#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA988258	B	Inhibition of human recombinant AKR1C4	Homo sapiens	1	ALA1155846	single protein format	Scientific Literature
2.	ALA980736	B	Inhibition of human recombinant type 1 3-alpha-HSD expressed in Escherichia coli JM109	Homo sapiens	3	ALA1138501	assay format	Scientific Literature
3.	ALA2025088	B	Inhibition of recombinant AKR1C4 assessed as enzyme catalyzed oxidation of S-tetralol by fluorimetric assay		4	ALA2021904	single protein format	Scientific Literature
4.	ALA2038587	B	Inhibition of human recombinant GST-tagged AKR1C4 expressed in Escherichia coli using S-tetralol as substrate by fluorometry	Homo sapiens	2	ALA2034893	assay format	Scientific Literature
5.	ALA2038591	B	Inhibition of human recombinant GST-tagged AKR1C4 expressed in Escherichia coli using S-tetralol as substrate at 100 uM by fluorometry	Homo sapiens	5	ALA2034893	assay format	Scientific Literature
6.	ALA2045964	B	Inhibition of recombinant AKR1C4 assessed as NADP+ dependent oxidation of S-tetralol by fluorescence assay		9	ALA2040753	single protein format	Scientific Literature
7.	ALA2071726	B	Inhibition of recombinant AKR1C4 using S-tetralol as substrate by fluorimetry	Homo sapiens	5	ALA2069201	single protein format	Scientific Literature
8.	ALA2174985	B	Inhibition of human recombinant N-terminal His6-tagged AKR1C4 expressed in Escherichia coli BL21(DE3) cells using 8-Acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-aza-benzo[de]anthracen-10-one as substrate at 3 uM after 1 hr by fluorimetric analysis	Homo sapiens	1	ALA2169839	cell-based format	Scientific Literature
9.	ALA2174991	B	Inhibition of human recombinant N-terminal His6-tagged AKR1C4 expressed in Escherichia coli BL21(DE3) cells using 8-Acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-aza-benzo[de]anthracen-10-one as substrate after 1 hr by fluorimetric analysis	Homo sapiens	74	ALA2169839	cell-based format	Scientific Literature
10.	ALA2175100	B	Inhibition of human recombinant AKR1C4 expressed in Escherichia coli BL21(DE3) using phenanthrenequinone as substrate by spectrophotometric analysis	Homo sapiens	1	ALA2169839	assay format	Scientific Literature
11.	ALA2175688	B	Inhibition of human recombinant AKR1C4 assessed as S-tetralol oxidation at 300 uM by micro plate fluorescence reader based assay	Homo sapiens	2	ALA2169790	single protein format	Scientific Literature
12.	ALA2329811	B	Inhibition of human recombinant AKR1C4-mediated NADP+-dependent oxidation of S-(+)-1,2,3,4-tetrahydro-1-naphthol	Homo sapiens	11	ALA2321830	single protein format	Scientific Literature
13.	ALA2346174	B	Inhibition of His-tagged human AKR1C4 expressed in Escherichia coli BL21 (DE3) assessed as inhibition of reduction of non-fluorescent ketone probe to fluorescent alcohol after 1 hr by competitive fluorescence assay	Homo sapiens	14	ALA2331317	assay format	Scientific Literature
14.	ALA2346179	B	Inhibition of AKR1C4 (unknown origin)	Homo sapiens	3	ALA2331317	single protein format	Scientific Literature
15.	ALA3108468	B	Inhibition of recombinant AKR1C4 (unknown origin) after 1 hr by competitive fluorescence assay	Homo sapiens	29	ALA3102819	single protein format	Scientific Literature
16.	ALA3388495	B	Inhibition of AKR1C4 (unknown origin) assessed as dehydrogenase activity of enzyme by NADPH fluorescence assay	Homo sapiens	3	ALA3351901	single protein format	Scientific Literature
17.	ALA3541802	A	Activity of human recombinant AKR1C4 expressed in HEK293 cells assessed as (1R,2S,5S)-N-((2S,3R)-4-amino-1-cyclobutyl-3-hydroxy-4-oxobutan-2-yl)-3-((S)-2-(3-tert-butylureido)-3,3-dimethylbutanoyl)-6,6-dimethyl-3-azabicyclo[3.1.0]hexane-2-carboxamide formation per mg protein after 120 mins in presence of NADPH by LC-MS/MS/FSA method	Homo sapiens	1	ALA3526141	cell-based format	Scientific Literature
18.	ALA3887161	B	Discontinuous Radiometric Assay: Compounds may be evaluated as selective reversible inhibitors of AKR1C3 by screening them against homogeneous recombinant AKR1C1-AKR1C4 expressed in E. coli. In each case, a discontinuous radiometric assay may be used to monitor the inhibition of progesterone reduction (20-ketosteroid reduction) catalyzed by AKR1C1, the inhibition of Δ4-AD reduction (17-ketosteroid reduction) catalyzed by AKR1C3, and the inhibition of 5α-DHT reduction (3-ketosteroid reduction) catalyzed by AKR1C2 and AKR1C4 (by measuring the formation of 20α-hydroxyprogesterone, testosterone or 3α-androstanediol by radiochromatography). Secondary screens of the compounds of interest include: (a) a full-screen against all nine human recombinant AKR enzymes to ensure there are no-intended off-target effects (in this context AKR1B10 (retinal reductase; SEQ ID NO:5) has been shown to be potently inhibited by N-phenylanthranilates) (Endo et al., 2010, Biol. Pharm. Bull. 33:886-90); (b) a screen against COX-1 and COX-2 to reaffirm that compounds do not act as NSAIDs; and (c) an expanded screen against other nuclear receptors (especially other steroid hormone receptors).		10	ALA3886196	assay format	BindingDB Database
19.	ALA3888494	B	Inhibition Assay: Inhibitors were initially screened for an ability to block the NADP+ dependent oxidation of the artificial substrate S-tetralol catalyzed by AKR1C3. S-tetralol was used since it is also a substrate of the highly related AKR1C1 and AKR1C2 enzymes. Inhibition of AKR1C1 and AKR1C2 is undesirable in the context of prostate cancer since they are involved in the elimination of DHT (see FIG. 1B; see also Rizner et al, 2003; Steckelbroeck et al. 2004). All assays were performed at Km so that IC50 values across enzyme forms were comparable.An exemplary screening strategy was against homogeneous recombinant AKR1C3 and its highly related enzyme AKR1C2 to generate full dose-response curves and IC50 values.		11	ALA3886729	single protein format	BindingDB Database
20.	ALA4020435	B	Inhibition of recombinant human AKR1C4 using S-tetralol as substrate	Homo sapiens	2	ALA4017540	single protein format	Scientific Literature