#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2060020	B	Binding affinity to Hsp27 in human SKBR3 cell lysate at 25 uM after 30 mins by mass spectrometric analysis	Homo sapiens	1		cell-based format	Scientific Literature
2.	ALA2060023	B	Binding affinity to pure Hsp27 in human SKBR3 cell lysate at 25 to 50 uM after 1 hr by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
3.	ALA2060030	B	Induction of Hsp27 phosphorylation in human SKBR3 cells at 0.2 to 1 uM after 24 hrs by SDS-PAGE analysis	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA2060031	B	Inhibition of Hsp27 phosphorylation in human SKBR3 cells at 0.5 to 1 uM after 24 hrs by immunofluorescence analysis	Homo sapiens	1		cell-based format	Scientific Literature
5.	ALA2060033	B	Binding affinity to pure Hsp27 in human SKBR3 cell lysate at 0.5 to 1 uM after 1 hr by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
6.	ALA2060036	B	Induction of Hsp27 phosphorylation in human SKBR3 cells at 50 to 100 nM after 24 hrs by SDS-PAGE analysis	Homo sapiens	1		cell-based format	Scientific Literature
7.	ALA987300	B	Inhibition of HSP27 phosphorylation in IL-1-alpha-stimulated HLF cells	Homo sapiens	6		cell-based format	Scientific Literature
8.	ALA993710	B	Enhancement of HSP27 Ser78 phosphorylation in human HeLa cells at 50 ug/mL treated for 1 hr	Homo sapiens	11		cell-based format	Scientific Literature
9.	ALA955776	B	Inhibition of LPS-stimulated hsp27 phosphorylation in 1 hr pretreated human THP1 cells by Western blot	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA2399253	B	Inhibition of HSP27 (unknown origin) assessed as increase in DTT-induced insulin B chain aggregation at 5 uM incubated for 5 mins prior to DTT-treatment measured after 30 mins by spectrophotometric analysis relative to control	Homo sapiens	1		single protein format	Scientific Literature
11.	ALA2399958	B	Inhibition of HSP27 (unknown origin) assessed as increase in DTT-induced insulin B chain aggregation at 10 uM incubated for 5 mins prior to DTT-treatment measured after 30 mins by spectrophotometric analysis relative to control	Homo sapiens	5		single protein format	Scientific Literature
12.	ALA3587752	B	Inhibition of HSP27 (unknown origin) by insulin aggregation assay	Homo sapiens	1		single protein format	Scientific Literature
13.	ALA4026126	B	Inhibition of heat shock protein 27 in human LNCaP cells assessed as decrease in AR protein level at 200 uM after 48 hrs by Western blot method	Homo sapiens	3		cell-based format	Scientific Literature
14.	ALA4026127	B	Inhibition of heat shock protein 27 in human LNCaP cells assessed as decrease in AR protein level at 20 uM after 48 hrs by Western blot method	Homo sapiens	7		cell-based format	Scientific Literature
15.	ALA4026128	B	Inhibition of heat shock protein 27 in human LNCaP cells assessed as decrease in AR protein level after 48 hrs by Western blot method	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4026130	B	Inhibition of heat shock protein 27 in human LNCaP cells assessed as decrease in AR protein level at 5 uM after 48 hrs by Western blot method	Homo sapiens	1		cell-based format	Scientific Literature
17.	ALA4133455	B	Induction of abnormal HSP27 dimerization in human NCI-H460 cells at 10 uM co-treated with 17-AAG after 24 hrs by Western blot analysis	Homo sapiens	4		cell-based format	Scientific Literature
18.	ALA4133456	B	Induction of abnormal HSP27 dimerization in human NCI-H460 cells at 10 uM after 12 hrs by Western blot analysis	Homo sapiens	4		cell-based format	Scientific Literature
19.	ALA4133457	B	Induction of abnormal recombinant HSP27 (unknown origin) dimerization after 3 hrs by Western blot analysis	Homo sapiens	8		single protein format	Scientific Literature
20.	ALA4133460	B	Induction of abnormal HSP27 dimerization in human NCI-H460 cells assessed as potentiation of 17-AAG-induced antiproliferative activity at 10 uM after 24 hrs by EZ-CYTOX cell viability assay	Homo sapiens	2		cell-based format	Scientific Literature