# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA911945 | B | Inhibition of Mycobacterium tuberculosis MbtA by [32P]PPi-ATP exchange assay | Mycobacterium tuberculosis | 9 | ALA1138866 | single protein format | Scientific Literature | |
2. | ALA901868 | B | Inhibition of Mycobacterium tuberculosis recombinant MbtA expressed in Escherichia coli by ATP/PPi exchange assay | Mycobacterium tuberculosis | 29 | ALA1138283 | assay format | Scientific Literature | |
3. | ALA942239 | B | Inhibition of Mycobacterium tuberculosis BAG RV143 MbtA expressed in Escherichia coli BL21 (DE3) assessed as ATP-[32P]PPi exchange by liquid scintillation counting | Mycobacterium tuberculosis | 11 | ALA1152297 | assay format | Scientific Literature | |
4. | ALA999364 | B | Inhibition of Mycobacterium tuberculosis MbtA expressed in Escherichia coli assessed as ATP-[32P]Ppi exchange | Mycobacterium tuberculosis | 32 | ALA1140234 | assay format | Scientific Literature | |
5. | ALA1003925 | B | Inhibition of Mycobacterium tuberculosis recombinant MbtA expressed in Escherichia coli by ATP-PPi exchange assay | Mycobacterium tuberculosis | 24 | ALA1157357 | assay format | Scientific Literature | |
6. | ALA1003926 | B | Binding affinity to Mycobacterium tuberculosis recombinant MbtA using saturated levels of salicylic acid substrate by isothermal calorimetry | Mycobacterium tuberculosis | 1 | ALA1157357 | single protein format | Scientific Literature | |
7. | ALA1228588 | B | Inhibition of adenylation activity of Mycobacterium tuberculosis MbtA after 30 mins by ATP-[32P]pyrophosphate exchange assay | Mycobacterium tuberculosis | 1 | ALA1221167 | single protein format | Scientific Literature | |
8. | ALA4149345 | B | Inhibition of Mycobacterium tuberculosis recombinant salicylate ligase MbtA expressed in Escherichia coli using salicylic acid as substrate after 20 mins by [32P]PPi-ATP exchange based scintillation counting analysis | Mycobacterium tuberculosis | 6 | ALA4145560 | assay format | Scientific Literature | |
9. | ALA4149353 | B | Inhibition of salicylate ligase MbtA in Mycobacterium tuberculosis H37Rv in iron-free GAST medium assessed as reduction in cell-wall associated lipophilic mycobactin-T production at pH 7.4 at 5 times MIC after 27 hrs by LC-MS/MS method relative to control | Mycobacterium tuberculosis H37Rv | 1 | ALA4145560 | single protein format | Scientific Literature | |
10. | ALA4149354 | B | Inhibition of salicylate ligase MbtA in Mycobacterium tuberculosis H37Rv in iron-free GAST medium assessed as reduction in cell-wall associated lipophilic mycobactin-T production at pH 7.4 at 5 times MIC after 70 hrs by LC-MS/MS method relative to control | Mycobacterium tuberculosis H37Rv | 1 | ALA4145560 | single protein format | Scientific Literature | |
11. | ALA4149355 | B | Inhibition of salicylate ligase MbtA in Mycobacterium tuberculosis H37Rv in iron-free GAST medium assessed as reduction in water-soluble carboxymycobactin production at pH 7.4 at 5 times MIC after 27 to 70 hrs by LC-MS/MS method relative to control | Mycobacterium tuberculosis H37Rv | 1 | ALA4145560 | single protein format | Scientific Literature | |
12. | ALA5047857 | B | Binding affinity to Mycobacterium tuberculosis MbtA assessed as change in thermal stability at 100 uM incubated for 1 hr in presence of ATP at pH 7.5 by thermo fluorometer analysis | Mycobacterium tuberculosis | 2 | ALA5046254 | single protein format | Scientific Literature | |
13. | ALA5047858 | B | Binding affinity to Mycobacterium tuberculosis MbtA assessed as change in thermal stability at 100 uM incubated for 1 hr in presence of ATP at pH 8.5 by thermo fluorometer analysis | Mycobacterium tuberculosis | 2 | ALA5046254 | single protein format | Scientific Literature |