#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA995457	B	Inhibition of Smoothened receptor in mouse TM3 cells transfected with pTA-8xGli-Luc reporter gene assessed as inhibition of 25 nM 3-chloro-4,7-difluoro-N-(4-methoxy-3-(pyridin-4-yl)benzyl)-N-(4-(methylamino)cyclohexyl)benzo[b]thiophene-2-carboxamide-induced Hedgehog pathway activation by Gli shift assay	Mus musculus	35	ALA1136988	cell-based format	Scientific Literature
2.	ALA995458	B	Inhibition of Smoothened receptor in mouse TM3 cells transfected with pTA-8xGli-Luc reporter gene assessed as inhibition of 1 nM 3-chloro-4,7-difluoro-N-(4-methoxy-3-(pyridin-4-yl)benzyl)-N-(4-(methylamino)cyclohexyl)benzo[b]thiophene-2-carboxamide-induced Hedgehog pathway activation by Gli shift assay	Mus musculus	35	ALA1136988	cell-based format	Scientific Literature
3.	ALA995459	B	Displacement of [3H]3-chloro-4,7-difluoro-N-(4-methoxy-3-(pyridin-4-yl)benzyl)-N-(4-(methylamino)cyclohexyl)benzo[b]thiophene-2-carboxamide from mouse Smoothened receptor expressed in CHO-K1 cells by membrane filter binding assay	Mus musculus	35	ALA1136988	cell-based format	Scientific Literature
4.	ALA1029070	B	Antagonist activity at mouse Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	Mus musculus	28	ALA1149081	cell-based format	Scientific Literature
5.	ALA1120458	B	Inhibition of mouse SMO expressed in NIH-3T3 cells	Mus musculus	1	ALA1158625	cell-based format	Scientific Literature
6.	ALA1212594	B	Antagonist activity at mouse cloned Smo receptor expressed in NIH-3T3 cells co expressing Gli1 binding site after 15 hrs by luciferase reporter gene assay	Mus musculus	3	ALA1208732	cell-based format	Scientific Literature
7.	ALA1825811	F	Antagonist activity at mouse SMO expressed in NIH/3T3 cells transfected with Gli-dependent luciferase-reporter after 15 hrs by luciferase reporter gene assay using luminometer	Mus musculus	2	ALA1821579	cell-based format	Scientific Literature
8.	ALA2045142	B	Inhibition of Smo in mouse C3H10T1/2 cells using human recombinant SHH assessed as effect on SMO/SHH transient transcriptional activation after 20 hrs by Gli-luciferase reporter assay	Mus musculus	12	ALA2040751	cell-based format	Scientific Literature
9.	ALA2045182	B	Potency ratio, ratio of IC50 for compound to IC50 for compound 1 in mouse C3H10T1/2 cells by SMO/SHH transient transcriptional activation based Gli-luciferase reporter assay	Mus musculus	10	ALA2040751	cell-based format	Scientific Literature
10.	ALA2155377	B	Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced cell differentiation after 48 hrs by alkaline phosphatase reporter assay	Mus musculus	28	ALA2150854	cell-based format	Scientific Literature
11.	ALA2155378	B	Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced cell differentiation after 48 hrs by alkaline phosphatase reporter assay relative to cyclopamine	Mus musculus	23	ALA2150854	cell-based format	Scientific Literature
12.	ALA2155379	B	Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis	Mus musculus	2	ALA2150854	cell-based format	Scientific Literature
13.	ALA2155380	B	Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis relative to cyclopamine	Mus musculus	2	ALA2150854	cell-based format	Scientific Literature
14.	ALA2155381	B	Inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of human Shh-induced Gli1 transcriptional activity after 48 hrs by RT-PCR analysis in presence of SAG	Mus musculus	3	ALA2150854	cell-based format	Scientific Literature
15.	ALA2155382	B	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli1 transcriptional activity	Mus musculus	3	ALA2150854	cell-based format	Scientific Literature
16.	ALA2155383	B	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay	Mus musculus	3	ALA2150854	cell-based format	Scientific Literature
17.	ALA2155384	B	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay in presence of SAG	Mus musculus	3	ALA2150854	cell-based format	Scientific Literature
18.	ALA2153865	B	Competitive inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 55.5 to 4500 nM by qPCR analysis	Mus musculus	1	ALA2150854	cell-based format	Scientific Literature
19.	ALA2153866	B	Competitive inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 4.1 to 1000 nM by qPCR analysis	Mus musculus	1	ALA2150854	cell-based format	Scientific Literature
20.	ALA2153868	B	Allosteric inhibition of Smo in mouse C3H10T1/2 cells assessed as inhibition of SAG-induced Gli1 transcriptional activity at 103 to 25000 nM by qPCr analysis	Mus musculus	2	ALA2150854	cell-based format	Scientific Literature