#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA939649	B	Reversal of ERK1 phosphorylation in mouse C2C12 myoblast at 20 uM in presence of 50 uM LY-294002 after 48 hrs	Mus musculus	1		single protein format	Scientific Literature
2.	ALA2345862	B	Activation of ERK1 in mouse RAW264.7 cells assessed as phosphorylation at Thr202/Tyr204 at 50 uM after 30 mins by Western blotting analysis	Mus musculus	1		cell-based format	Scientific Literature
3.	ALA3111976	B	Inhibition of IFNgamma-induced ERK phosphorylation in mouse RAW264.7 cells at 20 uM by Western blotting analysis relative to untreated control	Mus musculus	1		cell-based format	Scientific Literature
4.	ALA3111981	B	Inhibition of LPS-induced ERK phosphorylation in mouse RAW264.7 cells at 20 uM by Western blotting analysis	Mus musculus	1		cell-based format	Scientific Literature
5.	ALA3591271	B	Binding affinity to ERK1 in mouse fibroblasts assessed as half-life	Mus musculus	1		single protein format	Scientific Literature
6.	ALA3705763	B	Temperature Dependence Fluorescence Assay (TdF): The SAR (Structure Activity Relationship) for ERK ligands covered by this invention was interrogated using the TdF (Temperature Dependence Fluorescence) assay or best known as thermal shift assay [1]. The TdF assay was mainly conducted in the 96-well based CHROMO-4 real time fluorescence plate reader (BioRad). The Sypro Orange (Sigma-Aldrich), environmentally sensitive fluorescence dye, was used to monitor the protein folding-unfolding transition. Protein-ligand binding was gauged by the change (or shift) in the unfolding transition temperature (DTm) acquired at protein alone with respect to protein in the presence of ligand of interest.Compound of interest was first prepared in DMSO stock (typical concentration: 10 mM). Sample of 20 uL was then added into the 96-well PCR plate, where it consisted of 3 uM ERK protein and 15, 50 or 100 uM compound (depending on compound's solubility) in buffer (25 mM HEPES, 150 mM NaCl, pH=7.5 and 1 mM DTT).		90		single protein format	BindingDB Database
7.	ALA3862628	B	Inhibition of RANKL-induced ERK1 phosphorylation in mouse BMDM cells assessed as ratio of phosphorylated ERK1 to ERK1 at 10 uM preincubated for 5 mins followed RANKL/M-CSF addition for 5 mins by Western blot analysis (Rvb = 2.9 No_unit)	Mus musculus	3		cell-based format	Scientific Literature
8.	ALA4046556	B	Inhibition of ERK phosphorylation in mouse RAW264.7 cells incubated for 24 hrs followed by LPS-stimulation measured after 24 hrs by Western blot method	Mus musculus	1		cell-based format	Scientific Literature