| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2061057 | B | Inhibition of human recombinant APE1 after 15 mins by fluorescence based HTS assay | Homo sapiens | 32 | single protein format | Scientific Literature | ||
| 2. | ALA2061058 | B | Inhibition of human recombinant APE1 at 57 uM after 15 mins by fluorescence based HTS assay | Homo sapiens | 23 | single protein format | Scientific Literature | ||
| 3. | ALA2061059 | B | Inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by PAGE analysis | Homo sapiens | 10 | single protein format | Scientific Literature | ||
| 4. | ALA2061060 | B | Inhibition of APE1 in human HeLa whole cell extract using [35P]-5'-AP-DNA as substrate assessed as activity remaining at 100 uM incubated for 15 mins prior to substrate addition measured after 5 mins by PAGE analysis relative to control | Homo sapiens | 10 | single protein format | Scientific Literature | ||
| 5. | ALA2061061 | B | Inhibition of APE1 in human HeLa whole cell extract using [35P]-5'-AP-DNA as substrate at 10 uM incubated for 15 mins prior to substrate addition measured after 5 mins by PAGE analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA2061062 | B | Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by Lineweaver-Burk plot analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 7. | ALA2061113 | B | Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation incubated for 10 mins prior to substrate addition measured after 5 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 8. | ALA2061114 | B | Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation at 30 uM incubated for 10 mins prior to substrate addition measured after 5 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 9. | ALA2061115 | B | Competitive inhibition of recombinant APE1 using [35P]-5'-AP-DNA as substrate assessed as decrease in APE1-DNA complex formation at 10 to 100 uM preincubated with substrate for 15 mins prior to enzyme addition measured after 5 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 10. | ALA2061116 | B | Inhibition of APE1 in human HeLa cells assessed as accumulation of genomic AP sites at 275 uM after 24 hrs by aldehyde reactive probe-based assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA2061117 | B | Inhibition of APE1 in human HeLa cells assessed as accumulation of genomic AP sites at 7.5 uM after 24 hrs by aldehyde reactive probe-based assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA2061118 | B | Inhibition of APE1 activity in human HeLa cells assessed as fold increase in genomic AP site accumulation after 24 hrs by aldehyde reactive probe-based assay in presence of methyl methanesulfonate | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 13. | ALA2061145 | B | Inhibition of human APE1 expressed in Escherichia coli rosetta using fluorescein-dabcyl-containing oligonucleotide as substrate after 5 mins by fluorescence spectrophotometry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA1021377 | B | Inhibition of purified human recombinant APE1 expressed in Escherichia coli M15 cells | Homo sapiens | 171 | cell-based format | Scientific Literature | ||
| 15. | ALA1043577 | B | Inhibition of Ape1/ref-1 redox activity in presence of 0.02 mM DTT and human Hey-C2 cells nuclear extracts by EMSA | 20 | cell-based format | Scientific Literature | |||
| 16. | ALA1043579 | B | Inhibition of Ape1/ref-1 endonuclease activity up to 100 uM | 20 | single protein format | Scientific Literature | |||
| 17. | ALA1109823 | B | Inhibition of human APE1 up to 100 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA1614283 | F | PUBCHEM_BIOASSAY: qHTS Validation Assay for Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1). (Class of assay: confirmatory) [Related pubchem assays: 1707, 1708 ] | Homo sapiens | 145 | assay format | PubChem BioAssays | ||
| 19. | ALA1614466 | F | PUBCHEM_BIOASSAY: Confirmation qHTS Assay for Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1). (Class of assay: confirmatory) [Related pubchem assays: 1705 (qHTS Validation Assay for Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1) ), 2324 (Probe Development Summary of Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1)), 1707 (Counterscreen for APE1 Inhibitors: Fluorescent Dye Displacement Validation Assay), 2517 (qHTS Assay for Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1)), 1708 (Counterscreen for APE1 Inhibitors: qHTS Validation Assay for Inhibitors of Endonuclease IV)] | Homo sapiens | 573 | assay format | PubChem BioAssays | ||
| 20. | ALA1614211 | F | PubChem BioAssay. qHTS Assay for Inhibitors of the Human Apurinic/apyrimidinic Endonuclease 1 (APE1). (Class of assay: confirmatory) | 36,499 | assay format | PubChem BioAssays |
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