| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2013321 | B | Inhibition of human recombinant DDX3 helicase activity expressed in Escherichia coli assessed as conversion of a 6-FAM-labeled double stranded RNA in to single stranded nucleic acid by laser scanning densitometric analysis | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 2. | ALA2013324 | B | Inhibition of human recombinant DDX3 ATPase activity expressed in Escherichia coli assessed as [gamma-32P] ATP hydrolysis after 5 mins by densitometric analysis | Homo sapiens | 7 | assay format | Scientific Literature | ||
| 3. | ALA2013328 | B | Competitive inhibition of human recombinant DDX3 expressed in Escherichia coli using 25 nM double strand 18/36mer RNA substrate by PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA2013329 | B | Competitive inhibition of human recombinant DDX3 expressed in Escherichia coli using 50 nM double strand 18/36mer RNA substrate by PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA2013330 | B | Competitive inhibition of human recombinant DDX3 expressed in Escherichia coli using 100 nM double strand 18/36mer RNA substrate by PAGE analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA1003099 | B | Inhibition of ATP dependent RNA unwinding activity of human RNA helicase DDX3 | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 7. | ALA995080 | B | Inhibition of ATPase activity in human recombinant DDX3 expressed in Escherichia coli by luciferase-based luminescence assay in presence of ATP | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 8. | ALA3991688 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 243 | subcellular format | Kuster lab chemical proteomics drug profiling | ||
| 9. | ALA4044790 | B | Inhibition of DDX3 (unknown origin) preincubated for 10 mins followed by ATP addition by malachite green dye-based assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 10. | ALA4118955 | B | Binding affinity to DDX3X in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4481328 | B | Inhibition of full length His-tagged human DDX3X expressed in Escherichia coli assessed as reduction in DDX3X helicase activity using FAM-labeled double stranded RNA as substrate after 40 mins by FRET assay relative to control | Homo sapiens | 21 | assay format | Scientific Literature | ||
| 12. | ALA4481329 | B | Competitive inhibition of full length His-tagged human DDX3X expressed in Escherichia coli assessed as reduction in DDX3X helicase activity using 0.024 uM FAM-labeled double stranded RNA as substrate after 40 mins by FRET assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4481330 | B | Competitive inhibition of full length His-tagged human DDX3X expressed in Escherichia coli assessed as reduction in DDX3X helicase activity using 0.1 uM FAM-labeled double stranded RNA as substrate after 40 mins by FRET assay relative to control | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA4481331 | B | Competitive inhibition of human full length His-tagged DDX3X helicase activity expressed in Escherichia coli using FAM-labeled double stranded RNA as substrate at 0.1 uM after 40 mins by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA4481332 | A | Inhibition of human DDX3X ATPase activity using DDX3 as substrate by ADP-Glo kinase assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 16. | ALA4616148 | B | Inhibition of recombinant full length human His-tagged DDX3X expressed in Escherichia coli using dsRNA as substrate measured after 40 mins in presence of ATP by FRET assay | Homo sapiens | 24 | assay format | Scientific Literature | ||
| 17. | ALA4616153 | B | Induction of DDX3X recruitment in human HuH7 cells infected with 100 TCID50 of Dengue virus serotype 2 New Guinea C assessed as colocalization of DDX3X in perinuclear region at 20 uM measured at 6 to 24 hrs post-infection by Alexa fluor 488 dye based confocal microscopy | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA4616155 | B | Induction of DDX3X recruitment in human HuH7 cells infected with 100 TCID50 of Dengue virus serotype 2 New Guinea C assessed as increase in DDX3X expression in perinuclear region at 20 uM measured at 48 to 72 hrs post-infection by Alexa fluor 488 dye based confocal microscopy | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA4687213 | B | Inhibition of human DDX3X using dsRNA as substrate measured every 30 sec for 40 mins by FRET assay | Homo sapiens | 26 | single protein format | Scientific Literature | ||
| 20. | ALA5216450 | B | Binding affinity to DDX3X (unknown origin) assessed as inhibition constant | Homo sapiens | 31 | single protein format | Scientific Literature |
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