#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1810042	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibition of Hh pathway		57		cell-based format	Scientific Literature
2.	ALA1810043	B	Displacement of [125I]-PA cyclopamine from smoothened expressed in african green monkey COS-1 cells after 10 mins by autoradiography		57		cell-based format	Scientific Literature
3.	ALA1810044	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells co-expressing Gli-dependent reporter gene assessed as inhibition of Hh pathway at 2 uM		1		cell-based format	Scientific Literature
4.	ALA1816318	F	Antagonist activity at smoothened expressed in mouse Shh Light2 cells assessed as inhibition of purmorphamine- induced Gli-dependent luciferase gene expression		20		cell-based format	Scientific Literature
5.	ALA1816319	B	Displacement of BODIPY-cyclopamine from human Smo expressed in HEK293 cells in presence of 2% fetal calf serum	Homo sapiens	20		cell-based format	Scientific Literature
6.	ALA1816320	B	Displacement of BODIPY-cyclopamine from human Smo expressed in HEK293 cells in presence of 20% normal human serum	Homo sapiens	16		cell-based format	Scientific Literature
7.	ALA1816322	B	Ratio of IC50 for human Smo expressed in HEK293 cells in presence of 20% normal human serum to IC50 for human Smo expressed in HEK293 cells in presence of 2% fetal calf serum	Homo sapiens	1		cell-based format	Scientific Literature
8.	ALA2032860	F	Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells at 0.3 uM by luciferase reporter gene assay	Homo sapiens	32		cell-based format	Scientific Literature
9.	ALA2032861	F	Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells at 3 uM by luciferase reporter gene assay	Homo sapiens	62		cell-based format	Scientific Literature
10.	ALA2033031	F	Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay	Homo sapiens	68		cell-based format	Scientific Literature
11.	ALA2033032	F	Antagonist activity at Smo receptor		3		assay format	Scientific Literature
12.	ALA2033036	B	Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy	Homo sapiens	8		cell-based format	Scientific Literature
13.	ALA2061994	B	Inhibition of Smo expressed in mouse NIH/3T3 cells after 20 hrs by Gli reporter gene assay	Homo sapiens	25		cell-based format	Scientific Literature
14.	ALA2061995	B	Inhibition of Smo expressed in mouse NIH/3T3 cells after 20 hrs by Renilla luciferase assay	Homo sapiens	1		cell-based format	Scientific Literature
15.	ALA2060771	B	Inhibition of Smo expressed in mouse C3H10T1/2 cells assessed as reduction in alkaline phosphatase level after 72 hrs by spectrophotometric analysis	Homo sapiens	20		cell-based format	Scientific Literature
16.	ALA2066208	B	Inhibition of SMO expressed in mouse C3H10T1/2 cells assessed as down-regulation of Gli1 mRNA expression at 5 uM after 24 hrs by RT-PCR analysis relative to oxysterol	Homo sapiens	14		cell-based format	Scientific Literature
17.	ALA995460	B	Displacement of [3H]3-chloro-4,7-difluoro-N-(4-methoxy-3-(pyridin-4-yl)benzyl)-N-(4-(methylamino)cyclohexyl)benzo[b]thiophene-2-carboxamide from human Smoothened receptor expressed in CHO-K1 cells by membrane filter binding assay	Homo sapiens	35		cell-based format	Scientific Literature
18.	ALA1029071	B	Antagonist activity at human Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	Homo sapiens	21		cell-based format	Scientific Literature
19.	ALA1031015	B	Inhibition of human recombinant SMO expressed in mouse C3H10T1/2 cells assessed as inhibition of association of BODIPY-cyclopamine	Homo sapiens	1		cell-based format	Scientific Literature
20.	ALA1060787	B	Inhibition of BODIPY-cyclopamine to Smo in human HEK293 Flag-Smo cells in presence of 2% FCS	Homo sapiens	35		cell-based format	Scientific Literature