#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1060467	Binding	Transcriptional activation of HSF1 in heat shock stress-induced human HeLa cells assessed as granule formation treated 1 hr before heat shock challenge measured after 2 hrs by immunocytochemical staining	Homo sapiens	44	ALA1152919	cell-based format	Scientific Literature
2.	ALA1060469	Binding	Transcriptional activation of HSF1 in human HeLa cells assessed as granule formation treated 3 hrs before heat shock challenge by immunocytochemical staining	Homo sapiens	46	ALA1152919	cell-based format	Scientific Literature
3.	ALA1051570	Binding	Transcriptional activation of HSF1 in heat shock stress-induced human HeLa cells assessed as granule formation at 80 uM treated 1 hr before heat shock challenge measured after 2 hrs by immunocytochemical staining	Homo sapiens	4	ALA1152919	cell-based format	Scientific Literature
4.	ALA1063218	Binding	Amplification of HSF1 transcriptional activity in human HeLa cells assessed as granule formation pretreated for 3 hrs by immunocytochemical staining	Homo sapiens	27	ALA1152353	cell-based format	Scientific Literature
5.	ALA1063219	Binding	Amplification of HSF1 transcriptional activity in human heat shock-induced HeLa cells assessed as granule formation treated 1 hr before heat shock challenge measured after 2 hrs without recovery time by immunocytochemical staining	Homo sapiens	26	ALA1152353	cell-based format	Scientific Literature
6.	ALA1063232	Binding	Amplification of HSF1 transcriptional activity in human HeLa cells assessed as HFS1 granule count at 80 uM by immunocytochemical staining	Homo sapiens	1	ALA1152353	cell-based format	Scientific Literature
7.	ALA993711	Binding	Inhibition of HSF1 binding to heat shock element DNA assessed as HSF1/HSE complex formation in human HeLa cells at 50 ug/mL treated for 1 hr before heat induction	Homo sapiens	1	ALA1153987	cell-based format	Scientific Literature
8.	ALA993712	Binding	Inhibition of HSF1 binding to heat shock element DNA assessed as HSF1/HSE complex formation in human Jurkat cells at 50 ug/mL treated for 1 hr before heat induction	Homo sapiens	1	ALA1153987	cell-based format	Scientific Literature
9.	ALA3295291	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of hsp25 promoter activity after 12 hrs by luciferase reporter gene assay	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
10.	ALA3295292	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of hsp70 promoter activity after 12 hrs by luciferase reporter gene assay	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
11.	ALA3295295	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP27 mRNA expression at 1 to 20 uM after 12 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
12.	ALA3295296	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP70 mRNA expression at 1 to 20 uM after 12 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
13.	ALA3295297	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP1 mRNA expression at 1 to 20 uM after 12 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
14.	ALA3295298	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP27 mRNA expression at 20 uM up to 24 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
15.	ALA3295299	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP70 mRNA expression at 20 uM up to 24 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
16.	ALA3295300	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP1 mRNA expression at 20 uM up to 24 hrs by qRT-PCR analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
17.	ALA3295301	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP25 protein expression after 12 hrs by Western blot analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
18.	ALA3295302	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP70 protein expression after 12 hrs by Western blot analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
19.	ALA3295555	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP1 protein expression after 12 hrs by Western blot analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature
20.	ALA3295556	Binding	Inhibition of HSP1 activity in human NCI-H460 cells assessed as reduction of HSP25 protein expression at 20 uM by Western blot analysis	Homo sapiens	1	ALA3286220	cell-based format	Scientific Literature