# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1827004 | B | Activity of WEE2 kinase at 1 uM | 1 | single protein format | Scientific Literature | |||
2. | ALA1908443 | B | Binding constant for WEE2 kinase domain | 72 | single protein format | Scientific Literature | |||
3. | ALA995070 | B | Inhibition of WEE2 at 3 uM | 1 | single protein format | Scientific Literature | |||
4. | ALA1059399 | B | Inhibition of WEE2 assessed as enzyme activity at 1 uM relative to untreated control | 1 | single protein format | Scientific Literature | |||
5. | ALA1168308 | B | Inhibition of WEE2 at 1 uM | 6 | single protein format | Scientific Literature | |||
6. | ALA1175120 | B | Inhibition of WEE2 at 10 uM | 2 | single protein format | Scientific Literature | |||
7. | ALA1245024 | B | Binding affinity to WEE2 | 7 | single protein format | Scientific Literature | |||
8. | ALA2209235 | B | Inhibition of WEE2 assessed as residual activity at 10 uM relative to control | 1 | single protein format | Scientific Literature | |||
9. | ALA3876698 | B | Inhibition of human WEE2 (M204 to K492 residues) expressed in bacterial expression system at 1 uM by KINOMEScan assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
10. | ALA4018852 | B | Binding affinity to recombinant human full-length N-terminal His8-tagged Wee2 (1 to 567 residues) expressed in human Expi293F cells assessed as dessociation constant by quantitative real-time PCR method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
11. | ALA4018868 | B | Binding affinity to recombinant human N-terminal His6-tagged Wee2 kinase domain (202 to 492 residues) expressed in Escherichia coli BL21 (DE3) by isothermal titration calorimetry | Homo sapiens | 9 | assay format | Scientific Literature | ||
12. | ALA4018876 | B | Binding affinity to recombinant human N-terminal His6-tagged Wee2 kinase domain (202 to 492 residues) expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature at 50 uM by differential scanning fluorimetry | Homo sapiens | 9 | assay format | Scientific Literature | ||
13. | ALA4025437 | B | Inhibition of wild-type human partial length WEE2 (M204 to K492 residues) expressed in bacterial expression system assessed as residual activity at 1 uM by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
14. | ALA4044248 | B | Inhibition of wild-type human partial length WEE2 (M204 to K492 residues) expressed in Escherichia coli BL21 assessed as residual activity at 1000 nM after 30 mins by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
15. | ALA4054996 | B | Inhibition of wild-type human partial length WEE1B (M204 to K492 residues) expressed in bacterial expression system assessed as residual activity at 50 nM by Kinomescan method relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
16. | ALA4157327 | B | Binding affinity to wild-type human partial length WEE2 (M204 to K492 residues) expressed in bacterial expression system assessed as residual binding at 1 uM by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
17. | ALA4186106 | B | Binding affinity to wild-type human partial length WEE2 (M204 to K492 residues) expressed in Escherichia coli BL21 at 1 uM by Kinomescan method relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
18. | ALA4191233 | B | Inhibition of recombinant full-length human Wee1B at 1 uM using LSNLYHQGKFLQTFCGSPLYRRR as substrate after 120 mins in presence of [gamma-33P]ATP by scintillation counting method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
19. | ALA4231006 | B | Binding affinity to wild-type human partial length WEE2 (M204 to K492 residues) expressed in bacterial expression system assessed as residual binding level at 1 uM by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
20. | ALA4264595 | B | Binding affinity to wild-type human partial length WEE2 (M204 to K492 residues) expressed in bacterial expression system assessed as remaining unbound target protein level at 1000 nM by Kinomescan method relative to control | Homo sapiens | 1 | assay format | Scientific Literature |