#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705653	Binding	Enzymatic Assay: The SCD1 enzymatic assay was done in a volume of 50 μL using 10 μg of RLM (prepared as described above) in a 96-well polypropylene plate (enzyme reaction buffer contains 0.1 M K-Phosphate Buffer, 10 mM ATP, 6 mM MgCl2. 1 mM CoA, 1 mM β-NADH, 1.6 mM L-glutathione, 20 μM Stearoyl-CoA). Stearoyl-[9,10-3H]-CoA (ARC-0390, 1 mCi/mL, 60 Ci/mmol,) was added at a final concentration of 2 μCi/mL. Test compound was then added to the reaction mixture at the selected concentration. After incubation at room temperature for 2 hours, 5 μL 1 N HCl was added to stop the reaction, followed by addition of 25 μL of 10% charcoal. The reaction mixture was then transferred to 96-well Multiscreen plate (Millipore, Cat#MSFCN6B50). [3H2O] was collected into Opti-plate (PE, Cat#6005290) by centrifuge, at 1,000 rpm for 2 minutes. 150 μL Microscint 40 (PE, cat #6013641) was then added to each well and counted on Topcount for [3H] counts per minute (cpm).		84	ALA3639165	single protein format	BindingDB Database
2.	ALA3705921	Binding	Enzyme Inhibition Assay: Human SCD-1 enzyme activity using HepG2 cell microsomes after treating with inhibitory compounds (% inhibition):Human hepatocarcinoma HepG2 cells (ATCC, HB-8065) are cultured to confluence and trypsinised. The cell pellet is taken up with 10 mM Tris (pH 7.4) sucrose (250 mM) DTT (1 mM) buffer and the cells are lysed by sonication. The microsomes are obtained after centrifugation at 10,000 g for 20 minutes at 4 C. followed by centrifugation of the supernatant at 100,000 g for 60 minutes at 4 C. The pellet is taken up with 10 mM Tris (pH 7.4) sucrose (250 mM) buffer at 4 C. and the microsomal proteins are assayed and stored at -196 C. (liquid nitrogen).The enzyme reaction measures the conversion of stearic acid (C18:0 fatty acid) to oleic acid (C18:1 fatty acid) by SCD-1. The enzyme reaction is started by adding 125 ug of HepG2 cell microsomal fraction to tubes (total reaction volume of 500 ul) containing 62 uM of stearic acid.		24	ALA3639020	cell-based format	BindingDB Database
3.	ALA888967	Binding	Inhibition of human SCD1	Homo sapiens	36	ALA1144654	single protein format	Scientific Literature
4.	ALA1071957	Binding	Inhibition of SCD1 in human 293A cells assessed as conversion of [14C]stearate to [14C]oleate after 60 mins in presence of S9 microsomal fraction	Homo sapiens	29	ALA1153242	cell-based format	Scientific Literature
5.	ALA1071959	Binding	Inhibition of human SCD1 expressed in human 293A cells assessed as conversion of [14C]stearate to [14C]oleate	Homo sapiens	23	ALA1153242	cell-based format	Scientific Literature
6.	ALA1071960	Binding	Inhibition of SCD1 in human liver S9 microsomal fraction assessed as conversion of [14C]stearate to [14C]oleate after 60 mins at 0.4 uM	Homo sapiens	1	ALA1153242	microsome format	Scientific Literature
7.	ALA1071885	Binding	Inhibition of human Scd1 expressed in HEK293A cells assessed as conversion of [14C]stearate to [14C]oleate pretreated for 30 mins measured after 4 hrs	Homo sapiens	16	ALA1153136	cell-based format	Scientific Literature
8.	ALA1071886	Binding	Inhibition of Scd1 in human HEK293A cell microsomes assessed as conversion of [14C]stearate to [14C]oleate pretreated for 10 mins measured after 60 mins	Homo sapiens	20	ALA1153136	microsome format	Scientific Literature
9.	ALA1071887	Binding	Inhibition of human Scd1 assessed as conversion of [14C]stearate to [14C]oleate at 10 uM pretreated for 30 mins measured after 4 hrs	Homo sapiens	8	ALA1153136	single protein format	Scientific Literature
10.	ALA1106892	Binding	Inhibition of SCD1 in human HepG2 cells assessed as [14C]stearic acid to [14C]oleic acid conversion pretreated 15 mins before [14C]stearic acid addition measured after 4 hrs by HPLC based scintillation assay	Homo sapiens	23	ALA1154928	cell-based format	Scientific Literature
11.	ALA1272959	Binding	Inhibition of human SCD1	Homo sapiens	19	ALA1268980	single protein format	Scientific Literature
12.	ALA1285216	Binding	Inhibition of human SCD1	Homo sapiens	2	ALA1275283	single protein format	Scientific Literature
13.	ALA1285217	Binding	Inhibition of SCD1 in HEK293A cell microsomes assessed as reduction in conversion of [14C]stearic acid to [14C]oleic acid after 30 mins	Homo sapiens	9	ALA1275283	microsome format	Scientific Literature
14.	ALA1285219	Binding	Inhibition of human SCD1 expressed in HEK293A cells assessed as reduction in conversion of [14C]stearic acid to [14C]oleic acid after 30 mins	Homo sapiens	9	ALA1275283	cell-based format	Scientific Literature
15.	ALA1776043	Binding	Inhibition of human liver microsome SCD1	Homo sapiens	5	ALA1772935	assay format	Scientific Literature
16.	ALA1776044	Binding	Inhibition of human SCD1 transfected in human 293A cells after 30 mins by whole cell assay	Homo sapiens	6	ALA1772935	cell-based format	Scientific Literature
17.	ALA1776045	Binding	Inhibition of human liver microsome SCD1 at 2 uM	Homo sapiens	1	ALA1772935	assay format	Scientific Literature
18.	ALA1839320	Binding	Inhibition of SCD1 in human HepG2 cells	Homo sapiens	19	ALA1833926	cell-based format	Scientific Literature
19.	ALA1919934	Binding	Inhibition of SCD in human HepG2 cells assessed as conversion of [1-(14)C]-stearic acid to (14)C-oleic acid	Homo sapiens	4	ALA1914316	cell-based format	Scientific Literature
20.	ALA3396817	Binding	Inhibition of SCD1 in human HepG2 cells using [14C]-stearate substrate assessed as decreased production of [14C]-oleic acid after 24 hrs	Homo sapiens	29	ALA3393027	cell-based format	Scientific Literature