#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA995460	B	Displacement of [3H]3-chloro-4,7-difluoro-N-(4-methoxy-3-(pyridin-4-yl)benzyl)-N-(4-(methylamino)cyclohexyl)benzo[b]thiophene-2-carboxamide from human Smoothened receptor expressed in CHO-K1 cells by membrane filter binding assay	Homo sapiens	35	ALA1136988	cell-based format	Scientific Literature
2.	ALA1029071	B	Antagonist activity at human Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	Homo sapiens	21	ALA1149081	cell-based format	Scientific Literature
3.	ALA1031015	B	Inhibition of human recombinant SMO expressed in mouse C3H10T1/2 cells assessed as inhibition of association of BODIPY-cyclopamine	Homo sapiens	1	ALA1158454	cell-based format	Scientific Literature
4.	ALA1060787	B	Inhibition of BODIPY-cyclopamine to Smo in human HEK293 Flag-Smo cells in presence of 2% FCS	Homo sapiens	35	ALA1153047	cell-based format	Scientific Literature
5.	ALA1120457	B	Inhibition of human SMO expressed in HEPM cells	Homo sapiens	35	ALA1158625	cell-based format	Scientific Literature
6.	ALA1212595	F	Antagonist activity at human Smo receptor in HEPM cells assessed as inhibition of Gli expression after 24 hrs by quantigene assay	Homo sapiens	27	ALA1208732	cell-based format	Scientific Literature
7.	ALA1211948	B	Inhibition of Smoothened transfected in HEK293 cells by transient transfection cell-based assay		13	ALA1208761	cell-based format	Scientific Literature
8.	ALA1211949	B	Inhibition of Smoothened transfected in HEK293 cells by binding assay		13	ALA1208761	cell-based format	Scientific Literature
9.	ALA1220916	B	Displacement of BODIPY-cyclopamine from Smo expressed in HEK293T cells after 30 mins		1	ALA1212710	cell-based format	Scientific Literature
10.	ALA1226134	B	Inhibition of BODIPY-cyclopamine binding to Smo expressed in HEK293T cells after 1 hr by fluorescence microscopy		1	ALA1221175	cell-based format	Scientific Literature
11.	ALA1226135	B	Inhibition of BODIPY-cyclopamine binding to Smo N-terminal cysteine domain expressed in HEK293T cells at 5 uM after 4 hrs by fluorescence microscopy		1	ALA1221175	cell-based format	Scientific Literature
12.	ALA1226136	B	Inhibition of BODIPY-cyclopamine binding to Smo C-terminal cytoplasmic domain expressed in HEK293T cells at 5 uM after 4 hrs by fluorescence microscopy		1	ALA1221175	cell-based format	Scientific Literature
13.	ALA1810042	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibition of Hh pathway		57	ALA1806461	cell-based format	Scientific Literature
14.	ALA1810043	B	Displacement of [125I]-PA cyclopamine from smoothened expressed in african green monkey COS-1 cells after 10 mins by autoradiography		57	ALA1806461	cell-based format	Scientific Literature
15.	ALA1810044	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibition of Hh pathway at 2 uM		1	ALA1806461	cell-based format	Scientific Literature
16.	ALA1816318	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells assessed as inhibition of purmorphamine- induced Gli-dependent luciferase gene expression		20	ALA1811786	cell-based format	Scientific Literature
17.	ALA1816319	B	Displacement of BODIPY-cyclopamine from human Smo expressed in HEK293 cells in presence of 2% fetal calf serum	Homo sapiens	20	ALA1811786	cell-based format	Scientific Literature
18.	ALA1816320	B	Displacement of BODIPY-cyclopamine from human Smo expressed in HEK293 cells in presence of 20% normal human serum	Homo sapiens	16	ALA1811786	cell-based format	Scientific Literature
19.	ALA1816322	B	Ratio of IC50 for human Smo expressed in HEK293 cells in presence of 20% normal human serum to IC50 for human Smo expressed in HEK293 cells in presence of 2% fetal calf serum	Homo sapiens	1	ALA1811786	cell-based format	Scientific Literature
20.	ALA1827061	B	Displacement of BODIPY-cyclopamine from Smo expressed in COS-1 cells after 4 to 6 hrs by Flow Cytometry analysis in presence of 2% fetal calf serum		38	ALA1821583	cell-based format	Scientific Literature