# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA2149690 | B | Increase of calf intestine alkaline phosphatase activity assessed as production of p-nitrophenol using p-nitrophenylphosphate as substrate at 50 mM after 3 mins by spectrophotometer | Bos taurus | 1 | single protein format | Scientific Literature | ||
2. | ALA996833 | B | Inhibition of calf recombinant intestinal alkaline phosphatase at 10 uM | Bos taurus | 4 | single protein format | Scientific Literature | ||
3. | ALA1068634 | B | Inhibition of bovine intestinal alkaline phosphatase assessed as residual enzyme activity at 0.1 mM in presence of 1% DMSO at pH 10.4 at 37 degC relative to untreated control | Bos taurus | 127 | single protein format | Scientific Literature | ||
4. | ALA1177099 | B | Inhibition of bovine intestinal alkaline phosphatase | Bos taurus | 1 | single protein format | Scientific Literature | ||
5. | ALA2379311 | B | Inhibition of calf intestinal alkaline phosphatase using p-NPP as substrate incubated for 10 mins prior to substrate addition measured after 30 mins by spectrophotometry | Bos taurus | 1 | single protein format | Scientific Literature | ||
6. | ALA2411273 | B | Competitive inhibition of bovine intestinal alkaline phosphatase using p-NPP as substrate by Double-reciprocal plot analysis | Bos taurus | 1 | single protein format | Scientific Literature | ||
7. | ALA2411276 | B | Inhibition of bovine intestinal alkaline phosphatase using p-NPP as substrate treated 10 mins before substrate addition measured after 30 mins by spectrophotometric assay | Bos taurus | 21 | single protein format | Scientific Literature | ||
8. | ALA3239865 | B | Inhibition of calf intestinal alkaline phosphatase using p-nitrophenyl phosphate as substrate preincubated for 10 mins before substrate addition after 30 mins | Bos taurus | 22 | single protein format | Scientific Literature | ||
9. | ALA3258713 | B | Inhibition of calf intestine alkaline phosphatase using p-nitrophenyl phosphate as substrate at 8.7 x 10'-5 M up to 35 mins by spectrophotometry | Bos taurus | 3 | single protein format | Scientific Literature | ||
10. | ALA3404592 | B | Inhibition of calf intestinal alkaline phosphatase using CDP-star chemiluminescent substrate assessed as change in luminescence by spectrophotometric method | Bos taurus | 19 | single protein format | Scientific Literature | ||
11. | ALA3590931 | B | Activity of calf intestinal phosphatase assessed as compound turn over rates by inorganic phosphate release and malachite green dye based inorganic phosphate detection based assay | Bos taurus | 2 | single protein format | Scientific Literature | ||
12. | ALA3776499 | B | Inhibition of bovine intestinal alkaline phosphatase using para-nitrophenylphosphate as substrate preincubated for 10 mins at pH 7.8 followed by substrate addition | Bos taurus | 4 | single protein format | Scientific Literature | ||
13. | ALA3776735 | B | Inhibition of bovine intestinal alkaline phosphatase using para-nitrophenylphosphate as substrate preincubated for 10 mins at pH 10.4 followed by substrate addition | Bos taurus | 4 | single protein format | Scientific Literature | ||
14. | ALA3804258 | B | Inhibition of calf intestinal alkaline phosphatase preincubated for 3 to 5 mins followed by CDP-star substrate addition measured after 15 mins by luminescence assay | Bos taurus | 19 | single protein format | Scientific Literature | ||
15. | ALA3804261 | B | Competitive inhibition of calf intestinal alkaline phosphatase at 0.5 to 2 uM preincubated for 3 to 5 mins followed by CDP-star substrate addition measured after 15 mins by double-reciprocal plot analysis | Bos taurus | 1 | single protein format | Scientific Literature | ||
16. | ALA4003043 | B | Inhibition of calf intestinal alkaline phosphatase using CDP-star as substrate preincubated for 5 to 7 mins followed by substrate addition measured after 15 mins by spectrophotometric method | Bos taurus | 21 | single protein format | Scientific Literature | ||
17. | ALA4003045 | B | Inhibition of calf intestinal alkaline phosphatase at 0.2 mM using CDP-star as substrate preincubated for 5 to 7 mins followed by substrate addition measured after 15 mins by spectrophotometric method | Bos taurus | 3 | single protein format | Scientific Literature | ||
18. | ALA4003046 | B | Potency index, ratio of L-phenylalanine IC50 to test compound IC50 for calf intestinal alkaline phosphatase | Bos taurus | 2 | single protein format | Scientific Literature | ||
19. | ALA4054304 | B | Inhibition of calf intestinal alkaline phosphate at 0.1 to 100 uM using UDP as substrate after 20 mins by malachite green dye-based assay | Bos taurus | 2 | single protein format | Scientific Literature | ||
20. | ALA4125113 | B | Mixed-type inhibition of calf intestinal alkaline phosphatase assessed as substrate Vmax at 0.015 uM using varying levels of p-NPP as substrate measured every minute for 10 mins by Lineweaver-Burk plot analysis (Rvb = 1.2 10'-5deltaA/s) | Bos taurus | 1 | single protein format | Scientific Literature |