#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA984126	B	Binding affinity to human PARP3 PARP domain by isothermal titration colorimetry	Homo sapiens	4		single protein format	Scientific Literature
2.	ALA984128	B	Inhibition of human PARP3 auto-ADP ribosylation by Western blotting	Homo sapiens	1		single protein format	Scientific Literature
3.	ALA1060807	B	Inhibition of human PARP3 up to 500 nM	Homo sapiens	2		single protein format	Scientific Literature
4.	ALA1074655	B	Inhibition of PARP3		2		single protein format	Scientific Literature
5.	ALA1115412	B	Inhibition of human PARP3 by trichloroacetic acid precipitation assay	Homo sapiens	1		single protein format	Scientific Literature
6.	ALA2382485	B	Inhibition of PARP3 (unknown origin)	Homo sapiens	2		single protein format	Scientific Literature
7.	ALA2382493	B	Inhibition of PARP3 (unknown origin) at 10 uM relative to control	Homo sapiens	2		single protein format	Scientific Literature
8.	ALA2384337	B	Inhibition of PARP3 (unknown origin) using histone as substrate after 1 hr by luminescence assay	Homo sapiens	1		single protein format	Scientific Literature
9.	ALA3096299	B	Inhibition of full length ATRD3 (unknown origin)	Homo sapiens	14		single protein format	Scientific Literature
10.	ALA3096304	B	Binding affinity to ATRD3 (unknown origin) assessed as change in melting temperature by thermal stabilization assay	Homo sapiens	56		single protein format	Scientific Literature
11.	ALA3242397	B	Inhibition of recombinant human PARP-3 at 10 uM relative to control	Homo sapiens	1		single protein format	Scientific Literature
12.	ALA3382572	B	Inhibition of human recombinant PARP3 at 10 uM incubated for 1 hr using using NAD+, biotinylated NAD+ and activated DNA by luminescence based assay	Homo sapiens	1		single protein format	Scientific Literature
13.	ALA3382582	B	Inhibition of human recombinant PARP3 at 1 uM incubated for 1 hr using using NAD+, biotinylated NAD+ and activated DNA by luminescence based assay	Homo sapiens	1		single protein format	Scientific Literature
14.	ALA3595283	B	Inhibition of full-length human ARTD3 using 500 nM NAD substrate after 1 hrs incubation	Homo sapiens	4		single protein format	Scientific Literature
15.	ALA3707489	B	Displacement Assay: Affinity evaluation of the tested compounds and their selectivity with respect to the different PARP isoforms of interest was assessed in a displacement assay.The identification of compounds capable of binding several PARP proteins is carried out through a screening method including the steps of a) providing a reaction mixture containing: the PARP protein isoform under investigation,a compound of formula (IP). b) comparing the polarization signal generated in the absence of the test compound with the one generated in the presence of different concentrations of the test compound, and c) evaluating the ability of the test compound to displace the compound of formula (IP) as defined above indicated from a decreased fluorescence polarization level. Preferably, for the screening method above cited, both the PARP protein and the 5H-phenanthridin-6-one-derived probe of formula (IP) are pre-mixed, or the PARP protein and the test compound are pre-mixed.	Homo sapiens	3		single protein format	BindingDB Database
16.	ALA3743827	B	Inhibition of PARP3 (unknown origin) using activated DNA as substrate after 1 hr by streptavidin-horseradish peroxidase-based luminescence assay	Homo sapiens	3		single protein format	Scientific Literature
17.	ALA3757402	B	Inhibition of human ARTD3 by fluorescence analysis	Homo sapiens	2		single protein format	Scientific Literature
18.	ALA3887959	B	Enzyme Assay: In order to test the selectivity of substituents on piperazinotriazole ring within the PARP family, the selectivity of compound S3 and positive compound AZD2281 were tested.	Homo sapiens	2		single protein format	BindingDB Database
19.	ALA3887991	B	PARP-2 and PARP-3 Enzymatic Assay: PARP-2 and PARP-3 enzymatic assays were conducted using commercial PARP-2/PARP-3 Chemiluminescent Assay Kit (BPS Biosciences) and the protocols with the kits. Briefly, histones were coated in a high binding plate first, and incubated with PARP-2 or PARP-3, and increasing concentrations of compounds for 0.5 h. Then, biotinylated NAD and activated DNA were added to the wells. The biotinylated PARylation product was measured by adding streptavidin-HRP and HRP substrates which produce chemiluminescence.		10		single protein format	BindingDB Database
20.	ALA3888856	B	Biochemical Assay: The identification of compounds capable of binding several PARP proteins is carried out through a screening method including the steps of a) providing a reaction mixture containing: b) comparing the polarization signal generated in the absence of the test compound with the one generated in the presence of different concentrations of the test compound, and c) evaluating the ability of the test compound to displace the compound of formula (IP) as defined above indicated from a decreased fluorescence polarization level.The polarization signal can be measured, e.g., by a plate reader such as the Saphire2 (Tecan).	Homo sapiens	13		single protein format	BindingDB Database