# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA971166 | B | Inhibition of platelet derived growth factor-induced endogenous association of Rac1 with GST-PAK-RBD in human U87MG cells after 5 mins by Western blot analysis | Homo sapiens | 1 | ALA1156526 | cell-based format | Scientific Literature | |
2. | ALA972151 | B | Inhibition of Rac1-PAK-RBD complex formation in platelet derived growth factor-induced human U87MG cells at 50 uM by fluorescence anisotropy | Homo sapiens | 1 | ALA1156526 | cell-based format | Scientific Literature | |
3. | ALA970413 | B | Binding affinity to Rac1 at 1 uM by fluorescence anisotropy | 1 | ALA1156526 | single protein format | Scientific Literature | ||
4. | ALA970414 | B | Inhibition of EDTA-induced nucleotide exchange on Rac1-mant-GDP by FRET assay | 1 | ALA1156526 | single protein format | Scientific Literature | ||
5. | ALA970415 | B | Inhibition of Tiam1-induced nucleotide exchange on Rac1-mant-GDP by FRET assay | 1 | ALA1156526 | single protein format | Scientific Literature | ||
6. | ALA970418 | B | Effect on Rac1 binding to RhoGDI1 expressed in 293T cells by Western blot analysis | 1 | ALA1156526 | cell-based format | Scientific Literature | ||
7. | ALA970419 | B | Effect on Rac1 binding to RhoGDI2 expressed in 293T cells by Western blot analysis | 1 | ALA1156526 | cell-based format | Scientific Literature | ||
8. | ALA970422 | B | Binding affinity at Rac1 at 1 uM by fluorescence anisotropy | 1 | ALA1156526 | single protein format | Scientific Literature | ||
9. | ALA970425 | B | Binding affinity at Rac1b at 1 uM by fluorescence anisotropy | 1 | ALA1156526 | single protein format | Scientific Literature | ||
10. | ALA1035280 | B | Inhibition of PDGF-BB-stimulated Rac1 activity in human aortic smooth muscle cells assessed as reduction of Rac1GTP level at 50 uM after 4 hrs by G-LISA relative to control | Homo sapiens | 51 | ALA1158458 | assay format | Scientific Literature | |
11. | ALA1035281 | B | Inhibition of PDGF-BB-stimulated Rac1 activity in human aortic smooth muscle cells assessed as reduction of Rac1GTP level after 4 hrs by G-LISA | Homo sapiens | 5 | ALA1158458 | assay format | Scientific Literature | |
12. | ALA1035282 | B | Inhibition of PDGF-BB-stimulated Rac1 activity in human aortic smooth muscle cells assessed as reduction of ratio of Rac1GTP/Rac1 levels at 25 uM after 4 hrs by pull-down assay | Homo sapiens | 5 | ALA1158458 | assay format | Scientific Literature | |
13. | ALA1035286 | B | Inhibition of PDGF-BB-stimulated Rac1 activity assessed as blockade of Rac1-Tiam1 interaction in human aortic smooth muscle cells at 50 uM by SDS-PAGE based chemiluminescence | Homo sapiens | 2 | ALA1158458 | assay format | Scientific Literature | |
14. | ALA1035287 | B | Inhibition of Rac1 binding to Pak1 in human aortic smooth muscle cells at 50 uM by SDS-PAGE based chemiluminescence | Homo sapiens | 3 | ALA1158458 | assay format | Scientific Literature | |
15. | ALA1035288 | B | Inhibition of Rac1 binding to Pak1 in human aortic smooth muscle cells at 100 uM by SDS-PAGE based chemiluminescence | Homo sapiens | 3 | ALA1158458 | assay format | Scientific Literature | |
16. | ALA1051382 | B | Inhibition of Rac1 GTPase activity assessed as incorporation of BODIPY-GTP at 50 uM after 40 mins by nucleotide binding competition assay | 23 | ALA1155205 | single protein format | Scientific Literature | ||
17. | ALA1053690 | B | Inhibition of Rac1 GTPase activity assessed as incorporation of BODIPY-GTP after 40 mins by nucleotide binding competition assay | 11 | ALA1155205 | single protein format | Scientific Literature | ||
18. | ALA1053693 | B | Inhibition of Rac1b GTPase activity assessed as incorporation of BODIPY-GTP at 50 uM after 40 mins by nucleotide binding competition assay | 23 | ALA1155205 | single protein format | Scientific Literature | ||
19. | ALA1053694 | B | Inhibition of Rac1b GTPase activity assessed as incorporation of BODIPY-GTP after 40 mins by nucleotide binding competition assay | 11 | ALA1155205 | single protein format | Scientific Literature | ||
20. | ALA1806007 | B | Inhibition of p38sigma MAPK assessed as residual enzyme activity at 10 uM | 1 | ALA1800061 | single protein format | Scientific Literature |