# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1000314 | B | Inhibition of recombinant perforin-mediated lysis of human Jurkat T cells by 51Cr release assay | 59 | cell-based format | Scientific Literature | |||
2. | ALA1000319 | B | Inhibition of human KHYG-1 NK cells delivered perforin-mediated death of human K562 cells upto 20 uM preincubated for 20 mins with KHYG-1 cells measured after 30 mins by 51Cr release assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
3. | ALA1000321 | B | Inhibition of human CD3-CD56+ NK cell delivered perforin-mediated death of human K562 cells upto 20 uM preincubated for 30 mins with human CD3-CD56+ NK cell measured after 4 hrs by 51Cr release assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
4. | ALA1799368 | B | Inhibition of recombinant perforin mediated lysis of human Jurkat cells pre-incubated for 30 mins with perforin followed by incubation with Jurkat cells at 37 degC for 4 hrs in presence of 0.1% BSA by [51Cr] release assay | 38 | cell-based format | Scientific Literature | |||
5. | ALA1799369 | B | Inhibition of perforin released from human KHYG-1 NK cells assessed as inhibition of perforin mediated lysis of human K562 cells at 20 uM in presence of 0.1% BSA by [51Cr] release assay | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
6. | ALA1799370 | B | Inhibition of perforin released from human KHYG-1 NK cells assessed as inhibition of perforin mediated lysis of human K562 cells at 20 uM in presence of 10% mouse serum by [51Cr] release assay | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
7. | ALA1936847 | B | Inhibition of recombinant perforin-mediated lysis of Jurkat cells assessed as [51Cr] release preincubated with perforin for 30 mins followed by incubation with [51Cr]-labeled Jurkat cells by gamma counting | 43 | cell-based format | Scientific Literature | |||
8. | ALA1936848 | B | Inhibition of perforin released from human KHYG-1 NK cells assessed as inhibition of perforin-mediated lysis of human K562 cells at 20 uM by [51Cr] release assay in absence of serum | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
9. | ALA1936849 | B | Inhibition of perforin released from human KHYG-1 NK cells assessed as inhibition of perforin-mediated lysis of human K562 cells at 20 uM by [51Cr] release assay in presence of 10% mouse serum | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
10. | ALA1936997 | B | Inhibition of recombinant perforin-mediated lysis of Jurkat cells assessed as [51Cr] release at 20 uM preincubated with perforin for 30 mins followed by incubation with [51Cr]-labeled Jurkat cells by gamma counting | 1 | cell-based format | Scientific Literature | |||
11. | ALA3106780 | B | Inhibition of perforin-mediated lysis of human [51Cr]-labeled K562 cells assessed as release of [51Cr] at 5 uM preincubated for 20 mins with human KHYG-1 cells followed by addition of human [51Cr]-labeled K562 cells measured after 4 hrs by gamma counting analysis in presence of 10% mouse serum | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
12. | ALA3106781 | B | Inhibition of perforin-mediated lysis of human [51Cr]-labeled K562 cells assessed as release of [51Cr] at 5 uM preincubated for 20 mins with human KHYG-1 cells followed by addition of human [51Cr]-labeled K562 cells measured after 4 hrs by gamma counting analysis in absence of mouse serum | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
13. | ALA3106782 | B | Inhibition of perforin-mediated lysis of human [51Cr]-labeled K562 cells assessed as release of [51Cr] at 20 uM preincubated for 20 mins with human KHYG-1 cells followed by addition of human [51Cr]-labeled K562 cells measured after 4 hrs by gamma counting analysis in presence of 10% mouse serum | Homo sapiens | 10 | cell-based format | Scientific Literature | ||
14. | ALA3106783 | B | Inhibition of perforin-mediated lysis of human [51Cr]-labeled K562 cells assessed as release of [51Cr] at 20 uM preincubated for 20 mins with human KHYG-1 cells followed by addition of human [51Cr]-labeled K562 cells measured after 4 hrs by gamma counting analysis in absence of mouse serum | Homo sapiens | 10 | cell-based format | Scientific Literature | ||
15. | ALA3106784 | B | Inhibition of recombinant perforin (unknown origin)-mediated lysis of human [51Cr]-labelled Jurkat cells assessed as release of [51Cr] preincubated for 30 mins followed by addition of cells measured after 4 hrs by gamma counting analysis | Homo sapiens | 68 | cell-based format | Scientific Literature | ||
16. | ALA3106793 | B | Reversible binding affinity to wild type perforin (unknown origin) by SPR analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
17. | ALA3756437 | B | Inhibition of recombinant perforin (unknown origin) mediated lysis of human Jurkat cells pre-incubated for 30 mins with perforin followed by incubation with Jurkat cells at 37 degC for 4 hrs assessed as 51Cr release by gamma counting analysis | Homo sapiens | 46 | cell-based format | Scientific Literature | ||
18. | ALA3756438 | B | Inhibition of perforin released from human KHYG-1 NK cells assessed as inhibition of perforin mediated lysis of human K562 cells at 20 uM by measuring 51Cr release incubated for 20 mins by gamma counting analysis relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
19. | ALA4004266 | B | Inhibition of perforin in human KHYG-1 cells assessed as decrease in lysis of 51Cr-labelled human K562 cells by measuring 51Cr release at 20 uM preincubated for 30 mins followed by cell line addition measured after 4 hrs by gamma counting method relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
20. | ALA4004269 | B | Inhibition of recombinant perforin (unknown origin) assessed as decrease in lysis of 51Cr-labelled human Jurkat cells by measuring 51Cr release preincubated for 30 mins followed by cell line addition measured after 4 hrs in presence of 0.1% BSA by gamma counting method | Homo sapiens | 18 | assay format | Scientific Literature |