Biodistribution in wild type FVB Mdr1a/Mdr1b positive mouse hypothalamus assessed as tritiated drug level at 150 uM perfusion after 30 mins by capillary depletion assay
Biodistribution in FVB Mdr1a/Mdr1b deficient mouse hypothalamus assessed as tritiated drug level at 150 uM perfusion after 30 mins by capillary depletion assay
Effect on POMC gene expression in Sabra mouse hypothalamus assessed as increase in mRNA level at 1 mg/kg, ip administered on day 6 to 10 measured on day 16 by RT-PCR analysis relative to control
Effect on POMC gene expression in ip dosed Sabra mouse hypothalamus assessed as increase in mRNA level administered on day 6 to 10 measured on day 16 by RT-PCR analysis relative to control
Effect on FAAH gene expression in Sabra mouse hypothalamus assessed as change in mRNA level at 1 mg/kg, ip administered on day 8, 9, 10, 11, 14, 18, 24 and 32 by RT-PCR analysis relative to GAPDH
Effect on CAMKK2 gene expression in Sabra mouse hypothalamus assessed as change in mRNA level at 1 mg/kg, ip administered on day 8, 9, 10, 11, 14, 18, 24 and 32 by RT-PCR analysis relative to GAPDH
Effect on 5HT1A gene expression in Sabra mouse hypothalamus assessed as change in mRNA level at 1 mg/kg, ip administered on day 8, 9, 10, 11, 14, 18, 24 and 32 by RT-PCR analysis relative to GAPDH
Effect on CART gene expression in Sabra mouse hypothalamus assessed as change in mRNA level at 1 mg/kg, ip administered on day 8, 9, 10, 11, 14, 18, 24 and 32 by RT-PCR analysis relative to GAPDH