| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2049708 | B | Inhibition of recombinant fluorescein-tagged E1 assessed as inhibition of ubiquitin-E1 adduct formation incubated for 15 mins prior to ATP addition measured after 5 mins by SDS PAGE analysis | 1 | single protein format | Scientific Literature | |||
| 2. | ALA2049709 | B | Inhibition of ubiquitin-activating enzyme E1 | 1 | single protein format | Scientific Literature | |||
| 3. | ALA2049710 | B | Inhibition of GST-tagged ubiquitin binding to FLAG-tagged E1 at 50 uM after 20 mins by immunoprecipitation analysis | 3 | single protein format | Scientific Literature | |||
| 4. | ALA2050671 | B | Inhibition of FLAG-tagged ubiquitin-activating enzyme E1 assessed as inhibition of GST-ubiquitin/FLAG-E1 intermediate formation at 100 uM by Western blot analysis | 1 | single protein format | Scientific Literature | |||
| 5. | ALA2050672 | B | Inhibition of FLAG-tagged ubiquitin-activating enzyme E1 assessed as inhibition of GST-ubiquitin/FLAG-E1 intermediate formation by Western blot analysis | 2 | single protein format | Scientific Literature | |||
| 6. | ALA2065193 | B | Binding affinity to UBA1 in human Jurkat cells at 150 uM followed by UV irradiation for 30 mins with Wood's glass filtered medium pressure mercury arc light prior to cell lysis measured after denaturing wash by LC-MS/MS based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA2065194 | B | Binding affinity to UBA1 in human Jurkat cells at 150 uM followed by UV irradiation for 30 mins with Wood's glass filtered medium pressure mercury arc light after cell lysis measured after denaturing wash by SDS-PAGE based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA2065202 | B | Binding affinity to UBA1 in human Jurkat cells at 150 uM followed by UV irradiation for 30 mins with Wood's glass filtered medium pressure mercury arc light prior to cell lysis measured after denaturing wash by SDS-PAGE based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA1014022 | B | Inhibition of human recombinant E1-ubiquitin complex formation | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 10. | ALA3048495 | B | Inhibition of ubiquitin displacement on His6-tagged E2 enzyme from His6-tagged UBA1 (unknown origin) at 10 uM after 1.5 hr by SDS-PAGE analysis | Homo sapiens | 18 | single protein format | Scientific Literature | ||
| 11. | ALA4118695 | B | Binding affinity to UBA1 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4118871 | B | Binding affinity to UBA1 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4132946 | B | Inhibition of ubiquitin/ubiquitin-activating enzyme E1 (unknown origin) | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 14. | ALA4143722 | B | Inhibition of UAE1 (unknown origin) assessed as decrease in UAE1-mediated Ubc10-ubiquitin conjugation levels up to 10 uM preincubated for 10 mins followed by ATP and Mg2+ addition measured after 60 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4143751 | B | Inhibition of UAE1-mediated Ubc10-ubiquitin conjugation in human Caco2 cells up to 10 uM preincubated for 10 mins followed by ATP and Mg2+ addition measured after 60 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4231157 | B | Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation up to 1000 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 17. | ALA4231158 | B | Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 18. | ALA4231159 | B | Inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 3 to 300 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 30 mins by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA4231162 | B | Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 20. | ALA4231163 | B | Irreversible inhibition of recombinant human E1 expressed in Escherichia coli BL21(DE3) assessed as reduction in biotin-labelled ubiquitin-E1 conjugate formation at 10 uM pretreated for 60 mins followed by 2.5 uM biotin-labelled ubiquitin addition for 30 mins and subsequent 5 uM biotin-labelled ubiquitin addition measured after 30 mins by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature |
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