# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1050561 | Binding | Inhibition of human PGES2 | Homo sapiens | 3 | ALA1156058 | single protein format | Scientific Literature | |
2. | ALA958746 | Binding | Inhibition of mPGES2 | 2 | ALA1157389 | single protein format | Scientific Literature | ||
3. | ALA876474 | Binding | In vitro inhibition against recombinant human mPGES-2 | Homo sapiens | 2 | ALA1140249 | single protein format | Scientific Literature | |
4. | ALA829449 | Binding | Inhibitory concentration against human prostaglandin E2 synthase (mPGES-1) | Homo sapiens | 32 | ALA1140249 | single protein format | Scientific Literature | |
5. | ALA945933 | Binding | Inhibition of human mPGES2 | Homo sapiens | 2 | ALA1142217 | single protein format | Scientific Literature | |
6. | ALA1695096 | Binding | Inhibition of Prostaglandin E2 synthase-1 in IL1-beta stimulated microsomal fraction of human A549 cell assessed as PGE2 level by RP-HPLC | Homo sapiens | 15 | ALA1687920 | microsome format | Scientific Literature | |
7. | ALA1695097 | Binding | Inhibition of Prostaglandin E2 synthase-1 in IL1-beta stimulated microsomal fraction of human A549 cell assessed as residual activity at 30 uM | Homo sapiens | 15 | ALA1687920 | microsome format | Scientific Literature | |
8. | ALA3858510 | Binding | Inhibition of human mPGES-2 | Homo sapiens | 3 | ALA3856235 | single protein format | Scientific Literature | |
9. | ALA3389828 | Binding | Inhibition of recombinant mPGES-2 (unknown origin) at 10 uM | Homo sapiens | 1 | ALA3351804 | single protein format | Scientific Literature | |
10. | ALA3873620 | Binding | Inhibition of human recombinant mPGES-2 expressed in CHO cells assessed as reduction in conversion of PGH2 to PGE2 at 10 uM incubated for 10 mins followed by substrate addition measured after 1 min | Homo sapiens | 1 | ALA3872236 | cell-based format | Scientific Literature | |
11. | ALA3887295 | Binding | Biochemical Assay: A solution of test compound was added to a diluted microsome preparation containing human mPGES-1 and pre-incubated for 15 minutes in potassium phosphate buffer pH 6.8 with cofactor glutathione (GSH). Corresponding solutions without test compound were used as positive controls, and corresponding solutions without test compound and without microsomes were used as negative controls. The enzymatic reaction was then started by addition of the substrate PGH2 in an organic solution (dry acetonitrile).The typical reaction conditions of the enzymatic reaction were thus: Test compound: ranging from 60 uM to 0.002 uM, or zero in positive and negative controls; potassium phosphate buffer pH 6.8: 50 mM; GSH: 2.5 mM; mPGES-1-containing microsomes: 2 ug/mL (sample and positive controls) or 0 ug/mL (negative control); PGH2: 10.8 uM; Acetonitrile: 7.7% (v/v); DMSO: 0.6% (v/v). The reaction was stopped after one minute by adding an acidic solution (pH 1.9) of ferric chloride. | 239 | ALA3886264 | assay format | BindingDB Database | ||
12. | ALA4181305 | Binding | Inhibition of mPGES2 in human THP1 cells assessed as reduction in A23187-stimulated PGE2 production at 10 uM relative to control | Homo sapiens | 2 | ALA4177660 | cell-based format | Scientific Literature | |
13. | ALA4221876 | Binding | Inhibition of mPGES2 in A23187-stimulated human THP1 cells assessed as decrease in PGE2 production at 10 uM using arachidonic acid as substrate relative to control | Homo sapiens | 6 | ALA4219124 | assay format | Scientific Literature | |
14. | ALA4341393 | Binding | Binding affinity to PTGES2 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis | Homo sapiens | 2 | ALA4340528 | subcellular format | Scientific Literature | |
15. | ALA4341409 | Binding | Binding affinity to PTGES2 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis relative to untreated control | Homo sapiens | 2 | ALA4340528 | subcellular format | Scientific Literature | |
16. | ALA4402250 | Binding | Inhibition of mPGES (unknown origin) up to 30000 nM using PGH2 as substrate measured after 90 to 120 secs by RapidFire high-throughput mass spectrometry assay | Homo sapiens | 1 | ALA4400569 | single protein format | Scientific Literature |