| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA979097 | B | Inhibition of human recombinant DNA ligase 1 at 100 uM by fluorescence energy transfer-based DNA joining assay relative to control | Homo sapiens | 10 | ALA1155879 | single protein format | Scientific Literature | |
| 2. | ALA979101 | B | Inhibition of ethidium bromide binding to human recombinant DNA ligase 1 at 100 uM | Homo sapiens | 4 | ALA1155879 | single protein format | Scientific Literature | |
| 3. | ALA979102 | B | Inhibition of human recombinant DNA ligase 1 in presence of 0.1% Triton X-100 by fluorescence energy transfer-based DNA joining assay relative to control | Homo sapiens | 7 | ALA1155879 | single protein format | Scientific Literature | |
| 4. | ALA979103 | B | Inhibition of human recombinant DNA ligase 1 by radioactive gel-based ligation assay | Homo sapiens | 10 | ALA1155879 | single protein format | Scientific Literature | |
| 5. | ALA979104 | B | Inhibition of human recombinant DNA ligase 1 using nicked DNA substrate by kinetic assay | Homo sapiens | 1 | ALA1155879 | single protein format | Scientific Literature | |
| 6. | ALA1810900 | B | Inhibition of human DNA Ligase I by FRET-based assay | Homo sapiens | 1 | ALA1806435 | single protein format | Scientific Literature | |
| 7. | ALA2034653 | B | Inhibition of human ATP-dependent DNA ligase 1 | Homo sapiens | 25 | ALA2029313 | single protein format | Scientific Literature | |
| 8. | ALA2166645 | B | Inhibition of human DNA Ligase 1 | Homo sapiens | 29 | ALA2163244 | single protein format | Scientific Literature | |
| 9. | ALA2166649 | B | Inhibition of human DNA Ligase 1 at 25 uM | Homo sapiens | 1 | ALA2163244 | single protein format | Scientific Literature | |
| 10. | ALA3224682 | B | Inhibition of nick joining activity of human DNA ligase 1 using [gamma32P]ATP-labeled nicked 40 bp DNA as substrate | Homo sapiens | 4 | ALA3217431 | single protein format | Scientific Literature | |
| 11. | ALA3224635 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21 (DE3) using 22-mer and 18-mer DNA complementary strand as substrate after 1 hr by 15% polyacrylamide gel electrophoresis | Homo sapiens | 7 | ALA3217442 | assay format | Scientific Literature | |
| 12. | ALA4142317 | B | Inhibition of human Lig1 at 10 uM using double strand nicked DNA as substrate after 30 mins in presence of ATP by acrylamide-based fluorescence-assay | Homo sapiens | 13 | ALA4138186 | single protein format | Scientific Literature | |
| 13. | ALA4142332 | B | Competitive inhibition of human Lig1 using non-ligatable double stranded nicked DNA as substrate after 2 hrs in presence of ATP by EMSA | Homo sapiens | 1 | ALA4138186 | single protein format | Scientific Literature | |
| 14. | ALA4190757 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as DNA ligation at 100 uM using fluorescently labelled ds nicked DNA as substrate after 30 mins by urea gel based imaging method relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 15. | ALA4190758 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as reduction in fluorescently labelled ds nicked DNA substrate ligation at 100 uM after 30 mins by urea gel based imaging method relative to control | Homo sapiens | 19 | ALA4190183 | cell-based format | Scientific Literature | |
| 16. | ALA4190759 | B | Binding affinity to human full length N-terminal His-tagged DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells at 10 uM by fluorescence spectroscopic analysis | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 17. | ALA4190766 | B | Inhibition of human DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells assessed as reduction in nick sealing activity using fluorescently labelled ds nicked DNA as substrate after 30 mins by urea gel based imaging method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 18. | ALA4190767 | B | Binding affinity to human full length N-terminal His-tagged DNA ligase 1 expressed in Escherichia coli BL21(DE3)RP cells at 1.25 to 5 uM by CD spectroscopic method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 19. | ALA4190768 | B | Binding affinity to human N-terminal His-tagged DNA ligase 1 DBD expressed in Escherichia coli BL21(DE3)RP cells at 1.25 to 5 uM by CD spectroscopic method | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature | |
| 20. | ALA4190769 | B | Binding affinity to human N-terminal His-tagged DNA ligase 1 DBD expressed in Escherichia coli BL21(DE3)RP cells at 10 uM by fluorescence spectroscopic analysis | Homo sapiens | 1 | ALA4190183 | cell-based format | Scientific Literature |
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