#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA931722	B	Inhibition of Plasmodium falciparum PNP	Plasmodium falciparum	6		single protein format	Scientific Literature
2.	ALA982802	B	Inhibition of Plasmodium falciparum purine nucleoside phosphorylase assessed as slow onset inhibition constant by xanthine-oxidase coupled assay	Plasmodium falciparum	4		single protein format	Scientific Literature
3.	ALA982804	B	Inhibition of Plasmodium falciparum purine nucleoside phosphorylase by xanthine-oxidase coupled assay	Plasmodium falciparum	3		single protein format	Scientific Literature
4.	ALA1287512	B	Inhibition of Plasmodium falciparum PNP expressed in Escherichia coli BL21(DE3) cells by spectrophotometry	Plasmodium falciparum	12		cell-based format	Scientific Literature
5.	ALA3606855	B	Inhibition of recombinant Plasmodium falciparum PNP using inosine as substrate by xanthine oxidase coupling enzyme assay	Plasmodium falciparum	2		single protein format	Scientific Literature
6.	ALA3606856	B	Inhibition of recombinant Plasmodium falciparum PNP using inosine as substrate assessed as inhibition constant for slow onset inhibition of enzyme-inhibitor complex by xanthine oxidase coupling enzyme assay	Plasmodium falciparum	2		single protein format	Scientific Literature
7.	ALA3707659	B	Inhibition Assay: The inhibitor dissociation constants reported in Table 1 below are for phosphorolysis of inosine by PNP and were based on reaction rates measurements with different inhibitor concentrations. Reactions were started by addition of 0.05 ug of human or Plasmodium falciparum purine nucleoside phosphaorylase (HsPNP and PfPNP, respectively; final concentration 1.4 nM) to 1 mM inosine in 50 mM KPO4, pH=7.5 buffer with xanthine oxidase added to final concentration 60 mU/mL at 25 C. In the coupled assay, hypoxanthine formed by phosphorolysis of inosine was oxidized to uric acid and followed spectrophotometrically at 293 nm (extinction coefficient for uric acid epsilon(293)=12.9 mM-1). The dissociation constant for slow-onset tight-binding inhibitors was determined from reaction rates after slow onset inhibition had occurred according to the equation u =(kcat x S)/(Km(1+l/Kd)+S), where u  is the steady state reaction rate after the slow-onset inhibition period has reached equilibrium.	Plasmodium falciparum	31		single protein format	BindingDB Database
8.	ALA4377121	B	Binding affinity to Plasmodium falciparum His-tagged PNP assessed as catalytic rate by measuring uric acid formation	Plasmodium falciparum	1		single protein format	Scientific Literature
9.	ALA4377138	B	Inhibition of Plasmodium falciparum His-tagged PNP assessed as inhibitor constant for enzyme-inhibitor complex formation	Plasmodium falciparum	4		single protein format	Scientific Literature
10.	ALA4377139	B	Inhibition of Plasmodium falciparum His-tagged PNP assessed as inhibitor constant for enzyme-inhibitor-substrate complex formation	Plasmodium falciparum	4		single protein format	Scientific Literature
11.	ALA4377148	B	Binding affinity to Plasmodium falciparum His6-tagged PNP assessed as reduction in uric acid formation using inosine as substrate	Plasmodium falciparum	2		single protein format	Scientific Literature