# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3429884 | Binding | Inhibition of human Axl | Homo sapiens | 1 | ALA3425429 | single protein format | Scientific Literature | |
2. | ALA3428301 | Binding | Inhibition of human AXL using EAIYAAPFAKKK peptide substrate at 1 uM by 33P Hotspot assay | Homo sapiens | 1 | ALA3425491 | single protein format | Scientific Literature | |
3. | ALA3583345 | Binding | Inhibition of Axl (unknown origin) using polu (Glu,Tyr)4:1 substrate after 30 mins incubation by multi-well spectrophotometry | Homo sapiens | 1 | ALA3580555 | single protein format | Scientific Literature | |
4. | ALA3595506 | Binding | Inhibition of human AXL at 0.1 uM relative to control | Homo sapiens | 1 | ALA3593229 | single protein format | Scientific Literature | |
5. | ALA3625826 | Binding | Inhibition of human recombinant Axl | Homo sapiens | 1 | ALA3621115 | single protein format | Scientific Literature | |
6. | ALA3630634 | Binding | Inhibition of AXL (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control | Homo sapiens | 1 | ALA3627674 | single protein format | Scientific Literature | |
7. | ALA3632163 | Binding | Inhibition of AXL (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay | Homo sapiens | 3 | ALA3627608 | single protein format | Scientific Literature | |
8. | ALA3705826 | Binding | Binding Assay: The principle behind this assay is based upon the binding and displacement of an Alexa Fluor 647-labeled tracer to the kinase of interest. Binding of the tracer to the kinase is detected using an EU-labeled anti-tag antibody. Simultaneous binding of both the tracer and antibody to the kinase gives rise to a FRET-signal. Binding of an inhibitor to the kinase competes for binding with the tracer, resulting in a loss of FRET-signal. At first a compound according to formula (I) was diluted in 20 mM Hepes pH 8.0, 1 mM DTT, 10 mM MgCl2 and 0.01% Brij35. Next Axl kinase (5 nM final concentration; Proqinase), kinase tracer (15 nM final concentration; Invitrogen) and LanthaScreen Eu-Anti-GST antibody (2 nM final concentration; Invitrogen) was mixed with suitable compound dilutions and incubated for 1 hour. FRET-signal was quantified employing an EnVision Multilabelreader 2104 (Perkin Elmer). | 16 | ALA3639121 | single protein format | BindingDB Database | ||
9. | ALA3751443 | Binding | Inhibition of AXL (unknown origin) at 10 uM | Homo sapiens | 9 | ALA3745672 | single protein format | Scientific Literature | |
10. | ALA3750468 | Binding | Inhibition of Axl (unknown origin) at 1 uM | Homo sapiens | 1 | ALA3745631 | single protein format | Scientific Literature | |
11. | ALA3750783 | Binding | Inhibition of AXL (unknown origin) at 1 uM | Homo sapiens | 1 | ALA3745672 | single protein format | Scientific Literature | |
12. | ALA3748628 | Binding | Inhibition of human AXL using [EAIYAAPFAKKK] as substrate | Homo sapiens | 1 | ALA3745707 | single protein format | Scientific Literature | |
13. | ALA3762052 | Binding | Inhibition of recombinant human Axl using poly (Glu,Tyr)4:1 as substrate after 60 mins by ELISA | Homo sapiens | 2 | ALA3758137 | single protein format | Scientific Literature | |
14. | ALA3762153 | Binding | Inhibition of AXL (unknown origin) at 100 nM | Homo sapiens | 1 | ALA3758169 | single protein format | Scientific Literature | |
15. | ALA3766485 | Binding | Inhibition of human AXL using poly(Glu, Tyr) as substrate | Homo sapiens | 1 | ALA3763130 | single protein format | Scientific Literature | |
16. | ALA3766489 | Binding | Inhibition of AXL phosphorylation in human MDA-MB-231 cells incubated for 1 to 3 hrs | Homo sapiens | 1 | ALA3763130 | cell-based format | Scientific Literature | |
17. | ALA3767452 | Binding | Inhibition of Axl (unknown origin) | Homo sapiens | 1 | ALA3763130 | single protein format | Scientific Literature | |
18. | ALA3773983 | Binding | Inhibition of human recombinant AXL assessed as residual activity at 10 uM after 1 hr by qPCR analysis relative to control | Homo sapiens | 1 | ALA3769377 | single protein format | Scientific Literature | |
19. | ALA3776886 | Binding | Inhibition of N-terminal His6-tagged recombinant human AXL expressed in baculovirus infected insect Sf9 cells assessed as remaining activity at 1 uM relative to control | Homo sapiens | 3 | ALA3774357 | cell-based format | Scientific Literature | |
20. | ALA3782149 | Binding | Inhibition of partial length human AXL expressed in bacterial system at 1 uM | Homo sapiens | 1 | ALA3779767 | assay format | Scientific Literature |