#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2026780	B	Inhibition of mouse recombinant FAP expressed in HEK293 cells assessed as pNA release from Ala-Pro-p-nitroanilide pre-incubated with enzyme for 15 mins prior to substrate addition by fluorescence technique	Mus musculus	40		cell-based format	Scientific Literature
2.	ALA997753	B	Activity of mouse FAP expressed in HEK293 cells assessed as enzyme-mediated drug cleavage after 24 hrs by confocal microscopy relative to control	Mus musculus	1		cell-based format	Scientific Literature
3.	ALA997755	B	Activity of mouse FAP expressed in HEK293 cells assessed as enzyme-mediated drug activation by measuring intracellular fluorescence after 24 hrs by confocal microscopy relative to control	Mus musculus	1		cell-based format	Scientific Literature
4.	ALA997756	B	Activity of mouse FAP expressed in HEK293 cells assessed as enzyme-mediated drug activation by measuring intracellular fluorescence after 1 hr by confocal microscopy relative to control	Mus musculus	1		cell-based format	Scientific Literature
5.	ALA997757	B	Activity of mouse FAP expressed in HEK293 cells assessed as enzyme-mediated drug activation by measuring intracellular fluorescence after 6 hrs by confocal microscopy	Mus musculus	2		cell-based format	Scientific Literature
6.	ALA997759	B	Ratio of Kcat to Km for mouse FAP assessed as enzyme mediated drug cleavage by measuring increase in fluorescence intensity	Mus musculus	1		single protein format	Scientific Literature
7.	ALA997764	B	Binding affinity to mouse FAP assessed as enzyme mediated drug cleavage by measuring increase in Pyro fluorescence	Mus musculus	1		single protein format	Scientific Literature
8.	ALA1002880	B	Activity of mouse FAP assessed as enzyme mediated drug cleavage by measuring increase in fluorescence intensity	Mus musculus	1		single protein format	Scientific Literature
9.	ALA1003612	B	Binding affinity to mouse FAP assessed as enzyme mediated drug cleavage by measuring Pyro fluorescence	Mus musculus	2		single protein format	Scientific Literature
10.	ALA2185388	B	Inhibition of mouse recombinant FAP expressed in HEK293 cells assessed as pNA release from Ala-Pro-p-nitroanilide pre-incubated with enzyme for 15 mins prior to substrate addition by fluorescence technique	Mus musculus	32		cell-based format	Scientific Literature
11.	ALA2345663	B	Inhibition of mouse FAP expressed in human HEK293 cells using N-terminal blocked Z-GP-AMC substrate at 0.036 uM to 1 uM preincubated for 10 mins prior to substrate addition by fluorescence assay in presence of prolyl endopeptidase	Mus musculus	1		cell-based format	Scientific Literature
12.	ALA2389863	B	Inhibition of mouse recombinant FAP expressed in HEK293 cells using Ala-Pro-p-nitroanilide as substrate incubated for 15 mins prior to substrate addition	Mus musculus	35		cell-based format	Scientific Literature
13.	ALA3239280	B	Inhibition of recombinant mouse FAP purified from HEK293 cell supernatant using Ala-Pro-p-nitroanilide as substrate by spectrophotometry	Mus musculus	60		single protein format	Scientific Literature
14.	ALA3239286	B	Irreversible inhibition of recombinant mouse FAP purified from HEK293 cell supernatant using Ala-Pro-p-nitroanilide as substrate by kinetic study	Mus musculus	1		single protein format	Scientific Literature
15.	ALA3239069	B	Reversible inhibition of recombinant mouse FAP purified from HEK293 cell supernatant using Ala-Pro-p-nitroanilide as substrate by kinetic study	Mus musculus	3		single protein format	Scientific Literature
16.	ALA3802986	B	Inhibition of BALB/c mouse 5'-His6-tagged 3'-FLAG-tagged FAP fused with ECD expressed in HEK293 cells using Ala-Pro-p-nitroanilide as substrate preincubated for 15 mins followed by substrate addition measured for 10 mins by spectrofluorimetry	Mus musculus	5		cell-based format	Scientific Literature
17.	ALA3888519	B	Enzymatic Assay: Enzyme activities were determined kinetically in a final volume of 200 μl for 10 minutes at 37° C. by measuring the initial velocities of pNA release (405 nm) from the substrate using a Spectramax plus microtiterplate reader (Molecular devices). One unit of enzyme activity was defined as the amount of enzyme that catalyzes the release of 1 μmol pNA from the substrate per minute under assay conditions. The chromogenic substrate Gly-Pro-p-nitroanilide (100 μmol/l) was used at pH 8.3 for DPP IV, Lys-Ala-p-nitroanilide (1 mmol/l) at pH 5.5 for DPP II, Ala-Pro-p-nitroanilide (300 μmol/l) at pH 7.4 for DPP9 and Ala-Pro-p-nitroanilide (2 mmol/l) at pH 7.4 for FAP activity measurement. To evaluate the endopeptidase activity of FAP and the influence of inhibitors thereon, Z-Gly-Pro-AMC and Z-Gly-Pro-p-nitroanilide were used at a final concentration of 300 and 100 μmol/l, respectively. The substrate concentrations were chosen around the Km value obtained under the assay conditions used. Buffer compositions for the DPP assays were reported before in the purification articles−vide supra. The FAP assay buffer consisted of 50 mM Tris pH7.4 containing 100 mmol/l NaCl and 0.1 mg/ml bovine serum albumin. The PREP activity was measured as described by Brandt et al. using the chromogenic substrate Z-Gly-Pro-p-nitroanilide (0.25 mmol/l) at pH 7.5 in the presence of 10 mmol/l DTT. Test compounds were dissolved and diluted in DMSO (final concentration DMSO during assay 5% v/v) except for FAP where dilution of the inhibitor was done in water. Inhibitors are pre-incubated with the enzyme for 15 min at 37° C. before starting the assay by the addition of substrate. The concentration of enzyme and of inhibitor during the preincubation is double of the final concentration during activity measurement.		49		single protein format	BindingDB Database