| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA3562016 | Functional | PubChem BioAssay. MLPCN ERAP1 Measured in Biochemical System Using Plate Reader - 7016-01_Inhibitor_Dose_DryPowder_Activity_Set2. (Class of assay: confirmatory) | ⋯ | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 2. | ALA3562042 | Functional | PubChem BioAssay. MLPCN ERAP1 Measured in Biochemical System Using Plate Reader - 7016-01_Inhibitor_Dose_DryPowder_Activity. (Class of assay: confirmatory) | 3 | ALA1201862 | biochemical format | PubChem BioAssays | ||
| 3. | ALA3829450 | Binding | Inhibition of recombinant human ERAP1 using L-AMC as substrate measured for 15 to 30 mins by fluorescence analysis | Homo sapiens | 2 | ALA3826883 | single protein format | Scientific Literature | |
| 4. | ALA3829452 | Binding | Inhibition of human ERAP1 preincubated for 30 to 60 mins followed by addition of Leu-AMC as substrate measured for 15 mins by spectrofluorimetric method | Homo sapiens | 47 | ALA3826883 | single protein format | Scientific Literature | |
| 5. | ALA4358832 | Binding | Activation of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated fluorogenic Leu-AMC substrate hydrolysis after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 6. | ALA4358833 | Binding | Activation of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated antigenic epitope destruction assessed as trimming of 9mer peptide substrate YTAFTIPSI to 8mer peptide TAFTIPSI at 100 uM after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 7. | ALA4358836 | Binding | Allosteric competitive activation of full length C-terminal His6-tagged ERAP1 (unknown origin) assessed as decrease in K prime without change in Vmax at 400 to 800 nM using varying level of Leu-pNA as substrate measured up to 60 mins by Michaelis-Menten analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 8. | ALA4358837 | Binding | Activation of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated fluorogenic Leu-AMC substrate hydrolysis after 60 mins RapidFire high-throughput mass spectrometry assay relative to control | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 9. | ALA4358840 | Binding | Inhibition of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated antigenic epitope destruction assessed as inhibition of trimming of 9mer peptide substrate YTAFTIPSI to 8mer peptide TAFTIPSI after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 10. | ALA4358841 | Binding | Competitive inhibition of full length C-terminal His6-tagged ERAP1 (unknown origin) assessed as change in substrate Km without changing Vmax at 5 to 15 uM using HIV1 Gag-polyprotein YTAFTIPSI as substrate measured up to 60 mins by Michaelis-Menten analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 11. | ALA4358842 | Binding | Inhibition of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated chicken ovalbumin antigenic epitope processing assessed as reduction in SIINFEKL generation using SGLEQLESIINFEKL as substrate measured after 30 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 12. | ALA4358843 | Binding | Inhibition of full length C-terminal His6-tagged ERAP1 (unknown origin)-mediated chicken ovalbumin antigenic epitope processing assessed as reduction in generation of mature antigenic epitopes of length ranging from 8mer to 13mer using SGLEQLESIINFEKL as substrate by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 13. | ALA4358844 | Binding | Inhibition of ERAP1-mediated antigen epitope presentation in human HeLa cells transfected with Bacmam virus containing mouse MHC-1 allele H-2 Kb, beta2m and LEQLESIINFEKL epitope assessed as reduction in H-2 Kb/SIINFEKL complex at cell surface after 40 hrs by 25D1.16 staining based flow cytometry analysis | Homo sapiens | 1 | ALA4354860 | cell-based format | Scientific Literature | |
| 14. | ALA4358846 | Binding | Activation of full length C-terminal His6-tagged ERAP1 Y684F mutant (unknown origin)-mediated substrate hydrolysis using Leu-AMC as substrate after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 15. | ALA4358847 | Binding | Activation of full length C-terminal His6-tagged ERAP1 K685A mutant (unknown origin)-mediated substrate hydrolysis using Leu-AMC as substrate after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 16. | ALA4358848 | Binding | Activation of full length C-terminal His6-tagged ERAP1 K685M mutant (unknown origin)-mediated substrate hydrolysis using Leu-AMC as substrate after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 17. | ALA4358849 | Binding | Activation of full length C-terminal His6-tagged ERAP1 Y684F/K685A double mutant (unknown origin)-mediated substrate hydrolysis using Leu-AMC as substrate after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 18. | ALA4358850 | Binding | Activation of human recombinant ERAP1 assessed as induction of substrate hydrolysis at 10 uM using Leu-AFC as substrate measured after 60 mins by fluorescence based analysis relative to control | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 19. | ALA4358851 | Binding | Activation of human recombinant ERAP1 assessed as induction of substrate hydrolysis at 20 uM using Leu-AFC as substrate measured after 60 mins by fluorescence based analysis relative to control | Homo sapiens | 1 | ALA4354860 | single protein format | Scientific Literature | |
| 20. | ALA3418218 | Binding | Inhibition of human recombinant ERAP1 expressed in baculovirus infected Sf9 cells using L-leucine-7-amido-4-methyl coumarin as substrate up to 100 uM after 5 to 10 mins by fluorescence assay | Homo sapiens | 34 | ALA3414429 | cell-based format | Scientific Literature |
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