# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA685164 | B | In vitro inhibition of porcine Glycolate oxidase | Sus scrofa | 15 | single protein format | Scientific Literature | ||
2. | ALA4234427 | B | Inhibition of human N-terminal His-tagged glycolate oxidase expressed in C41(D43) Escherichia coli using glycolate as substrate preincubated for 30 mins followed by substrate addition by DCIP dye-based assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
3. | ALA4418446 | B | Inhibition of recombinant human liver HAOX1 using glycolate as substrate preincubated for 5 mins followed by substrate addition in presence of 2,6-dichlorophenol | Homo sapiens | 1 | assay format | Patent Bioactivity Data | ||
4. | ALA4827633 | B | Inhibition of recombinant human GOX expressed in Escherichia coli C41 (DE3) using glycolate as substrate preincubated for 10 mins followed by substrate addition measured by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 7 | assay format | Scientific Literature | ||
5. | ALA4827645 | B | Inhibition of human glycolate oxidase expressed in Escherichia coli using glycolate as substrate by DCIP dye based spectrophotometry analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
6. | ALA5036693 | B | Inhibition of human recombinant HAO1 assessed as detecting hydrogen peroxide using glycolic acid as substrate preincubated for 30 min followed by substrate addition measured after 15 mins by microplate reader | Homo sapiens | 33 | single protein format | Scientific Literature | ||
7. | ALA5036694 | B | Inhibition of human recombinant HAO1 assessed as fluorescent intensity using hydrogen peroxide as substrate by HRP interference detection assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
8. | ALA5036702 | B | Inhibition of human recombinant HAO1 expressed in CHO cells co-transformed with a vector expressing glycolate oxidase assessed as cell rescue from CHO-Go induced oxalate mediated cell death at 30 uM incubated for 1 hr followed by addition of glycolic acid and measured upto 96 hrs | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
9. | ALA5036708 | B | Inhibition of human recombinant HAO1 expressed in CHO cells co-transformed with a vector expressing glycolate oxidase assessed as cell rescue from CHO-Go induced oxalate mediated cell death incubated for 1 hr followed by addition of glycolic acid and measured upto 96 hrs | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
10. | ALA5123584 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 5 mins followed by substrate addition and measured after 1 min in the presence of DCHBS by horseradish peroxidase coupled colorimetric method relative to control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
11. | ALA5123586 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
12. | ALA5123589 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
13. | ALA5123594 | B | Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
14. | ALA5123596 | B | Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 10 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
15. | ALA5123597 | B | Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 100 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
16. | ALA5123604 | B | Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in Vmax with increase in apparent Km using glycolate as substrate upto 60 microM | Homo sapiens | 4 | cell-based format | Scientific Literature |