| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA1024870 | B | Inhibition of HSL in Wistar rat isolated fat cells by spectrophotometric assay | Rattus norvegicus | 130 | assay format | Scientific Literature | ||
| 2. | ALA1024871 | B | Inhibition of HSL in Wistar rat isolated fat cells at 1 uM by spectrophotometric assay | Rattus norvegicus | 15 | assay format | Scientific Literature | ||
| 3. | ALA1024872 | B | Inhibition of HSL in Wistar rat isolated fat cells at 10 uM by spectrophotometric assay | Rattus norvegicus | 22 | assay format | Scientific Literature | ||
| 4. | ALA3404384 | B | Inhibition of rat hormone sensitive lipase assessed as oleic acid release from [3H]triolein by scintillation counting | Rattus norvegicus | 19 | single protein format | Scientific Literature | ||
| 5. | ALA3404385 | B | Irreversible inhibition of rat hormone sensitive lipase assessed as relative residual activity by kinetic analysis | Rattus norvegicus | 1 | single protein format | Scientific Literature | ||
| 6. | ALA3706130 | B | Inhibition Assay: To prepare the substrate, 25-50 uCi of [3H]trioleoylglycerol (in toluene), 6.8 umol of unlabeled trioleoylglycerol and 0.6 mg of phospholipids (phosphatidylcholine/phosphatidylinositol 3:1 w/v) are mixed, dried with N2 and then taken up in 2 ml of 0.1 M KPi (pH 7.0) by ultrasound treatment (Branson 250, microtip, setting 1-2, 2x1 min with an interval of 1 min). After addition of 1 ml of KPi and renewed ultrasound treatment (4x30 sec on ice with intervals of 30 sec), 1 ml of 20% BSA (in KPi) is added (final concentration of trioleoylglycerol 1.7 mM). For the reaction, 100 ul of substrate solution are pipetted into 100 ul of HSL solution (HSL prepared as above, diluted in 20 mM KPi, pH 7.0, 1 mM EDTA, 1 mM DTT, 0.02% BSA, 20 ug/ml pepstatin, 10 ug/ml leupeptin) and incubated at 37 C. for 30 min. Addition of 3.25 ml of methanol/chloroform/heptane (10:9:7) and of 1.05 ml of 0.1 M K2CO3, 0.1 M boric acid (pH 10.5) is followed by thorough mixing. | 7 | single protein format | BindingDB Database | |||
| 7. | ALA4044731 | B | Inhibition of HSL in rat adipocytes assessed as reduction in lipolysis by measuring glycerol concentration incubated for 30 mins by spectrophotometric analysis | Rattus norvegicus | 15 | cell-based format | Scientific Literature |
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