Inhibition of Clostridium botulinum strain Hall A hyper botulinum neurotoxin type A1 in rat spinal cord cells assessed as intact SNAP25 protein level at 200 uM after 24 hrs by Western blot using monoclonal anti-SNAP25 antibody
Inhibition of Clostridium botulinum strain Hall A hyper botulinum neurotoxin type A1 in rat spinal cord cells assessed as intact SNAP25 protein level at 1 mM after 24 hrs by Western blot using monoclonal anti-SNAP25 antibody
Inhibition of Clostridium botulinum Hall BoNT/A-induced toxicity in mouse Neuro2a cells assessed as inhibition of SNAP25 substrate cleavage at 60 uM by Western blotting
Inhibition of Clostridium botulinum Hall BoNT/A-induced toxicity in mouse Neuro2a cells assessed as inhibition of SNAP25 substrate cleavage at 45 uM by Western blotting
Inhibition of Clostridium botulinum Hall BoNT/A-induced toxicity in mouse Neuro2a cells assessed as inhibition of SNAP25 substrate cleavage at 30 uM by Western blotting
Inhibition of Clostridium botulinum Hall BoNT/A-induced toxicity in mouse Neuro2a cells assessed as inhibition of SNAP25 substrate cleavage at 15 uM by Western blotting
Inhibition of Clostridium botulinum Hall BoNT/A-induced paralysis in mouse phrenic nerve hemidiaphragm assessed as time required for loss of 50% twitch tension at 2 uM
Inhibition of Clostridium botulinum Hall BoNT/A-induced paralysis in mouse phrenic nerve hemidiaphragm assessed as delay in paralytic half-time at 2 uM relative to control
Inhibition of Clostridium botulinum Hall BoNT/A protease activity using Ac-SNKTRIDEANQRATKML-NH2 as substrate at 100 uM after 5 mins by RP-HPLC analysis
Inhibition of Clostridium botulinum Hall BoNT/A protease activity using Ac-SNKTRIDEANQRATKML-NH2 as substrate at 20 uM after 5 mins by RP-HPLC analysis
Inhibition of Clostridium botulinum Hall BoNT/A-mediated paralysis in CD1 mouse phrenic nerve hemidiaphragm assessed as time required to 50% loss of twitch tension at 20 uM measured upto 5 hrs (Rvb = 62 mins)
Inhibition of Clostridium botulinum Hall BoNT/A-mediated paralysis in CD1 mouse phrenic nerve hemidiaphragm assessed as time required to 50% loss of twitch tension at 2 uM measured upto 5 hrs (Rvb = 62 mins)
PubChem BioAssay. High-throughput multiplex microsphere dose response for inhibitors of toxin protease, specifically Botulinum neurotoxin light chain A protease, compounds from Powder Set 01. (Class of assay: confirmatory)