PubChem BioAssay. Counterscreen for inhibitors of the interaction of the lipase co-activator protein, abhydrolase domain containing 5 (ABHD5) with perilipin-5 (MLDP; PLIN5): Luminescence-based biochemical high throughput dose response assay to identify inhibitors of Hepatocyte nuclear factor 4 (HNF4) dimerization. (Class of assay: confirmatory)
Increase in transcriptional activity of HNF-4alpha ligand binding domain expressed in human Hep G2 cells co-transfected with Gal4-DBD by luciferase reporter gene assay
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity after 16 hrs by beta-galactosidase one hybrid assay
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity at 9.3 uM by beta-galactosidase one hybrid assay relative to control
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity at 12.5 uM by beta-galactosidase one hybrid assay relative to control
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity at 18.7 uM by beta-galactosidase one hybrid assay relative to control
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity at 6.2 uM by beta-galactosidase one hybrid assay relative to control
Agonist activity at human full length HNF4alpha nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity at 100 uM by beta-galactosidase one hybrid assay relative to control
Agonist activity at AF1 deleted human HNF4alpha7 nuclear receptor expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity after 16 hrs by beta-galactosidase one hybrid assay
Agonist activity at human HNF4alpha7 nuclear receptor LBDdetaF expressed in human HepG2/C3A cells co-transfected with LexADBD assessed as increase in transcriptional activity after 16 hrs by beta-galactosidase one hybrid assay
Stabilization of thermal denaturation-induced conformational change in human HNF4alpha nuclear receptor ligand binding domain assessed as change in transition temperature by thermofluor assay
Activation of HNFalpha transcriptional activity in human HepG3/C3A cells transfected with ApoB-luc assessed as activation of luciferase reporter gene after 24 hrs
Activation of HNFalpha transcriptional activity in human HepG3/C3A cells transfected with ApoCIII-luc assessed as activation of luciferase reporter gene after 24 hrs
Displacement of radiolabeled methyl-4-[5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-3-ethyloxy-2-naphthalenyl]benzoyl benzoate from HNF4alpha (unknown origin)
Displacement of [3H]methyl4-[5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-3-ethyloxy-2-naphthalenyl]benzoyl benzoate from HNF4alpha (unknown origin) by radioligand binding assay
Agonist activity at recombinant human pFA-CMV-fused HNF4alpha LBD expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid at 0.01 to 10 uM incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay
Agonist activity at recombinant human pFA-CMV-fused HNF4alpha LBD expressed in HEK293T cells transfected with pFR-luciferase plasmid and pRL-SV40 plasmid at 0.001 to 0.3 uM incubated for 14 to 16 hrs by dual glo luciferase reporter gene assay