#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA961254	B	Inhibition of Fms-mediated CSF1-stimulated mouse BMDM proliferation by bromodeoxyuridine incorporation assay	Mus musculus	29	ALA1140940	single protein format	Scientific Literature
2.	ALA2038874	B	Inhibition of Tel-fused FMS in mouse BA/F3 cells	Mus musculus	6	ALA2034853	cell-based format	Scientific Literature
3.	ALA2163157	B	Inhibition of FMS-mediated proliferation in CSF1-stimulated bone marrow-derived mouse macrophages assessed as inhibition of incorporation of bromodeoxyuridine into the DNA after 30 hrs by ELISA	Mus musculus	30	ALA2157860	single protein format	Scientific Literature
4.	ALA2213417	B	Inhibition of CSF1R in mouse M-NFS-60 cells assessed as inhibition of cell proliferation after 72 hrs by Celltiter-Blue assay	Mus musculus	17	ALA2203000	cell-based format	Scientific Literature
5.	ALA3088372	B	Inhibition of FMS in mouse BMDM cells	Mus musculus	57	ALA3085648	cell-based format	Scientific Literature
6.	ALA3705109	B	Fluorescence Polarization Competition Assay: A fluorescence polarization competition immunoassay was used to measure compound inhibition of CSF-1R phosphorylation of tyrosine on a synthetic CSF-1R555-568 peptide (SYEGNSYTFIDPTQ). The assay was performed in black 96-well microplates (Cat #42-000-0117, Molecular Devices, Sunnyvale, Calif.). To each well, 5 μL of compound (in 4% DMSO) were mixed with 2 μL of 3.5 nM CSF-1R, 25 mM MgCl2 in assay buffer (100 mM HEPES (hydroxyethylpiperazineethylsodiumsulfonate), pH 7.5, 1 mM DTT (dithiothreitol), 0.01% Tween-20), and 2 μL of 1540 μM peptide in assay buffer. The kinase reaction was initiated by adding 1 μL of 10 mM ATP in assay buffer. The final concentrations in the 10 uL reaction mixture were 100 mM HEPES, pH 7.5, 1 mM DTT, 0.01% Tween-20, 2% DMSO, 308 μM SYEGNSYTFIDPTQ, 1 mM ATP, 5 mM MgCl2, and 0.7 nM CSF-1R.	Mus musculus	85	ALA3638551	single protein format	BindingDB Database
7.	ALA3705295	B	Fluorescence Polarization Competition Assay: An autophosphorylation, fluroescence polarization competition immunoassay was used to determine the potency for c-fms inhibition.	Mus musculus	27	ALA3639032	single protein format	BindingDB Database
8.	ALA3705422	B	Fluorescence Polarization Competition Immunoassay: An autophosphorylation, fluorescence polarization competition immunoassay was used to determine the potency for c-fms inhibition exhibited by selected compounds of Formula I. The assay was performed in black 96-well microplates (LJL BioSystems). The assay buffer used was 100 mM 4-(2-hydroxyethyl)piperazine 1-ethanesulfonic acid (HEPES), pH 7.5, 1 mM 1,4-dithio-DL-threitol (DTT), 0.01% (v/v) Tween-20. Compounds were diluted in assay buffer containing 4% dimethylsulfoxide (DMSO) just prior to the assay. To each well, 5 μL of compound were added followed by the addition of 3 μL of a mix containing 33 nM c-fms (Johnson & Johnson PRD) and 16.7 mM MgCl2 (Sigma) in assay buffer. The kinase reaction was initiated by adding 2 μL of 5 mM ATP (Sigma) in assay buffer. The final concentrations in the assay were 10 nM c-fms, 1 mM ATP, 5 mM MgCl2, 2% DMSO.	Mus musculus	2	ALA3638963	single protein format	BindingDB Database
9.	ALA3888751	B	Fluorescence Polarization Competition Immunoassay: An autophosphorylation, fluorescence polarization competition immunoassay was used to determine the potency for c-fms inhibition exhibited by selected compounds of Formula I. The assay was performed in black 96-well microplates (LJL BioSystems). The assay buffer used was 100 mM 4-(2-hydroxyethyl)piperazine 1-ethanesulfonic acid (HEPES), pH 7.5, 1 mM 1,4-dithio-DL-threitol (DTT), 0.01% (v/v) Tween-20. Compounds were diluted in assay buffer containing 4% dimethylsulfoxide (DMSO) just prior to the assay. To each well, 5 μL of compound were added followed by the addition of 3 μL of a mix containing 33 nM c-fms (Johnson & Johnson PRD) and 16.7 mM MgCl2 (Sigma) in assay buffer. The kinase reaction was initiated by adding 2 μL of 5 mM ATP (Sigma) in assay buffer. The final concentrations in the assay were 10 nM c-fms, 1 mM ATP, 5 mM MgCl2, 2% DMSO. Control reactions were ran in each plate: in positive and negative control wells, assay buffer (made 4% in DMSO) was substituted for the compound; in addition, positive control wells received 1.2 μL of 50 mM ethylenediaminetetraaceticacid (EDTA). The plates were incubated at room temperature for 45 min. At the end of the incubation, the reaction was quenched with 1.2 μL of 50 mM EDTA (EDTA was not added to the positive control wells at this point; see above). Following a 5-min incubation, each well received 10 μL of a 1:1:3 mixture of anti-phosphotyrosine antibody, 10×, PTK green tracer, 10× (vortexed), FP dilution buffer, respectively (all from PanVera, cat. #P2837). The plate was covered, incubated for 30 min at room temperature and the fluorescence polarization was read on the Analyst. The instrument settings were: 485 nm excitation filter; 530 nm emission filter; Z height: middle of well; G factor: 0.93. Under these conditions, the fluorescence polarization values for positive and negative controls were approximately 300 and 150, respectively, and were used to define the 100% and 0% inhibition of the c-fms reaction.	Mus musculus	44	ALA3886820	single protein format	BindingDB Database
