#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1920920	B	Binding affinity for GST-tagged mouse MCL1 expressed in Escherichia coli BL21 cells	Mus musculus	2		cell-based format	Scientific Literature
2.	ALA1921249	B	Displacement of BODIPY-Bak conjugated peptide from GST-tagged mouse wild type MCL1 expressed in Escherichia coli BL21 cells at 10 uM after 3 hrs by fluorescence polarization competition assay	Mus musculus	6		cell-based format	Scientific Literature
3.	ALA989995	B	Binding affinity to mouse Mcl-1 expressed in Escherichia coli BL21 cells	Mus musculus	9		cell-based format	Scientific Literature
4.	ALA1120825	B	Displacement of FITC-labeled BH3 peptide from GST-tagged mouse Mcl1 after 1 hr by fluorescence polarization assay	Mus musculus	5		single protein format	Scientific Literature
5.	ALA3267198	B	Inhibition of MCL1 in wild-type MEF assessed as induction of BAX/BAK-dependent cell death after 15 hrs by propidium iodide staining-based flow cytometric analysis	Mus musculus	1		single protein format	Scientific Literature
6.	ALA3267199	B	Inhibition of MCL1 in BAX/BAK double knock out MEF assessed as induction of cell death at 16 uM after 15 hrs by propidium iodide staining-based flow cytometric analysis relative to control	Mus musculus	1		single protein format	Scientific Literature
7.	ALA3267200	B	Inhibition of MCL1 in wild-type MEF assessed as induction of BAX/BAK-dependent cell death at 16 uM after 15 hrs by propidium iodide staining-based flow cytometric analysis relative to control	Mus musculus	1		single protein format	Scientific Literature
8.	ALA3822343	B	Inhibition of biotinylated Bim BH3 peptide binding to recombinant GST-tagged mouse MCL1 with N-terminal truncation of 151 residues and C-terminal truncation of 23 residues expressed in Escherichia coli BL21(DE3) cells preincubated for 1 hr followed by biotinylated Bim BH3 peptide addition measured after 1 hr by ELISA	Mus musculus	1		cell-based format	Scientific Literature
9.	ALA3888759	B	Fluorescence Polarization-Based Binding Assay: Sensitive and quantitative FP-based binding assays were developed and optimized to determine the binding affinities of small-molecule inhibitors to the recombinant Mcl-1, A1/Bfl-1, Bcl-w, Bcl-2, and Bcl-xL proteins. The concentrations of the proteins used in the competitive binding experiments were 10 nM for Mcl-1, 80 nM for Bcl-xL, and 60 nM for Bcl-2. The fluorescent probes, Flu-BID or FAM-BID were fixed at 2 nM for all assays, which binds with Kd values of 2.3 nM, 14.2 nM and 24.1 nM against Mcl-1, Bcl-2 and Bcl-xL respectively. 5 μL of the tested compound in DMSO and 120 μL of protein/probe complex in the assay buffer (20 mM phosphate pH 7.4, 50 mM NaCl, 1 mM EDTA. 0.05% Pluronic F68)s) were added to 96 well black assay plates, incubated at room temperature for 3 h and the polarization values (mP) were measured at an excitation wavelength at 485 nm and an emission wavelength at 530 nm using the plate reader Synergy H1 Hybrid, BioTek.	Mus musculus	23		single protein format	BindingDB Database
10.	ALA4052835	B	Inhibition of biotinylated Bim BH3 peptide binding to GST-tagged mouse Mcl-1 expressed in Escherichia coli BL21 (DE3) pre-incubated for 1 hr followed by incubation with shaking for 2 hrs and subsequent Bim BH3 peptide addition measured after 1.5 hrs by ELISA	Mus musculus	1		assay format	Scientific Literature
11.	ALA4147746	B	Inhibition of biotin labelled Bid-BH3 binding to N-terminal truncated 151/C-terminal truncated of 23 residues GST-tagged mouse MCL1 expressed in Escherichia coli BL2(DE3) preincubated for 1 hr followed by followed by biotin labelled Bid-BH3 addition measured after 2 hrs by ELISA	Mus musculus	1		assay format	Scientific Literature
12.	ALA4148755	B	Inhibition of FITC-Ahx-Bid-OH binding to mouse DN150/DC25 Mcl-1 after 2 hrs by fluorescent polarization assay	Mus musculus	33		single protein format	Scientific Literature
13.	ALA5035033	B	Inhibition of biotinylated Bim BH3 peptide binding to GST-tagged mouse MCL1 expressed in Escherichia coli BL21 (DE3) incubated for 1 hr by ELISA	Mus musculus	1		assay format	Scientific Literature