| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2090685 | B | Inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using S-methyl-L-thiocitrulline as substrate at 100 uM by CPM-based fluorescence spectrophotometry | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 2. | ALA2090686 | B | Inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using Nomega,Nomega-dimethyl-L-arginine as substrate at 400 uM after 1 to 2 hrs by colorimetric ureido derivitization assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 3. | ALA2090687 | B | Inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using Nomega,Nomega-dimethyl-L-arginine as substrate at 400 uM by colorimetric ureido derivitization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 4. | ALA2090688 | B | Inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using Nomega,Nomega-dimethyl-L-arginine as substrate at 400 uM incubated for 10 to 20 mins prior to substrate addition measured after 1 to 2 hrs by colorimetric ureido derivitization assay in presence of 2 mM reduced glutathione | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 5. | ALA2090689 | B | Reversible inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using 1 mM Nomega,Nomega-dimethyl-L-arginine as substrate incubated up to 120 mins prior to substrate addition measured after 120 mins by colorimetric ureido derivitization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 6. | ALA2090690 | B | Inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using Nomega,Nomega-dimethyl-L-arginine as substrate after 80 mins by colorimetric ureido derivitization assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 7. | ALA2090691 | B | Competitive inhibition of human recombinant His6-tagged DDAH1 expressed in Escherichia coli BL21(DE3) using Nomega,Nomega-dimethyl-L-arginine as substrate after 80 mins by double-reciprocal plot analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 8. | ALA939399 | B | Inhibition of human recombinant DDAH1 expressed in Escherichia coli BL21 cells | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 9. | ALA981884 | B | Activity at human recombinant wild type DDAH1 | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 10. | ALA981885 | B | Activity at human DDAH1 C275A mutant | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 11. | ALA981886 | B | Activity at human DDAH1 H173A mutant | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA981890 | B | Ratio of Kcat to Km for human recombinant wild type DDAH1 | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 13. | ALA981891 | B | Ratio of Kcat to Km for human DDAH1 C275A mutant | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 14. | ALA981895 | B | Inhibition of human recombinant DDAH1 after 20 mins | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA981896 | B | Inhibition of human recombinant DDAH1 | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA981898 | B | Inhibition of human recombinant DDAH1 assessed as inactivation rate at 0.2 mM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA981899 | B | Inhibition of human recombinant DDAH1 assessed as inactivation rate at 0.4 mM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA981900 | B | Inhibition of human recombinant DDAH1 assessed as inactivation rate at 0.6 mM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA981901 | B | Inhibition of human recombinant DDAH1 assessed as inactivation rate at 0.8 mM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA981902 | B | Inhibition of human recombinant DDAH1 assessed as inactivation rate at 1.5 mM | Homo sapiens | 1 | single protein format | Scientific Literature |
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