| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2045142 | B | Inhibition of Smo in mouse C3H10T1/2 cells using human recombinant SHH assessed as effect on SMO/SHH transient transcriptional activation after 20 hrs by Gli-luciferase reporter assay | Mus musculus | 12 | cell-based format | Scientific Literature | ||
| 2. | ALA2045182 | B | Potency ratio, ratio of IC50 for compound to IC50 for compound 1 in mouse C3H10T1/2 cells by SMO/SHH transient transcriptional activation based Gli-luciferase reporter assay | Mus musculus | 10 | cell-based format | Scientific Literature | ||
| 3. | ALA1825811 | F | Antagonist activity at mouse SMO expressed in NIH/3T3 cells transfected with Gli-dependent luciferase-reporter after 15 hrs by luciferase reporter gene assay using luminometer | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA2161253 | F | Antagonist activity at Smo in mouse TM3 cells expressing Hh12 assessed as inhibition of 1 nM Ag1.5-induced Gli expression incubated for 30 mins prior to Ag1.5-induction measured after 48 hrs by luciferase reporter gene assay | Mus musculus | 14 | cell-based format | Scientific Literature | ||
| 5. | ALA2161254 | F | Antagonist activity at Smo in mouse TM3 cells expressing Hh12 assessed as inhibition of 25 nM Ag1.5-induced Gli expression incubated for 30 mins prior to Ag1.5-induction measured after 48 hrs by luciferase reporter gene assay | Mus musculus | 11 | cell-based format | Scientific Literature | ||
| 6. | ALA2161258 | B | Antagonist activity at mouse Smo expressed in CHO cells assessed as inhibition of BODIPY-cyclopamine binding after 4 hrs by fluorescence assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA2162708 | F | Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase reporter gene method | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 8. | ALA2155377 | B | Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced cell differentiation after 48 hrs by alkaline phosphatase reporter assay | Mus musculus | 28 | cell-based format | Scientific Literature | ||
| 9. | ALA2155378 | B | Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced cell differentiation after 48 hrs by alkaline phosphatase reporter assay relative to cyclopamine | Mus musculus | 23 | cell-based format | Scientific Literature | ||
| 10. | ALA2155379 | B | Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA2155380 | B | Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis relative to cyclopamine | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA2155381 | B | Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis in presence of SAG | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 13. | ALA2155382 | B | Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli1 transcriptional activity | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 14. | ALA2155383 | B | Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 15. | ALA2155384 | B | Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay in presence of SAG | Mus musculus | 3 | cell-based format | Scientific Literature | ||
| 16. | ALA2153865 | B | Competitive inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 55.5 to 4500 nM by qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA2153866 | B | Competitive inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 4.1 to 1000 nM by qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA2153868 | B | Allosteric inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 103 to 25000 nM by qPCr analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 19. | ALA2153870 | B | Allosteric inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of purmorphamine-induced Gli1 transcriptional activity at 4.1 to 1000 nM by qPCr analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA2153871 | B | Competitive inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of purmorphamine-induced Gli1 transcriptional activity at 103 to 25000 nM by qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature |
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