# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA936097 | B | Activity of human recombinant nm23H2 | Homo sapiens | 4 | ALA1145492 | single protein format | Scientific Literature | |
2. | ALA935323 | B | Activity of human recombinant nm23H2 assessed as conversion of 1 umol of ADP to ATP per min | Homo sapiens | 4 | ALA1145492 | single protein format | Scientific Literature | |
3. | ALA935324 | B | Activity of human recombinant nm23H2 assessed as phosphorylation efficiency relative to 4-ethynyl D4TDP | Homo sapiens | 3 | ALA1145492 | single protein format | Scientific Literature | |
4. | ALA3991804 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 17 | ALA3991601 | subcellular format | Kuster lab chemical proteomics drug profiling | |
5. | ALA3999071 | B | Binding affinity to NT647-labeled His-tagged NM23-H2 (unknown origin) expressed in Escherichia coli BL21(DE3) up to 500 uM after 30 mins by microscale thermophoresis method | Homo sapiens | 6 | ALA3997802 | assay format | Scientific Literature | |
6. | ALA3999072 | B | Binding affinity to NT647-labeled His-tagged NM23-H2 (unknown origin) expressed in Escherichia coli BL21(DE3) after 30 mins by microscale thermophoresis method | Homo sapiens | 2 | ALA3997802 | assay format | Scientific Literature | |
7. | ALA4018533 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 F60A mutant (unknown origin) up to 50 uM by SPR analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
8. | ALA4018534 | B | Binding affinity to NM23-H2 in human SiHa cells assessed as decrease in NM23-H2 to c-myc G-quadruplex DNA interaction after 9 hrs by Chromatin immunoprecipitation assay | Homo sapiens | 1 | ALA4017489 | cell-based format | Scientific Literature | |
9. | ALA4018536 | B | Binding affinity to NM23-H2 F60A mutant (unknown origin) up to 50 uM by SPR analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
10. | ALA4018538 | B | Binding affinity to NM23-H2 (unknown origin) by SPR analysis | Homo sapiens | 31 | ALA4017489 | single protein format | Scientific Literature | |
11. | ALA4018539 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 (unknown origin) after 30 mins by MST analysis | Homo sapiens | 47 | ALA4017489 | single protein format | Scientific Literature | |
12. | ALA4018540 | B | Binding affinity to NM23-H2 D54A mutant (unknown origin) by SPR analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
13. | ALA4018541 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 D54A mutant (unknown origin) after 30 mins by MST analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
14. | ALA4018542 | B | Binding affinity to NM23-H2 G113F mutant (unknown origin) by SPR analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
15. | ALA4018543 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 G113F mutant (unknown origin) after 30 mins by MST analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
16. | ALA4018544 | B | Binding affinity to NM23-H2 H118F mutant (unknown origin) by SPR analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
17. | ALA4018545 | B | Binding affinity to N-terminal NT-647-NHS labeled NM23-H2 H118F mutant (unknown origin) after 30 mins by MST analysis | Homo sapiens | 1 | ALA4017489 | assay format | Scientific Literature | |
18. | ALA4018551 | B | Binding affinity to NM23-H2 (unknown origin) assessed as ratio of Kd for protein interaction with biotin-labeled Pu22 c-myc G-quadruplex DNA in presence and absence of compound after 1 hr by ELISA | Homo sapiens | 17 | ALA4017489 | single protein format | Scientific Literature | |
19. | ALA4018552 | B | Binding affinity to NM23-H2 (unknown origin) assessed as reduction in protein interaction with Pu22 c-myc G-quadruplex DNA after 1 hr by EMSA | Homo sapiens | 1 | ALA4017489 | single protein format | Scientific Literature | |
20. | ALA4018555 | B | Binding affinity to NM23-H2 in human SiHa cells assessed as disruption of protein interaction with c-myc G-quadruplex DNA by measuring decrease in c-MYC mRNA level after 9 hrs by RT-PCR analysis | Homo sapiens | 1 | ALA4017489 | cell-based format | Scientific Literature |