# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1032227 | B | Inhibition of CDC2L1 at 3 uM | 1 | ALA1142492 | single protein format | Scientific Literature | ||
2. | ALA1060184 | B | Binding constant for CDC2L1 kinase domain | Homo sapiens | 38 | ALA1150977 | single protein format | Scientific Literature | |
3. | ALA1052881 | B | Inhibition of CDC2L1 assessed as enzyme activity at 1 uM relative to untreated control | 1 | ALA1155237 | single protein format | Scientific Literature | ||
4. | ALA1168817 | B | Inhibition of CDC2L1 at 1 uM | 6 | ALA1165965 | single protein format | Scientific Literature | ||
5. | ALA1174100 | B | Inhibition of CDC2L1 at 10 uM | 2 | ALA1177794 | single protein format | Scientific Literature | ||
6. | ALA1244755 | B | Binding affinity to CDC2L1 | 7 | ALA1240346 | single protein format | Scientific Literature | ||
7. | ALA1827288 | B | Activity of CDC2L1 kinase at 1 uM | 1 | ALA1821651 | single protein format | Scientific Literature | ||
8. | ALA1908405 | B | Binding constant for CDC2L1 kinase domain | 72 | ALA1908390 | single protein format | Scientific Literature | ||
9. | ALA2209340 | B | Inhibition of CDC2L1 assessed as residual activity at 10 uM relative to control | 1 | ALA2203081 | single protein format | Scientific Literature | ||
10. | ALA2422449 | B | Inhibition of CDK11 in human PC3 cell lysates at 10 uM using desthiobiotin-tagged ATP probe AX9989 followed by trypsinization by LC/MS analysis | Homo sapiens | 12 | ALA2417377 | single protein format | Scientific Literature | |
11. | ALA2428176 | B | Competitive binding affinity to CDK11 (unknown origin) at 10 uM in presence of ATP relative to control | Homo sapiens | 3 | ALA2424622 | single protein format | Scientific Literature | |
12. | ALA2428178 | B | Competitive binding affinity to CDC2L1 (unknown origin) at 10 uM in presence of ATP relative to control | Homo sapiens | 4 | ALA2424622 | single protein format | Scientific Literature | |
13. | ALA2429327 | B | Binding affinity to CDC2L1 (unknown origin) | Homo sapiens | 1 | ALA2424622 | single protein format | Scientific Literature | |
14. | ALA2439141 | B | Inhibition of human wild type CDK11 at 1 uM by qPCR analysis relative to control | Homo sapiens | 1 | ALA2434948 | single protein format | Scientific Literature | |
15. | ALA3112119 | B | Inhibition of CDC2L1 (unknown origin) assessed as residual activity at 10 uM after 1 hr by qPCR analysis relative to control | Homo sapiens | 1 | ALA3108735 | single protein format | Scientific Literature | |
16. | ALA3630639 | B | Inhibition of CDK11 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control | Homo sapiens | 1 | ALA3627674 | single protein format | Scientific Literature | |
17. | ALA3707511 | B | Kinase Screen Assay: KINOMEscan (Ambit Biosciences, San Diego, Calif.), a high-throughput method for screening small molecular agents against a large panel of human kinases, was utilized for compound 2. The technology is a competition binding assay that profiled the selectivity of compound 2 against 350 kinases, each fused to a proprietary tag. The quantity of each kinase bound to an immobilized, active site-directed ligand was measured in the presence and absence of compound 2. | Homo sapiens | 1 | ALA3639044 | single protein format | BindingDB Database | |
18. | ALA3773959 | B | Inhibition of human recombinant CDK11 assessed as residual activity at 10 uM after 1 hr by qPCR analysis relative to control | Homo sapiens | 1 | ALA3769377 | single protein format | Scientific Literature | |
19. | ALA3812231 | B | Inhibition of human CDK11 (1 to 360 amino acid residues) assessed as enzyme activity at 10 uM by KINOMEScan assay expressed in bacterial system assessed as enzyme activity at 10 uM by KINOMEScan assay | Homo sapiens | 1 | ALA3808372 | assay format | Scientific Literature | |
20. | ALA3887935 | B | Competitive Binding Assay (KINOMEscan): For the competitive binding assay, test compounds were screened at 10 uM against 70 diverse kinase targets for competitive binding using the KINOMEscan technology (Ambit Biosciences) (Karaman, et al. (2008) Nature biotechnology 26:127-132). 11-point Kd curves were determined for hits identified during the initial screen. | Homo sapiens | 1 | ALA3886510 | single protein format | BindingDB Database |