#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA955649	F	Activity at LPA5 receptor expressed in rat RH7777 cells assessed as inhibition of LPA-induced intracellular calcium concentration		12	ALA1150359	cell-based format	Scientific Literature
2.	ALA1064666	F	Antagonist activity at LPA5 receptor expressed in rat RH7777 cells assessed as inhibition of LPA-induced intracellular calcium response		8	ALA1152645	cell-based format	Scientific Literature
3.	ALA1064667	F	Antagonist activity at LPA3 receptor expressed in rat RH7777 cells assessed as inhibition of LPA-induced calcium response at 10 uM		13	ALA1152645	cell-based format	Scientific Literature
4.	ALA1064671	B	Binding affinity to LPA5		1	ALA1152645	single protein format	Scientific Literature
5.	ALA1065841	F	Agonist activity at LPA5 expressed in human chem1 cells assessed as intracellular calcium mobilization by FLIPR assay		3	ALA1159121	cell-based format	Scientific Literature
6.	ALA1065844	F	Antagonist activity at LPA5 expressed in human chem1 cells assessed as effect on intracellular calcium mobilization by FLIPR assay		1	ALA1159121	cell-based format	Scientific Literature
7.	ALA1639098	F	Agonist activity at LPA5 receptor expressed in rat B103 cells assessed as calcium mobilization at >1 uM		2	ALA1629433	cell-based format	Scientific Literature
8.	ALA2060998	F	Antagonist activity at LPA5 receptor in human washed platelets assessed as inhibition of hexadecyl-LPA-induced platelet aggregation after 3 mins	Homo sapiens	1	ALA2057121	assay format	Scientific Literature
9.	ALA2060999	F	Antagonist activity at LPA5 receptor in human isolated platelets assessed as inhibition of hexadecyl-LPA-induced platelet aggregation after 3 mins	Homo sapiens	3	ALA2057121	assay format	Scientific Literature
10.	ALA2061000	F	Antagonist activity at human recombinant LPA5 receptor expressed in rat RH7777 cells assessed as inhibition of hexadecyl-LPA-induced effect	Homo sapiens	1	ALA2057121	cell-based format	Scientific Literature
11.	ALA2344988	B	Intrinsic activity at human LPA5 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425 relative to LPA	Homo sapiens	11	ALA2331094	cell-based format	Scientific Literature
12.	ALA2344994	B	Agonist activity at human LPA5 receptor transfected in HEK293 cells after 1 hr by TGFalpha shedding assay in presence of Ki16425	Homo sapiens	11	ALA2331094	cell-based format	Scientific Literature
13.	ALA3363677	F	Agonist activity at FLAG-tagged human LPA5 expressed in rat B103 cells by Fura-2AM dye based Ca2+ mobilization assay	Homo sapiens	16	ALA3352405	cell-based format	Scientific Literature
14.	ALA3363954	F	Agonist activity at FLAG-tagged human LPA5 expressed in rat B103 cells by Fura-2AM dye based Ca2+ mobilization assay relative to LPA 18:1	Homo sapiens	15	ALA3352405	cell-based format	Scientific Literature
15.	ALA3624410	F	Antagonist activity at human LPA5 receptor expressed in rat B103 cells assessed as inhibition of LPA-induced calcium mobilization preincubated for 30 mins followed by LPA induction by FLIPR Calcium 4 dye-based fluorometric analysis	Homo sapiens	1	ALA3621155	cell-based format	Scientific Literature
16.	ALA3889171	F	Receptor Assay: Engagement of the LPA5 receptor by its ligand, oleoyl-L-α-lysophosphatidic acid (LPA), leads to the release of intracellular stores of calcium into the cytoplasm mediated through a Gq-driven increase in inositol triphosphate (IP3) levels. Gq is a heterotrimeric G protein subunit that activates phospholipase C.The ability of test compounds to prevent LPA-driven intracellular release of stored calcium from RH7777 cells expressing human LPA5 receptors was assayed by stimulating the cells with LPA in the presence of various test compounds to measure the test compounds' antagonism, i.e. activity in vitro. Approximately 10,000 cells in normal culture medium (Minimal Essential Media, 10% Foetal Calf Serum) were dispensed per assay well in a black clear bottom poly-D-lysine coated 384 well plate (Corning). Cells were allowed to settle for thirty minutes at room temperature before being incubated overnight at 37° C., 5% CO2. Sixteen to twenty hours after dispensing, the cells were loaded with a calcium-sensitive fluorescent dye by replacing the culture medium with assay buffer (1x Hanks buffered saline, 25 mM HEPES, 0.1% w/v fatty acid free BSA (bovine serum albumin), pH7.4) containing 1.25 mM probenecid and 1x Calcium 5 Reagent (Molecular Devices). Cells were incubated at 37° C. for one hour to allow for dye uptake.		83	ALA3886973	cell-based format	BindingDB Database
17.	ALA3998928	F	Agonist activity at FLAG-tagged LPA5 receptor (unknown origin) expressed in rat B103 cells assessed as increase in intracellular calcium level up to 10 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay	Homo sapiens	3	ALA3997796	cell-based format	Scientific Literature
18.	ALA3998934	F	Antagonist activity at FLAG-tagged LPA5 receptor (unknown origin) expressed in rat B103 cells assessed as inhibition of LPA-induced intracellular calcium level up to 100 uM measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay	Homo sapiens	5	ALA3997796	cell-based format	Scientific Literature
19.	ALA3998951	F	Agonist activity at FLAG-tagged LPA5 receptor (unknown origin) expressed in rat B103 cells assessed as increase in intracellular calcium level measured every 3.42 secs for 70 secs by Fura-2-AM dye based fluorescence assay	Homo sapiens	1	ALA3997796	cell-based format	Scientific Literature
20.	ALA4432721	F	Agonist activity at LPA receptor 5 (unknown origin) assessed as calcium mobilization after 30 mins by fluorescence analysis	Homo sapiens	1	ALA4431364	single protein format	Scientific Literature