#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3791886	Binding	Binding affinity to recombinant human TLR4 at 6.25 to 100 uM by surface plasmon resonance analysis	Homo sapiens	1	ALA3784925	single protein format	Scientific Literature
2.	ALA3820858	Binding	Agonist activity at human TLR4 expressed in HEK cells assessed as increase in IL-8 production at 25 to 100 nM incubated for 24 hrs by ELISA	Homo sapiens	4	ALA3817818	cell-based format	Scientific Literature
3.	ALA3825658	Binding	Activation of TLR4 receptor in human monocytes assessed as inhibition of LPS-stimulated TNF-alpha production preincubated for 30 mins prior to LPS stimulation at 1 uM measured after 6 hrs by immunoassay	Homo sapiens	1	ALA3822396	cell-based format	Scientific Literature
4.	ALA854334	Functional	Antagonistic potency at human TLR4 expressed in HEK293 cells	Homo sapiens	4	ALA1141958	cell-based format	Scientific Literature
5.	ALA867199	Functional	Inhibition of induction of NF-kappaB by LPS in HEK293 cells co-transfected with TLR4, CD14 and MD2	Homo sapiens	12	ALA1139003	cell-based format	Scientific Literature
6.	ALA1226065	Binding	Inhibition of TLR4 binding to MD4		2	ALA1221358	single protein format	Scientific Literature
7.	ALA1827811	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of IL-8 release preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
8.	ALA1827812	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of TNFalpha release preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
9.	ALA1827813	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of IL-6 release preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
10.	ALA1827814	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of TNFalpha release at 250 uM preincubated for 5 to 10 mins	Homo sapiens	1	ALA1821673	single protein format	Scientific Literature
11.	ALA1827815	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of IL-8 release at 500 uM preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
12.	ALA1827816	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of TNFalpha release at 500 uM preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
13.	ALA1827817	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of IL-6 release at 500 uM preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
14.	ALA1827818	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as inhibition of IL-6 release at 250 uM preincubated for 5 to 10 mins	Homo sapiens	2	ALA1821673	single protein format	Scientific Literature
15.	ALA1827819	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as reduction in plasma IL-8 level at 250 uM preincubated for 5 to 10 mins	Homo sapiens	1	ALA1821673	single protein format	Scientific Literature
16.	ALA1827820	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as reduction in plasma TNFalpha level at 250 uM preincubated for 5 to 10 mins	Homo sapiens	1	ALA1821673	single protein format	Scientific Literature
17.	ALA1827821	Binding	Inhibition of LPS-induced TLR4 activation in human whole blood assessed as reduction in plasma IL-6 level at 250 uM preincubated for 5 to 10 mins	Homo sapiens	1	ALA1821673	single protein format	Scientific Literature
18.	ALA4356426	Binding	Inhibition of human TLR4 expressed in HEK293 cells assessed as reduction in LPS-induced NFkappaB production at 1.5 to 5 uM incubated for 16 hrs by quanti-blue SEAP assay	Homo sapiens	2	ALA4354819	cell-based format	Scientific Literature
19.	ALA4359300	Binding	Inhibition of human TLR4 expressed in HEK-Blue cells assessed as inhibition of LPS-induced NF-kappaB activation at 20 uM measured after 24 hrs by quanti-blue based SEAP reporter gene assay	Homo sapiens	1	ALA4354873	cell-based format	Scientific Literature
20.	ALA1913436	Functional	Partial agonist activity at TLR4 transfected in Sf9 insect cells assessed as inhibition of LPS-mediated sTLR4/MD2 homodimerization after 1 hr by native PAGE		1	ALA1909619	cell-based format	Scientific Literature