and either purified mutant MGL (mut-MGLL 11-313 L179S L186S) or purified wild type MGL (wt-MGLL 6H-11-313) were diluted separately into 20 mM PIPES buffer (pH=7.0), containing 150 mM NaCl and 0.001% Tween 20. Compounds of formula (I) were pre-dispensed (50 nL) into the microplate using a liquid handling dispenser prior to adding 4MU-B (25 μL of 1.2× solution to a final concentration of 10 μM) followed by enzyme (5 μL of a 6× solution to a final concentration of 5 nM) to initiate the reaction. Final compound concentrations ranged from 17 to 0.0003 μM. The fluorescence change due to 4MU-B cleavage was monitored with excitation and emission wavelengths of 335 and 440 nm, respectively, and a bandwidth of 10 nm (Safire2, Tecan) at 37° C. for 5 min.	Homo sapiens	1	ALA3639198	single protein format	BindingDB Database
15.	ALA3705752	Binding	ThermoFluor Assay: The ThermoFluor (TF) assay is a 384-well microplate-based binding assay that measures thermal stability of proteins1,2. The experiments were carried out using ThermoFluor instruments available from Johnson & Johnson Pharmaceutical Research & Development, LLC. TF dye used in all experiments was 1,8-ANS (Invitrogen: A-47). Final TF assay conditions used for MGL studies were 0.07 mg/ml of mutant MGL, 100 uM ANS, 200 mM NaCl, 0.001% Tween-20 in 50 mM PIPES (pH=7.0).Screening compound plates contained 100% DMSO compound solutions at a single concentration. For follow-up concentration-response studies, compounds were arranged in a pre-dispensed plate (Greiner Bio-one: 781280), wherein compounds were serially diluted in 100% DMSO across 11 columns within a series. Columns 12 and 24 were used as DMSO reference and contained no compound. For both single and multiple compound concentration-repsonse experiments, the compound aliquots (46 mL) were robotically predispensed directly into 384-well.	Homo sapiens	1	ALA3639198	single protein format	BindingDB Database
16.	ALA3749323	Binding	Inhibition of human MGL expressed in HEK239 cells using 2AG as substrate assessed as residual activity at 10 uM preincubated for 10 mins followed by substrate addition measured after 10 mins by HPLC analysis	Homo sapiens	27	ALA3745614	cell-based format	Scientific Literature
17.	ALA3768672	Binding	Inhibition of MAGL (unknown origin) at 30 uM	Homo sapiens	5	ALA3763072	single protein format	Scientific Literature
18.	ALA3791725	Binding	Inhibition of recombinant C-terminal His6-tagged human MAGL expressed in Escherichia coli using 2-arachidonoyl-[3H]-glycerol as substrate incubated for 20 mins by scintillation counting method	Homo sapiens	14	ALA3785023	assay format	Scientific Literature
19.	ALA3791726	Binding	Inhibition of human MAGL expressed in African green monkey COS cells using 2-arachidonoyl-[3H]-glycerol as substrate incubated for 20 mins by scintillation counting method	Homo sapiens	1	ALA3785023	cell-based format	Scientific Literature
20.	ALA3791993	Binding	Inhibition of recombinant C-terminal His6-tagged human MAGL expressed in Escherichia coli using 2-arachidonoyl-[3H]-glycerol as substrate preincubated for 10 mins followed by substrate addition measured after 20 mins by scintillation counting method	Homo sapiens	3	ALA3785023	assay format	Scientific Literature