Ratio IC50 for lactose to compound for galectin-9 binding to biotinylated neoglycoprotein with p-isothiocyanatophynyl lactoside assessed as lectin-neoglycoprotein complex formation after 1 hr by spectrophotometrically relative to lactose
Binding affinity at recombinant human N-terminal Galectin 9 expressed in Escherichia coli BL21 at 2 mM incubated for 5 mins by fluorescence anisotropy assay
Binding affinity at recombinant human C-terminal Galectin 9 expressed in Escherichia coli BL21 at 2 mM incubated for 5 mins by fluorescence anisotropy assay
Displacement of (3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-yl]-3'-(3,5-di-methoxybenzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside) fluorescent probe from human galectin-9 N-terminal domain expressed in Escherichia coli BL21 Star (DE3) by competitive fluorescence polarization assay
Displacement of (3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-yl]-3'-(3,5-di-methoxybenzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside) fluorescent probe from human galectin-9 C-terminal domain expressed in Escherichia coli BL21 Star (DE3) by competitive fluorescence polarization assay
Competitive binding affinity to recombinant human galectin-9 N-terminal after 5 mins in presence of fluorescent probe 2-(fluorescein-5-yl-carbonylamino)ethyl-beta-D-galactopyranosyl(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl(1-3)-beta-D-galactopyranosyl(1-4)-beta-D-glucopyranoside by fluorescence polarization assay
Competitive binding affinity to recombinant human galectin-9 C-terminal after 5 mins in presence of fluorescent probe 3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-yl]-3'-(3,5-dimethoxy-benzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside by fluorescence polarization assay
Binding affinity to human galectin-9 N terminal domain assessed as dissociation constant measured up to 1500 uM by competitive fluorescence polarization assay
Binding affinity to human galectin-9 C terminal domain assessed as dissociation constant measured up to 1500 uM by competitive fluorescence polarization assay