#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706206	Binding	Enzymatic Assays: Type 2 DHODH activity was monitored with either the direct assay measuring the formation of orotate or via a chromogen reduction assay using DCIP. Although the extinction coefficient and absorption wavelength are preferable for the chromogen reduction assay, the inorganic electron acceptor DCIP can be utilized by DHODH as the final electron acceptor in lieu of CoQn when the endogenous substrate is at a low concentration. As such, the direct assay was used to measure the binding affinity of pfDHODH to CoQD and L-DHO. The enzymatic reaction was performed by varying the L-DHO concentration (1-400 μM) or CoQD concentration (1-200 μM) while keeping the other substrate constant and in excess. Inhibition of DHODH by small molecules was evaluated using the chromogen based assay with a final substrate concentration of 200 μM L-DHO, 18 μM CoQD, and 100 μM DCIP, unless otherwise noted.	Plasmodium falciparum	95	ALA3639068	single protein format	BindingDB Database
2.	ALA3820729	Binding	Inhibition of Plasmodium falciparum DHODH	Plasmodium falciparum	2	ALA3817764	single protein format	Scientific Literature
3.	ALA863916	Binding	Binding affinity to Plasmodium falciparum DHODH	Plasmodium falciparum	6	ALA1148537	single protein format	Scientific Literature
4.	ALA853733	Binding	Inhibition of recombinant Plasmodium falciparum DHODH at 10 uM	Plasmodium falciparum	6	ALA1139229	single protein format	Scientific Literature
5.	ALA911102	Binding	Inhibition of recombinant Plasmodium falciparum DHODH	Plasmodium falciparum	15	ALA1139229	single protein format	Scientific Literature
6.	ALA1065906	Binding	Inhibition of Plasmodium falciparum DHODH at 50 uM	Plasmodium falciparum	23	ALA1159041	single protein format	Scientific Literature
7.	ALA1220217	Binding	Inhibition of Plasmodium falciparum dihydroorotate dehydrogenase at 10 uM	Plasmodium falciparum	1	ALA1212705	single protein format	Scientific Literature
8.	ALA1785541	Binding	Inhibition of His6-tagged recombinant Plasmodium falciparum dihydroorotate dehydrogenase expressed in Escherichia coli using L-dihydroorotate as substrate after 10 to 15 mins by DCIP dye-based assay	Plasmodium falciparum	59	ALA1781999	assay format	Scientific Literature
9.	ALA3413104	Binding	Inhibition of Plasmodium falciparum DHODH using dihydroorotate substrate by DCIP assay	Plasmodium falciparum	54	ALA3407421	single protein format	Scientific Literature
10.	ALA942569	Binding	Inhibition of Plasmodium falciparum DHOD	Plasmodium falciparum	8	ALA1143267	single protein format	Scientific Literature
11.	ALA984851	Binding	Inhibition of ubiquinone binding site of Plasmodium falciparum dihydroorotate dehydrogenase	Plasmodium falciparum	17	ALA1138463	single protein format	Scientific Literature
12.	ALA961431	Binding	Inhibition of Plasmodium falciparum DHODH assessed as reduction of 2,6-dichloroindophenol by stopped flow spectroscopy	Plasmodium falciparum	14	ALA1150779	single protein format	Scientific Literature
13.	ALA961440	Binding	Inhibition of Plasmodium falciparum DHODH assessed as reduction of 2,6-dichloroindophenol by competitive binding assay	Plasmodium falciparum	1	ALA1150779	single protein format	Scientific Literature
14.	ALA1016154	Binding	Inhibition of Plasmodium falciparum DHODH	Plasmodium falciparum	40	ALA1153392	single protein format	Scientific Literature
15.	ALA1961096	Binding	Inhibition of Plasmodium falciparum C-terminal hexa-His tagged DHODH expressed in Escherichia coli by DCIP reduction assay	Plasmodium falciparum	83	ALA1955725	assay format	Scientific Literature
16.	ALA2014776	Binding	Inhibition of Plasmodium falciparum DHODH after 20 mins by 2,6-dichloroindophenol-reduction based assay	Plasmodium falciparum	41	ALA2010768	single protein format	Scientific Literature
17.	ALA2038351	Binding	Inhibition of Plasmodium falciparum DHODH-mediated 2,6-dichloroindophenol reduction after 20 mins by spectrophotometry	Plasmodium falciparum	1	ALA2034876	single protein format	Scientific Literature
18.	ALA2050309	Binding	Inhibition of Plasmodium falciparum DHODH	Plasmodium falciparum	6	ALA2046359	single protein format	Scientific Literature
19.	ALA2175115	Binding	Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli DH5alpha using 2,6-dichoroindophenol substrate by spectrophotometric analysis	Plasmodium falciparum	13	ALA2169788	assay format	Scientific Literature
20.	ALA2176172	Binding	Inhibition of Plasmodium falciparum DHOD after 5 to 10 mins by spectrophotometry	Plasmodium falciparum	4	ALA2169740	single protein format	Scientific Literature