10.	ALA3888766	B	Fluorescence Polarization Competition Immunoassay: An autophosphorylation, fluorescence polarization competition immunoassay was used to determine the potency for c-fms inhibition exhibited by selected compounds of Formula I. The assay was performed in black 96-well microplates (LJL BioSystems). The assay buffer used was 100 mM 4-(2-hydroxyethyl)piperazine 1-ethanesulfonic acid (HEPES), pH 7.5, 1 mM 1,4-dithio-DL-threitol (DTT), 0.01% (v/v) Tween-20. Compounds were diluted in assay buffer containing 4% dimethylsulfoxide (DMSO) just prior to the assay. To each well, 5 μL of compound were added followed by the addition of 3 μL of a mix containing 33 nM c-fms (Johnson & Johnson PRD) and 16.7 mM MgCl2 (Sigma) in assay buffer. The kinase reaction was initiated by adding 2 μL of 5 mM ATP (Sigma) in assay buffer. The final concentrations in the assay were 10 nM c-fms, 1 mM ATP, 5 mM MgCl2, 2% DMSO. Control reactions were ran in each plate: in positive and negative control wells, assay buffer (made 4% in DMSO) was substituted for the compound; in addition, positive control wells received 1.2 μL of 50 mM ethylenediaminetetraaceticacid (EDTA). The plates were incubated at room temperature for 45 min. At the end of the incubation, the reaction was quenched with 1.2 μL of 50 mM EDTA (EDTA was not added to the positive control wells at this point; see above). Following a 5-min incubation, each well received 10 μL of a 1:1:3 mixture of anti-phosphotyrosine antibody, 10×, PTK green tracer, 10× (vortexed), FP dilution buffer, respectively (all from PanVera, cat. # P2837). The plate was covered, incubated for 30 min at room temperature and the fluorescence polarization was read on the Analyst. The instrument settings were: 485 nm excitation filter; 530 nm emission filter; Z height: middle of well; G factor: 0.93. Under these conditions, the fluorescence polarization values for positive and negative controls were approximately 300 and 150, respectively, and were used to define the 100% and 0% inhibition of the c-fms reaction.	Mus musculus	10	ALA3886829	single protein format	BindingDB Database
11.	ALA4038041	B	Inhibition of CSF1R phosphorylation in mouse RAW264.7 cells preincubated for 6 hrs followed by CSF1 stimulation for 20 mins by Western blot analysis	Mus musculus	1	ALA4033795	cell-based format	Scientific Literature
12.	ALA4038042	B	Inhibition of CSF1R in mouse RAW264.7 cells assessed as suppression of AKT phosphorylation preincubated for 6 hrs followed by CSF1 stimulation for 20 mins by Western blot analysis	Mus musculus	1	ALA4033795	cell-based format	Scientific Literature
13.	ALA4038043	B	Inhibition of CSF1R phosphorylation in mouse RAW264.7 cells at 80 nM preincubated for 6 hrs followed by CSF1 stimulation for 20 mins by Western blot analysis relative to control	Mus musculus	1	ALA4033795	cell-based format	Scientific Literature
14.	ALA4038044	B	Inhibition of CSF1R phosphorylation in mouse RAW264.7 cells assessed as reduction in AKT phosphorylation at 80 nM preincubated for 6 hrs followed by CSF1 stimulation for 20 mins by Western blot analysis relative to control	Mus musculus	1	ALA4033795	cell-based format	Scientific Literature
15.	ALA4256961	B	Inhibition of c-fms in mouse bone marrow macrophages assessed as reduction in recombinant mouse CSF-1-driven cell proliferation by measuring bromodeoxyuridine incorporation incubated for 30 hrs by ELISA	Mus musculus	74	ALA4256944	single protein format	Patent Bioactivity Data
16.	ALA4256964	B	Inhibition of c-FMS in mouse BMDM assessed as reduction in CSF-1 stimulated cell proliferation by measuring BrDU incorporation treated for 30 hrs followed by BrDU addition during last 6 hrs of incubation by ELISA	Mus musculus	2	ALA4256945	single protein format	Patent Bioactivity Data
17.	ALA4325647	B	Inhibition of CSF1R in CSF1-stimulated mouse RAW264.7 cells assessed as reduction in CSF1R phosphorylation preincubated for 6 hrs followed by CSF1 stimulation and measured after 20 mins by Western blot analysis	Mus musculus	1	ALA4321879	cell-based format	Scientific Literature
18.	ALA4325648	B	Inhibition of CSF1R in CSF1-stimulated mouse RAW264.7 cells assessed as reduction in AKT phosphorylation preincubated for 6 hrs followed by CSF1 stimulation and measured after 20 mins by Western blot analysis	Mus musculus	1	ALA4321879	cell-based format	Scientific Literature
19.	ALA4361187	B	Inhibition of CSF1R in mouse BAF3(transformed) cells assessed as growth inhibition after 72 hrs by CCK8 assay	Mus musculus	1	ALA4359837	assay format	Scientific Literature
20.	ALA4424849	B	Inhibition of CSF1R in CSF1-induced DBA/1J mouse BMMC assessed as reduction in LPS-induced IL6 production preincubated for overnight followed by LPS-stimulation and measured after 6 hrs by ELISA	Mus musculus	21	ALA4422686	single protein format	Scientific Literature