| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA911225 | B | Inhibition of Saccharomyces cerevisiae GCN5 at 0.6 mM | Saccharomyces cerevisiae | 7 | single protein format | Scientific Literature | ||
| 2. | ALA911231 | F | Inhibition of GCN5-dependent HIS3-LacZ reporter gene transcription measured as level of beta-galactosidase activity in Saccharomyces cerevisiae | Saccharomyces cerevisiae | 3 | assay format | Scientific Literature | ||
| 3. | ALA911232 | F | Inhibition of GCN5-dependent HIS3-LacZ reporter gene transcription measured as level of beta-galactosidase activity in Saccharomyces cerevisiae in presence of 3-aminotriazole | Saccharomyces cerevisiae | 3 | assay format | Scientific Literature | ||
| 4. | ALA915500 | B | Inhibition of Saccharomyces cerevisiae recombinant GCN5 assessed as histone H3 acetylation | Saccharomyces cerevisiae | 2 | single protein format | Scientific Literature | ||
| 5. | ALA3419559 | B | Inhibition of yeast histone acetyltransferase Gcn5-Ada2-Ada3 complex using tetramer and [3H]-acetyl-CoA assessed as acetate incorporation after 30 mins by liquid scintillation counting | Saccharomyces cerevisiae | 22 | single protein format | Scientific Literature | ||
| 6. | ALA3419562 | B | Inhibition of yeast histone acetyltransferase Gcn5-Ada2-Ada3 complex using tetramer and [3H]-acetyl-CoA assessed as acetate incorporation after 30 mins by liquid scintillation counting in presence of 1 mM DTT | Saccharomyces cerevisiae | 22 | single protein format | Scientific Literature | ||
| 7. | ALA4153627 | B | Inhibition of yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins followed by biotin tagged H3 peptide ( 1 to 23 residues) and [3H]-Ac-CoA addition and measured after 60 mins by radioactive acetylation assay | Saccharomyces cerevisiae | 13 | assay format | Scientific Literature | ||
| 8. | ALA4153628 | B | Binding affinity to yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) by SPR analysis | Saccharomyces cerevisiae | 2 | assay format | Scientific Literature | ||
| 9. | ALA4153629 | B | Inhibition of yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) at 33 uM preincubated for 15 mins followed by biotin tagged H3 peptide ( 1 to 23 residues) and [3H]-Ac-CoA addition and measured after 60 mins by radioactive acetylation assay | Saccharomyces cerevisiae | 5 | assay format | Scientific Literature | ||
| 10. | ALA4153655 | B | Inhibition of yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) at 100 uM using biotin tagged H3 peptide ( 1 to 23 residues) as substrate preincubated for 30 mins followed by AC-CoA and substrate addition and measured after 30 mins by AlphaScreen assay | Saccharomyces cerevisiae | 8 | assay format | Scientific Literature | ||
| 11. | ALA4153656 | B | Binding affinity to yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) at 200 uM by CPMG NMR spectroscopy | Saccharomyces cerevisiae | 1 | assay format | Scientific Literature | ||
| 12. | ALA4153657 | B | Binding affinity to yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) at 200 uM by STD NMR spectroscopy | Saccharomyces cerevisiae | 1 | assay format | Scientific Literature |
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