# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1912161 | B | Displacement of [125I]adrenomedullin form CGRP receptor in human SK-N-MC cell membrane by competitive binding assay | Homo sapiens | 21 | cell-based format | Scientific Literature | ||
2. | ALA1912162 | B | Inhibition of human CGRP receptor expressed in huamn HEK293 cells coexpressing CLR/RAMP1 assessed as inhibition of CGRP-stimulated cAMP production after 1 hr | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
3. | ALA1912163 | B | Inhibition of CGRP receptor in human SK-N-MC cells assessed as inhibition of CGRP-stimulated cAMP production after 1 hr | Homo sapiens | 18 | cell-based format | Scientific Literature | ||
4. | ALA1912348 | B | Displacement of [125I]adrenomedullin form CGRP receptor in human SK-N-MC cell membrane by competitive binding assay in presence of 5 mM MgCl2 | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
5. | ALA1912357 | B | Displacement of [125I]adrenomedullin form CGRP receptor in human SK-N-MC cell membrane by competitive binding assay in the absence of MgCl2 | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
6. | ALA1912358 | B | Ratio Ki for CGRP receptor in human SK-N-MC cells in the absence of MgCl2 to Ki for CGRP receptor in human SK-N-MC cells in the presence of MgCl2 | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
7. | ALA954950 | F | Antagonist activity at human CGRP1 receptor in human SK-N-MC cells assessed as effect on human alpha-CGRP-induced cAMP accumulation | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
8. | ALA954951 | F | Agonist activity at human CGRP1 receptor in human SK-N-MC cells | Homo sapiens | 32 | cell-based format | Scientific Literature | ||
9. | ALA1291030 | B | Displacement of [125I]-CGRP from CGRP1 at 3 uM | 1 | single protein format | Scientific Literature | |||
10. | ALA3705382 | B | Binding Assay: Cells expressing recombinant human CL receptor/RAMP1 were washed with PBS and harvested in harvest buffer containing 50 mM HEPES, 1 mM EDTA and Complete protease inhibitors (Roche). The cell suspension was disrupted with a laboratory homogenizer and centrifuged at 48,000 g to isolate membranes. The pellets were resuspended in harvest buffer plus 250 mM sucrose and stored at −70C. For binding assays, 20 μg of membranes were incubated in 1 ml binding buffer (10 mM HEPES, pH 7.4, 5 mM MgCl2, and 0.2% BSA) for 3 hours at room temperature containing 10 pM 125I-hCGRP (GE Healthcare) and antagonist. The assay was terminated by filtration through 96-well GFB glass fiber filter plates (PerkinElmer) that had been blocked with 0.05% polyethyleneimine. The filters were washed 3 times with ice-cold assay buffer (10 mM HEPES, pH 7.4 and 5 mM MgCl2). Scintillation fluid was added and the plates were counted on a Topeount (Packard). | Homo sapiens | 20 | assay format | BindingDB Database | ||
11. | ALA3773899 | B | Displacement of [125I]CGRP from alpha CGRP in human SK-N-MC cells after 2 hrs by scintillation counter | Homo sapiens | 44 | cell-based format | Scientific Literature | ||
12. | ALA3773900 | B | Antagonist activity at CGRP (unknown origin) | Homo sapiens | 9 | single protein format | Scientific Literature | ||
13. | ALA3778190 | B | Displacement of [125I]hCGRPalpha from human recombinant CGRP1 receptor expressed in CHO cells at 10'-5 M | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
14. | ALA3779467 | B | Binding affinity to CGRP1 (unknown origin) at 10 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
15. | ALA4312729 | B | Activity at human CGRP1 at 10 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
16. | ALA5242054 | B | Inhibition of CGRP (unknown origin) | Homo sapiens | 3 | single protein format | Scientific Literature |