#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706216	Binding	Radioligand Binding Assay: HEK293 cells stably expressing rat CB2 receptors were grown until a confluent monolayer was formed. Briefly, the cells were harvested and homogenized in TE buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA) using a polytron for 2x 10 second bursts in the presence of protease inhibitors, followed by centrifugation at 45,000x g for 20 minutes. The final membrane pellet was re-homogenized in storage buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA and 10% sucrose) and frozen at -78 C. until used. Saturation binding reactions were initiated by the addition of membrane preparation (protein concentration of 20 ug/well for rat CB2) into wells of a deep well plate containing [3H]CP 55,940 (120 Ci/mmol, a nonselective CB agonist commercially available from Tocris) in assay buffer (50 mM Tris, 2.5 mM EDTA, 5 mM MgCl2, and 0.5 mg/mL fatty acid free BSA, pH 7.4). After 45 min incubation at 30u C., binding reaction was terminated by the addition of 300 ul/well of cold assay buffer.		64	ALA3639359	cell-based format	BindingDB Database
2.	ALA3707666	Binding	Radioligand Binding Assays: The CB1 and CB2 radioligand binding assays described herein are utilized to ascertain the selectivity of compounds of the present application for binding to CB2 relative to CB1 receptors.HEK293 cells stably expressing human CB2 receptors were grown until a confluent monolayer was formed. Briefly, the cells were harvested and homogenized in TE buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA) using a polytron for 210 second bursts in the presence of protease inhibitors, followed by centrifugation at 45,000xg for 20 minutes. The final membrane pellet was re-homogenized in storage buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA and 10% sucrose) and frozen at -78 C. until used. Saturation binding reactions were initiated by the addition of membrane preparation (protein concentration of 5 ug/well for human CB2) into wells of a deep well plate containing [3H]CP 55,940 (120 Ci/mmol, a nonselective CB agonist commercially available from Tocris) in assay buffer.		38	ALA3638829	cell-based format	BindingDB Database
3.	ALA3707501	Binding	Radioligand Binding Assay: HEK293 cells stably expressing human CB2 receptors were grown until a confluent monolayer was formed. Briefly, the cells were harvested and homogenized in TE buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA) using a polytron for 2X10 second bursts in the presence of protease inhibitors, followed by centrifugation at 45,000 ug for 20 minutes. The final membrane pellet was re-homogenized in storage buffer (50 mM Tris-HCl, 1 mM MgCl2, and 1 mM EDTA and 10% sucrose) and frozen at -78 C. until used. Saturation binding reactions were initiated by the addition of membrane preparation (protein concentration of 5 ug/well for human CB2) into wells of a deep well plate containing [3H]CP 55,940 (120 Ci/mmol, a nonselective CB agonist commercially available from Tocris) in assay buffer (50 mM Tris, 2.5 mM EDTA, 5 mM MgCl2, and 0.5 mg/mL fatty acid free BSA, pH 7.4). After 90 min incubation at 30 C., binding reaction was terminated by the addition of 300 uL/well of cold assay buffer.		65	ALA3638858	cell-based format	BindingDB Database
4.	ALA658675	Binding	Binding affinity for Cannabinoid receptor 2		3	ALA1130181	single protein format	Scientific Literature
5.	ALA658833	Binding	Binding affinity for Cannabinoid receptor 2; ND=not determined		1	ALA1130181	single protein format	Scientific Literature
6.	ALA658834	Binding	Displacement of [3H]CP-55940 from rat forebrain membrane which expresses Cannabinoid receptor 2 in the presence of PMSF		16	ALA1135549	assay format	Scientific Literature
7.	ALA904763	Functional	Agonist activity at rat CB2 receptor	Rattus norvegicus	1	ALA1143658	assay format	Scientific Literature
8.	ALA1071856	Functional	Agonist activity at rat brain CB2 receptor by GTPgammaS binding assay	Rattus norvegicus	24	ALA1153132	assay format	Scientific Literature
9.	ALA1101970	Binding	Binding affinity to rat brain CB2 receptor	Rattus norvegicus	2	ALA1154937	assay format	Scientific Literature
10.	ALA1106560	Binding	Inhibition of rat cannabinoid CB2 receptor	Rattus norvegicus	1	ALA1157794	single protein format	Scientific Literature
11.	ALA1212388	Binding	Binding affinity to CB2 receptor in rat spleen homogenates at 1 uM	Rattus norvegicus	11	ALA1208718	tissue-based format	Scientific Literature
12.	ALA1763804	Functional	Agonist activity at rat CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level	Rattus norvegicus	2	ALA1759910	cell-based format	Scientific Literature
13.	ALA1763805	Functional	Agonist activity at rat CB2 receptor expressed in CHO-K1 cells assessed as inhibition of forskolin-stimulated cAMP level relative to control	Rattus norvegicus	2	ALA1759910	cell-based format	Scientific Literature
14.	ALA1767782	Functional	Agonist activity at rat CB2 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay	Rattus norvegicus	2	ALA1765069	cell-based format	Scientific Literature
15.	ALA1767898	Functional	Agonist activity at rat CB2 receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced cAMP production treated 15 mins before forskolin challenge measured after 1 hr by HTRF assay relative to control	Rattus norvegicus	2	ALA1765069	cell-based format	Scientific Literature
16.	ALA4356530	Binding	Displacement of [3H]-CP55940 from recombinant rat CB2 receptor expressed in CHOK1 cells assessed as residual binding level at 50 nM by radiometric assay relative to control	Rattus norvegicus	1	ALA4354821	cell-based format	Scientific Literature
17.	ALA4356531	Binding	Displacement of [3H]-CP55940 from recombinant rat CB2 receptor expressed in CHOK1 cells assessed as residual binding level at 500 nM by radiometric assay relative to control	Rattus norvegicus	1	ALA4354821	cell-based format	Scientific Literature
18.	ALA4356532	Binding	Displacement of [3H]-CP55940 from recombinant rat CB2 receptor expressed in CHOK1 cells assessed as residual binding level at 1000 nM by radiometric assay relative to control	Rattus norvegicus	1	ALA4354821	cell-based format	Scientific Literature
19.	ALA3413756	Binding	Binding affinity to CB2 receptor in rat spleen at 0.4 nM after 15 mins by autoradiography in presence of GW405833	Rattus norvegicus	1	ALA3407340	tissue-based format	Scientific Literature
20.	ALA4360724	Binding	Displacement of [3H]JWH-018 from CB2R in Wistar rat brain membranes at 10 uM after 60 mins by liquid scintillation analysis relative to control	Rattus norvegicus	1	ALA4359826	cell membrane format	Scientific Literature