# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3630630 | Binding | Inhibition of AKT2 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control | Homo sapiens | 1 | ALA3627674 | single protein format | Scientific Literature | |
2. | ALA3637595 | Binding | Induction of Akt phosphorylation in mouse C2C12 cells at 30 ug/ml pretreated with compound for 24 hrs followed by fresh medium with compound addition measured after 2 hrs by Western blot analysis | Mus musculus | 4 | ALA3632548 | cell-based format | Scientific Literature | |
3. | ALA3734279 | Binding | Inhibition of Akt1 in mouse BetaTC6 cells assessed as increase in insulin release at 10 uM incubated for 2 hrs by ELISA | Mus musculus | 1 | ALA3727361 | cell-based format | Patent Bioactivity Data | |
4. | ALA3737513 | Binding | Inhibition of AKT2 (unknown origin) at 1 uM by selectscreen kinase profiling assay in presence of ATP | Homo sapiens | 1 | ALA3734692 | single protein format | Scientific Literature | |
5. | ALA3743158 | Binding | Inhibition of AKT phosphorylation in Nf1 defective and Trp53 defective mouse K1492 cells | Mus musculus | 2 | ALA3739310 | cell-based format | Scientific Literature | |
6. | ALA3743159 | Binding | Inhibition of AKT phosphorylation in Nf1 defective and Trp53 defective mouse K5001 cells | Mus musculus | 2 | ALA3739310 | cell-based format | Scientific Literature | |
7. | ALA3772347 | Binding | Effect on Akt phosphorylation in mouse RAW264.7 cells transfected with empty vector at 10 to 40 nM pre-treated for 1 hrs followed by stimulation with IFNgamma for 1 hr by Western blotting method | Mus musculus | 1 | ALA3769383 | cell-based format | Scientific Literature | |
8. | ALA3803715 | Binding | Inhibition of LPS-induced AKT phosphorylation in mouse RAW264.7 cells at 1 to 50 uM pre-incubated for 30 mins before LPS stimulation for 30 hrs by Western blotting method | Mus musculus | 1 | ALA3797147 | cell-based format | Scientific Literature | |
9. | ALA3821463 | Binding | Inhibition of Akt phosphorylation in mouse NIH/3T3-K-RAS cells at 100 uM pretreated for 1 hr by Western blot analysis | Mus musculus | 3 | ALA3817785 | cell-based format | Scientific Literature | |
10. | ALA3821464 | Binding | Inhibition of serum-stimulated Akt phosphorylation in mouse NIH/3T3-K-RAS cells at 100 uM pretreated for 1 hr followed by serum addition for 30 mins by Western blot analysis | Mus musculus | 3 | ALA3817785 | cell-based format | Scientific Literature | |
11. | ALA1116495 | Binding | Induction of Akt activity in 24 hrs serum starved mouse Mel-Ab cells at 50 uM by Western blot analysis | Mus musculus | 1 | ALA1158586 | cell-based format | Scientific Literature | |
12. | ALA1244164 | Binding | Inhibition of CD-28 triggered Akt phosphorylation in mouse DWT6.11 cells assessed as enzyme phosphorylation at Thr308 by immunoblotting relative to control | Mus musculus | 1 | ALA1240339 | cell-based format | Scientific Literature | |
13. | ALA1244165 | Binding | Inhibition of CD-28 triggered Akt phosphorylation in mouse DWT6.11 cells assessed as enzyme phosphorylation at Ser473 by immunoblotting relative to control | Mus musculus | 1 | ALA1240339 | cell-based format | Scientific Literature | |
14. | ALA1687390 | Binding | Displacement of biotinylated phosphatidylinositol-3,4,5-phosphate from mouse AKT1 PH domain by surface plasmon resonance competitive binding assay | Mus musculus | 49 | ALA1681784 | single protein format | Scientific Literature | |
15. | ALA1050133 | Binding | Inhibition of Akt phosphorylation in mouse Sal cells by Western blotting | Mus musculus | 1 | ALA1154171 | cell-based format | Scientific Literature | |
16. | ALA941477 | Binding | Increase in Akt phosphorylation in TSC1-deficient mouse embryo fibroblasts assessed as Ser 473 phosphorylation at 100 nM after 24 hrs by Western blot | Mus musculus | 1 | ALA1156476 | single protein format | Scientific Literature | |
17. | ALA1028926 | Binding | Inhibition of mouse AKT1 | Mus musculus | 1 | ALA1140130 | single protein format | Scientific Literature | |
18. | ALA1653178 | Binding | Inhibition of Akt phosphorylation in mouse RAW264.7 cells infected with Salmonella enterica serovar Typhimurium ATCC 14028 assessed as decrease of p-473Ser-Akt level after 8 hrs by Western blotting | Mus musculus | 1 | ALA1649299 | cell-based format | Scientific Literature | |
19. | ALA1653179 | Binding | Inhibition of Akt phosphorylation in mouse RAW264.7 cells infected with Salmonella enterica serovar Typhimurium ATCC 14028 assessed as decrease of p-308Thr-Akt level after 8 hrs by Western blotting | Mus musculus | 1 | ALA1649299 | cell-based format | Scientific Literature | |
20. | ALA1653180 | Binding | Inhibition of Akt phosphorylation in mouse RAW264.7 cells infected with Salmonella enterica serovar Typhimurium ATCC 14028 assessed as decrease of phosphorylated GSK3-beta level at 1 uM after 8 hrs by Western blotting | Mus musculus | 1 | ALA1649299 | cell-based format | Scientific Literature |