#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA620735	B	Binding affinity towards mouse 5-hydroxytryptamine 7 receptor was evaluated using [3H]5-HT as radioligand	Mus musculus	1	ALA1145819	single protein format	Scientific Literature
2.	ALA3857982	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 100 nM after 30 mins by DAPI staining based microscopy	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
3.	ALA3857985	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 100 nM after 30 mins by DAPI staining based microscopy	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
4.	ALA3857986	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 100 nM after 2 hrs by DAPI staining based microscopy relative to control	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
5.	ALA3857987	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 100 nM after 4 hrs by DAPI staining based microscopy	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
6.	ALA3857988	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 20 nM after 30 mins by DAPI staining based microscopy relative to control	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
7.	ALA3857989	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 20 nM up to 4 hrs by DAPI staining based microscopy relative to control	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
8.	ALA3857990	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 100 nM after 2 hrs in presence of 5-HT7 receptor antagonist SB-269970 by DAPI staining based microscopic analysis	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
9.	ALA3857991	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 100 nM after 2 hrs by DAPI staining based microscopy relative to control	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
10.	ALA3857992	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 100 nM up to 24 hrs by DAPI staining based microscopy	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
11.	ALA3857993	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 20 nM after 30 mins by DAPI staining based microscopy relative to control	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
12.	ALA3857994	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 20 nM up to 24 hrs by DAPI staining based microscopy	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
13.	ALA3857995	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse striatum assessed as increase in neurite length at 20 nM after 2 hrs in presence of 5-HT7 receptor antagonist SB-269970 by DAPI staining based microscopic analysis	Mus musculus	1	ALA3856221	single protein format	Scientific Literature
14.	ALA3857996	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 100 nM after 2 hrs in presence of 5-HT7 receptor antagonist SB-269970 by DAPI staining based microscopic analysis	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
15.	ALA3857997	B	Agonist activity at 5-HT7 receptor in embryonic day 15 C57BL/6 mouse cortex assessed as increase in neurite length at 20 nM after 2 hrs in presence of 5-HT7 receptor antagonist SB-269970 by DAPI staining based microscopic analysis	Mus musculus	1	ALA3856221	tissue-based format	Scientific Literature
16.	ALA3887410	B	Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.		2	ALA3886311	assay format	BindingDB Database
17.	ALA4770550	B	Agonist activity at 5HT7 receptor in C57BL/6 mouse hippocampal neurons assessed as reversal of DHPG induced mGluR-dependent long-term depression by measuring excitatory postsynaptic current at -70 mV holding potential at 100 nM pretreated with DHPG for 5 mins after DHPG washout compound addition measured after 40 mins by whole cell patch clamp assay (Rvb = 67.7 +/- 8 %)	Mus musculus	2	ALA4765335	tissue-based format	Scientific Literature